2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Cell Signaling<br />
pUC/M13 Sequencing Primers<br />
Product Size Conc. Cat.# Price ($)<br />
pUC/M13 Primer, Forward (17mer) 2 µg 10 µg/ml Q5391 134.00<br />
pUC/M13 Primer, Reverse (17mer) 2 µg 10 µg/ml Q5401 134.00<br />
pUC/M13 Primer, Reverse (22mer) 2 µg 10 µg/ml Q5421 134.00<br />
pUC/M13 Primer, Forward (24mer) 2 µg 10 µg/ml Q5601 134.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pUC/M13 Primers are designed for sequencing inserts<br />
cloned into the M13 vectors and pUC plasmids developed by Messing. These<br />
primers also can be used for sequencing other lacZ-containing plasmids<br />
such as the pGEM ® -Z and pGEM ® -Zf Vectors. The primers are purified by gel<br />
electrophoresis or HPLC.<br />
Primer Sequences<br />
• Forward (17mer): 5´-d(GTTTTCCCAGTCACGAC)-3´<br />
• Reverse (17mer): 5´-d(CAGGAAACAGCTATGAC)-3´<br />
• Reverse (22mer): 5´-d(TCACACAGGAAACAGCTATGAC)-3´<br />
• Forward (24mer): 5´-d(CGCCAGGGTTTTCCCAGTCACGAC)-3´<br />
Storage Conditions: Store at –20°C. The primers are supplied in sterile<br />
water.<br />
T-Vectors<br />
pGEM ® -T Vector Systems<br />
Product Size Cat.# Price ($)<br />
pGEM ® -T Vector System I 20 reactions A3600 268.00<br />
pGEM ® -T Vector System II 20 reactions A3610 448.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
For additional information see page 219.<br />
XmnI 1994<br />
ScaI<br />
1875<br />
Amp r<br />
pGEM ® -T<br />
Vector<br />
(3000bp)<br />
ori<br />
NaeI<br />
2692<br />
f1 ori<br />
T7<br />
1 start<br />
ApaI 14<br />
AatII 20<br />
SphI 26<br />
BstZI 31<br />
lacZ<br />
T T<br />
NcoI<br />
SacII<br />
37<br />
46<br />
SpeI 55<br />
NotI 62<br />
BstZI 62<br />
PstI 73<br />
SalI 75<br />
NdeI 82<br />
SacI 94<br />
BstXI 103<br />
NsiI 112<br />
SP6<br />
126<br />
➞<br />
➞<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
0356VA04_3A<br />
pGEM ® -T Easy Vector Systems<br />
Product Size Cat.# Price ($)<br />
pGEM ® -T Easy Vector System I 20 reactions A1360 282.00<br />
pGEM ® -T Easy Vector System II 20 reactions A1380 463.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
For additional information see page 219.<br />
XmnI 2009<br />
ScaI 1890<br />
Amp r<br />
pGEM ® -T Easy<br />
Vector<br />
(3015bp)<br />
ori<br />
f1 ori<br />
lacZ<br />
NaeI 2707<br />
T T<br />
T7<br />
ApaI<br />
AatII<br />
SphI<br />
BstZI<br />
NcoI<br />
BstZI<br />
NotI<br />
SacII<br />
EcoRI<br />
➞<br />
SpeI<br />
EcoRI<br />
NotI<br />
BstZI<br />
PstI<br />
SalI<br />
NdeI<br />
SacI<br />
BstXI<br />
NsiI<br />
➞<br />
SP6<br />
1 start<br />
14<br />
20<br />
26<br />
31<br />
37<br />
43<br />
43<br />
49<br />
52<br />
64<br />
70<br />
77<br />
77<br />
88<br />
90<br />
97<br />
109<br />
118<br />
127<br />
141<br />
pTargeT Mammalian Expression Vector<br />
System<br />
Product<br />
pTargeT Mammalian Expression<br />
Size Cat.# Price ($)<br />
Vector System 20 reactions A1410 569.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
For additional information see page 220.<br />
Ampr<br />
ori<br />
Synthetic<br />
poly(A)<br />
Neo<br />
BglII 5665<br />
CMV<br />
Enhancer/Promoter<br />
pTARGET<br />
Vector<br />
(5670bp)<br />
Intron<br />
fl ori<br />
SV40 Enhancer/<br />
EarlyPromoter<br />
SgfI 664<br />
SV40 Late<br />
poly (A)<br />
I-PpoI<br />
851<br />
T<br />
T<br />
lacZ<br />
T7<br />
EcoRI<br />
BamHI<br />
NheI<br />
XhoI<br />
MluI<br />
lacZ<br />
➞<br />
T overhangs<br />
SmaI<br />
KpnI<br />
SalI<br />
AccI<br />
NotI<br />
EcoRI<br />
1250<br />
1256<br />
1264<br />
1270<br />
1276<br />
1293<br />
1301<br />
1303<br />
1304<br />
1311<br />
1318<br />
1473VA05_6A<br />
1505VA07_6A<br />
309<br />
18<br />
Vectors<br />
Section<br />
Contents<br />
Table of<br />
Contents
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