2012 Promega catalogue

Cell Signaling
CaspACE Assay System, Colorimetric
Product Size Cat.# Price ($)
CaspACE Assay System, Colorimetric 50 assays G7351 450.00
For Laboratory Use.
100 assays G7220 703.00
Description: The CaspACE Assay System, Colorimetric, provides reagents
for measuring caspase-3 activity. The system includes a colorimetric substrate
and a cell-permeant inhibitor that allow quantitative measurement of caspase-3
(DEVDase) protease activity. The colorimetric substrate (Ac-DEVD-pNA) is
labeled with the chromophore p-nitroaniline (pNA). pNA is released from the
substrate upon cleavage by DEVDase. Free pNA produces a yellow color that is
monitored by a spectrophotometer at 405nm. The amount of yellow color produced
is proportional to the amount of DEVDase activity present in the sample.
The potent, irreversible and cell-permeant pan-caspase inhibitor Z-VAD-FMK
is provided in the CaspACE Assay System, Colorimetric. The addition of the
Z-VAD-FMK Inhibitor prior to the induction of apoptosis in cell culture inhibits
the activation of the caspase cascade, including caspase-3.
Features:
• Timely: Measures an early indicator of apoptosis.
• Quantitative or Qualitative: Determine total caspase-3 activity or screen
for inducers or inhibitors of caspase activity.
• Versatile: May be used with purified enzyme preparations, cell extracts or
tissue lysates.
Storage Conditions: Store at –20°C. Store substrates and inhibitors in
aliquots at –20°C away from light and moisture.
Protocol Part#
CaspACE Assay System, Colorimetric Technical Bulletin TB270
CaspACE FITC-VAD-FMK In Situ Marker
Product Size Cat.# Price ($)
CaspACE FITC-VAD-FMK In Situ Marker 50 µl G7461 343.00
For Research Use Only. Not for Use in Diagnostic Procedures.
125 µl G7462 717.00
Description: CaspACE FITC-VAD-FMK In Situ Marker is a fluorescent
analog of the pan caspase inhibitor Z-VAD-FMK (carbobenzoxy-valyl-alanylaspartyl-[O-methyl]-fluoromethylketone).
The fluorescein isothiocyanate (FITC)
group has been substituted for the carbobenzoxy (Z) N-terminal blocking group
to create the fluorescent apoptosis marker. This structure allows delivery of the
inhibitor into the cell where it irreversibly binds to activated caspases. The FITC
label enables a single-reagent addition to assay for caspase activity in situ. The
FITC-VAD-FMK is supplied as a 5mM solution in DMSO and is intended for in
situ monitoring of caspase activity by fluorescence detection. The suggested
concentration for use in anti-Fas-treated Jurkat cell culture is 10μM.
Features:
• Simplify Your Protocol: Add FITC-VAD-FMK, incubate, wash and view
fluorescence. No preparation of cell extracts or long incubations.
• Use a Variety of Detection Methods: Detect apoptotic cells by microscopy
or flow cytometry; combine with other markers to assess cell populations
or determine apoptotic frequency within a population; adaptable to
high-throughput applications.
• Get Reliable Results: Synthesized peptide provides consistent results
from every batch, unlike Annexin V, which can be highly variable between
batches.
• Use With Live Cells: Easily moves in and out of cells and remains
anchored inside cultured apoptotic cells.
Storage Conditions: Store at –20°C protected from light and moisture.
Protocol Part#
CaspACE FITC-VAD-FMK In Situ Marker Protocol 9PIG746
For complete and up-to-date product information visit: www.promega.com/catalog
DeadEnd Colorimetric TUNEL System
Product Size Cat.# Price ($)
DeadEnd Colorimetric TUNEL System 20 reactions G7360 444.00
40 reactions G7130 698.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The DeadEnd Colorimetric TUNEL System is a modified TUNEL
Assay designed to provide simple, accurate and rapid detection of apoptotic
cells in situ at the single-cell level. The DeadEnd Colorimetric TUNEL System
measures nuclear DNA fragmentation, an important biochemical indicator of
apoptosis. The system can be used to assay apoptotic cell death in tissue sections
and cultured cells. The DeadEnd Colorimetric TUNEL System end-labels
the fragmented DNA of apoptotic cells using a modified TUNEL (TdT-mediated
dUTP Nick-End Labeling) assay. Biotinylated nucleotide is incorporated at the
3´-OH DNA ends using the enzyme Terminal Deoxynucleotidyl Transferase (TdT).
Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound
to these biotinylated nucleotides, which are detected using the peroxidase substrate,
hydrogen peroxide, and the stable chromogen, diaminobenzidine (DAB).
Using this procedure, apoptotic nuclei are stained dark brown.
Features:
• Assay Cells or Tissue: Detect apoptosis in thick tissue sections or assess
cell morphology.
• Simplify: Includes DAB substrate and H 2O 2 for color detection and plastic
coverslips that simplify sample handling.
• Proven Applications: Vibratome ® sections of neuronal tissue, Jurkat
cells, HL-60 cells.
Storage Conditions: Store the Equilibration Buffer, TdT Enzyme, Biotinylated
Nucleotide Mix and Proteinase K at –20°C. Store the Streptavidin HRP, DAB
20X Chromogen, DAB Substrate 20X Buffer and Hydrogen Peroxide 20X at
4°C. Store the SSC 20X and Plastic Coverslips at room temperature.
Protocol Part#
DeadEnd Colorimetric TUNEL System Technical Bulletin TB199
31
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Cell Health Assays
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