2012 Promega catalogue

Cell Signaling
Caspase-Glo ® 9 Assay Systems
Product Size Cat.# Price ($)
Caspase-Glo ® 9 Assay 2.5 ml G8210 180.00
For Laboratory Use.
10 ml G8211 602.00
100 ml G8212 Pls. Enq.
Description: The homogeneous Caspase-Glo ® 9 Assay is a measures caspase-9
activity using a proluminogenic caspase-9 substrate in a buffer system
optimized for caspase activity, luciferase activity and cell lysis. The addition of
a single Caspase-Glo ® 9 Reagent in an “add-mix-read” format results in cell
lysis, followed by caspase cleavage of the substrate and generation of a “glowtype”
luminescent signal. The signal generated is proportional to the amount of
caspase activity present. The Caspase-Glo ® Reagent relies on the properties
of a proprietary thermostable luciferase (Ultra-Glo Recombinant Luciferase),
which generates the stable “glow-type” luminescent signal and improves
performance across a wide range of assay conditions.
The system now includes a separate vial of MG-132 protease inhibitor, which
may be used to reduce background, thus improving the performance of the
Caspase-Glo ® 9 Assay in cell-based applications.
For complete and up-to-date product information visit: www.promega.com/catalog
Features:
• Simplify Apoptosis or Caspase Detection: The homogeneous “addmix-read”
protocol makes the assay easy to automate; simply add an equal
volume of reagent to sample volume.
• Use Less Enzyme: Excellent signal-to-noise ratios and superior sensitivity
allow assays to be performed in 96- or 384-well formats.
• Decrease Assay Time: No sample preparation or manipulation required,
and no extended incubation times are necessary as with fluorescent-based
assays. Maximum sensitivity is achieved in as little as 0.5–1 hour.
• Rely on a Performance-Tested Assay: The assay delivers excellent Z´
factors in cell and purified enzyme models.
• Get More Information: Multiplex with other cell-based assays from
Promega.
• Experience Improved Caspase-9 Selectivity: Caspase-Glo ® 9 Assay
uses a luminogenic substrate containing the LEHD sequence, which has
been shown to be selective for caspase-9. The assay includes an optional
proteasome inhibitor (MG-132), which when added to the Caspase-Glo ® 9
Reagent significantly reduces nonspecific background in cell-based assays.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C protected from light.
Protocol Part#
Caspase-Glo ® 9 Assay Technical Bulletin TB333
29
2
Cell Health Assays
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