2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Cell Viability Assays
34
CellTiter-Glo ® Luminescent Cell Viability
Assay
Product Size Cat.# Price ($)
CellTiter-Glo ® Luminescent Cell Viability
Assay
For Laboratory Use.
10 ml G7570 137.00
10 × 10 ml G7571 1098.00
100 ml G7572 960.00
10 × 100 ml G7573 Pls. Enq.
Description: The CellTiter-Glo ® Luminescent Cell Viability Assay is a homogeneous
method of determining the number of viable cells in culture based on
quantitation of the ATP present, an indicator of metabolically active cells. The
CellTiter-Glo ® Assay is designed for use with multiwell formats, making it ideal
for automated high-throughput screening (HTS), cell proliferation and cytotoxicity
assays. The homogeneous assay procedure involves adding the single reagent
(CellTiter-Glo ® Reagent) directly to cells cultured in serum-supplemented
medium. Cell washing, removal of medium and multiple pipetting steps are not
required. The system detects as few as 15 cells/well in a 384-well format in 10
minutes after adding reagent and mixing.
Adding the single reagent results in cell lysis and generation of a luminescent
signal proportional to the amount of ATP present. The amount of ATP is directly
proportional to the number of cells present in culture. The CellTiter-Glo ® Assay
generates a “glow-type” luminescent signal, which has a half-life generally
greater than five hours, depending on cell type and medium used. The
extended half-life eliminates the need to use injectors and provides flexibility for
continuous or batch mode processing of multiple plates. The unique homogeneous
format avoids errors that may be introduced by other ATP measurement
methods that require multiple steps.
Features:
• Simplify Cell Viability Assays: Homogeneous “add-mix-measure”
format dramatically reduces the number of plate handling steps required
for similar assays.
• Use Fewer Cells: Accurately measures cells at numbers below the detection
limits of standard colorimetric and fluorometric assays.
• Get Results Quickly: Record data as soon as 10 minutes after adding
reagent.
• Choose Your Format: Can be used with various multiwell formats. Data
can be recorded by luminometer or CCD camera imaging device.
• Process Plates Consecutively: Luminescent signal is very stable,
allowing batch processing; delivers excellent Z´-factor values.
• Get More Information: Multiplex with other cell-based assays from
Promega.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: For long-term storage, the lyophilized CellTiter-Glo ®
Substrate and CellTiter-Glo ® Buffer should be stored at –20°C. Reconstituted
CellTiter-Glo ® Reagent can be stored at 4°C for 48 hours with ~5% loss of
activity or at 4°C for 4 days with ~20% loss of activity.
Protocol Part#
CellTiter-Glo ® Luminescent Cell Viability Assay Technical Bulletin TB288
CellTiter-Glo ®
Reagent
Mixer
Luminometer
Flow diagram showing preparation and use of CellTiter-Glo ® Reagent.
Luminescence (RLU)
1,800
1,600
1,400
1,200
1,000
800
600
400
200
0
0
10,000 20,000 30,000 40,000 50,000
Cells per Well
For complete and up-to-date product information visit: www.promega.com/catalog
20
10
3170MB
0
0 100 200 300 400
Excellent sensitivity and extended linearity. Serial twofold dilutions of
Jurkat cells were made in RPMI 1640 and 10% PBS in a 96-well plate.
The assay was performed as described in Technical Bulletin #TB288. Values
represent the mean ± S.D. of four replicates for each cell number.
3171MA12_0A