2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
32<br />
DeadEnd Fluorometric TUNEL System<br />
Product Size Cat.# Price ($)<br />
DeadEnd Fluorometric TUNEL System 60 reactions G3250 754.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The DeadEnd Fluorometric TUNEL System is a classic TUNEL<br />
Assay designed for the specific detection and quantitation of apoptotic cells<br />
within a cell population. The DeadEnd Fluorometric TUNEL System measures<br />
nuclear DNA fragmentation, an important biochemical hallmark of apoptosis in<br />
many cell types. The system is nonradioactive and provides simple, accurate<br />
and rapid detection of apoptotic cells in situ at the single-cell level or in cell<br />
suspensions. The DeadEnd Fluorometric TUNEL System measures the<br />
fragmented DNA of apoptotic cells by catalytically incorporating fluorescein-<br />
12-dUTP at 3´-OH DNA ends using the enzyme Terminal Deoxynucleotidyl<br />
Transferase (TdT), which forms a polymeric tail using the principle of the TUNEL<br />
(TdT-mediated dUTP Nick-End Labeling) assay. The fluorescein-12-dUTPlabeled<br />
DNA can then be visualized directly by fluorescence microscopy or<br />
quantitated by flow cytometry.<br />
Features:<br />
• Save Money: System provides sufficient reagents for 60 assays of 50μl<br />
each.<br />
• Save Time: Direct incorporation of fluorescent nucleotide reduces number<br />
of incubation steps.<br />
• Choose Sample Type: Use to detect apoptosis in cultured cells and<br />
formalin-fixed, paraffin-embedded tissue sections.<br />
• Convenient: Plastic coverslips provided simplify sample handling.<br />
Storage Conditions: Store at –20°C. Store the Nucleotide Mix protected from<br />
light at –20°C.<br />
Protocol Part#<br />
DeadEnd Fluorometric TUNEL System Technical Bulletin TB235<br />
Neural progenitor cells migrating away from a spherical cluster of<br />
apoptotic cells. The condensed nuclei (green) contain fragmented<br />
DNA, as indicated by fluorescent labeling with the DeadEnd<br />
Fluorometric TUNEL System, in contrast with larger intact<br />
nuclei stained with DAPI (blue). The cells were also processed for<br />
immunocytochemical staining using a primary antibody to βIII Tubulin (Cat.#<br />
G7121) and a Cy ® 3-conjugated secondary antibody where immature processbearing<br />
neurons (red) are distinctly labeled.<br />
2272CA06_8A<br />
Caspase Inhibitor Z-VAD-FMK<br />
Product Size Cat.# Price ($)<br />
Caspase Inhibitor Z-VAD-FMK, 20mM 50 µl G7231 378.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
125 µl G7232 802.00<br />
Description: Z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]fluoromethylketone)<br />
is a cell-permeant pan caspase inhibitor that irreversibly<br />
binds to the catalytic site of caspase proteases and can inhibit induction of<br />
apoptosis. For inhibition of apoptosis, Z-VAD-FMK should be added at the same<br />
time that apoptosis is induced. Z-VAD-FMK is provided at 20mM in DMSO for<br />
convenient addition to cell culture or extracts. The peptide is O-methylated in<br />
the P1 position on aspartic acid, providing enhanced stability and increased cell<br />
permeability. The suggested concentration for use in the anti-Fas mAb-treated<br />
Jurkat cell culture model system is 20μM.<br />
Storage Conditions: Store at –20°C protected from light and moisture.<br />
Caspase Inhibitor Ac-DEVD-CHO<br />
Product Size Conc. Cat.# Price ($)<br />
Caspase Inhibitor Ac-DEVD-CHO 100 µl 10 mM G5961 341.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: Ac-DEVD-CHO is an inhibitor of caspase-3/7 (DEVDase) activity.<br />
The concentration of inhibitor required to inhibit caspase activity must be<br />
determined empirically for each system. Ten micromolar inhibitor is sufficient<br />
to inhibit caspase activity in extracts of apoptotic THP-1 cells. Ac-DEVD-CHO is<br />
supplied as a 10mM solution in DMSO.<br />
Storage Conditions: Store at –20°C protected from light and moisture.<br />
Digitonin<br />
Product Size Cat.# Price ($)<br />
Digitonin<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
40 µl G9441 148.00<br />
Description: Digitonin is a detergent solution useful for permeabilizing cells<br />
and for creating a cytotoxicity chemistry positive control.<br />
Storage Conditions: Store at -20°C protected from light.<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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