2012 Promega catalogue

Cell Signaling
Anti-ACTIVE ® Caspase-3 pAb
Product Size Cat.# Price ($)
Anti-ACTIVE ® Caspase-3 pAb 50 µl G7481 500.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Anti-ACTIVE ® Caspase-3 pAb is intended for use as a marker
of apoptosis; it specifically stains apoptotic cells without staining nonapoptotic
cells. Includes sufficient antibody to perform 125 immunocytochemical assays
(100μl/assay) at a 1:250 dilution.
Features:
• Immunogen: Peptide derived from the p17 fragment of caspase-3 and
having sequence homology in human, mouse, rat and hamster.
• Antibody Form: Affinity-purified rabbit IgG; supplied in Dulbecco’s PBS.
• Specificity: Specifically recognizes the cleaved active form of caspase-3
in human, rat and mouse.
Storage Conditions: Store at –20°C.
Protocol Part#
Anti-ACTIVE ® Caspase-3 pAb Protocol 9PIG748
Demonstration of Anti-ACTIVE ® Caspase-3 pAb positive cells in
postnatal day 0 (P0) mouse brain paraffin-embedded sections. Panel
A. Three Anti-ACTIVE ® Caspase-3 pAb-positive cells (colored arrows). Panel
B. Corresponding DAPI-stained nuclei. Note the correspondence of Anti-
ACTIVE ® Caspase-3 pAb label with the typical apoptotic, condensed nuclear
morphology in Panel B. Protocols developed and performed at Promega.
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3158TB
Anti-PARP p85 Fragment pAb
Product Size Cat.# Price ($)
Anti-PARP p85 Fragment pAb
For Research Use Only. Not for Use in Diagnostic Procedures.
50 µl G7341 539.00
Description: Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved
in DNA repair, is a substrate for caspase-3 cleavage during apoptosis.
Anti-PARP p85 Fragment pAb is a rabbit polyclonal antibody specific for the
p85 fragment of PARP that results from caspase cleavage of the 116kDa
intact molecule and thus provides an in situ marker for apoptosis. The antibody
is affinity-purified using a peptide that corresponds to a region of the p85
fragment of PARP. The PARP immunogen is a synthetic peptide, gly-val-aspglu-val-ala-lys
(GVDEVAK), representing the N-terminus of the large C-terminal
fragment of human PARP that results from caspase-3 cleavage. Each batch
of antibody is quality assurance tested for use in immunostaining applications
and contains sufficient antibody for 50 immunocytochemical reactions at the
suggested working dilution of 1:100.
Features:
• Immunogen: N-terminal peptide from p85 fragment.
• Antibody Form: Affinity-purified rabbit polyclonal antibody provided in
Dulbecco’s PBS.
• Specificity: Specifically detects PARP p85 fragment in human, rat and bovine
cells and tissues. Does not recognize the 116kDa intact PARP protein.
Storage Conditions: Store at –20°C.
Protocol Part#
Anti-PARP p85 Fragment pAb Technical Bulletin TB273
Anti-PARP p85 Fragment pAb and TUNEL double-labeling of apoptotic
Jurkat cells. Cells were labeled with the Anti-PARP p85 Fragment pAb
(red) and the DeadEnd Fluorometric TUNEL System (Cat.# G3250;
green). The colocalization of cleaved PARP in cells containing TUNEL-positive
nuclei demonstrates that the Anti-PARP p85 Fragment pAb specifically labels
apoptotic cells. Protocols developed and performed at Promega.
2734TA08_9A
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Cell Health Assays
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