2012 Promega catalogue

Cell Signaling
BacTiter-Glo Microbial Cell Viability Assay
Product Size Cat.# Price ($)
BacTiter-Glo Microbial Cell Viability Assay 10 ml G8230 140.00
For Laboratory Use.
10 × 10 ml G8231 1120.00
100 ml G8232 979.00
10 × 100 ml G8233 Pls. Enq.
Description: The BacTiter-Glo Microbial Cell Viability Assay is a homogeneous
method for determining the number of viable microbial cells in culture
based on quantitation of the ATP present. ATP is an indicator of metabolically
active cells. The homogeneous assay procedure involves adding a single
reagent directly to bacterial cells cultured in medium and measuring luminescence.
The formulation of the reagent supports bacterial cell lysis and generation
of a luminescent signal in a homogeneous “add-mix-measure” format.
The luminescent signal is proportional to the amount of ATP present, which is
directly proportional to the number of viable cells in culture. The assay relies on
a proprietary thermostable luciferase (Ultra-Glo Recombinant Luciferase) and
a proprietary buffer formulation for extracting ATP from bacteria. The assay has
been shown to detect a variety of bacteria and fungi.
Features:
• Simplify Microbial Detection: The “add-mix-measure” format reduces
the number of handling steps with no separate lysis step, and no injectors
required, allowing easy automation.
• Get Results Quickly: Data can be recorded in 5 minutes or less after
adding reagent and mixing. Superior sensitivity allows you to detect growth
or toxicity quickly after inoculation.
• Increase Your Sensitivity: Measure ATP from as few as 10 bacterial
cells, 1,000-fold more sensitive than absorbance (O.D.) readings.
• Choose Your Format: Can be used with various multiwell-plate or singleuse
formats. Data can be recorded by luminometer or CCD camera.
• Process Plates Consecutively: The “glow-type” luminescent signal is
stable, with a half-life generally over 30 minutes.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: For long-term storage, the lyophilized BacTiter-Glo
Substrate and BacTiter-Glo Buffer should be stored at –20°C.
Protocol Part#
BacTiter-Glo Microbial Cell Viability Assay Technical Bulletin TB337
For complete and up-to-date product information visit: www.promega.com/catalog
BacTiter-Glo
Reagent
Mixer
Luminometer
Diagram of the BacTiter-Glo Microbial Cell Viability Assay protocol.
Luminescence (RLU)
102 103 104 105 106 107 108 109 BacTiter-Glo Assay
O.D. 600
10.00
1.00
0.10
Time (hours)
10
0 5 10 15 20 25
Time (hours)
4609MB
0.01
0 5 10 15 20 25
Evaluate bacterial growth immediately after inoculation using the
BacTiter-Glo Assay. When measuring growth by O.D., the first significant
measurement (0.25 O.D. with E. coli) did not occur until 5 hours after
inoculation.
4616MC
35
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Cell Health Assays
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