2012 Promega catalogue

Cell Signaling
Caspase-Glo ® 2 Assay Systems
Product Size Cat.# Price ($)
Caspase-Glo ® 2 Assay 10 ml G0940 648.00
For Laboratory Use.
50 ml G0941 Pls. Enq.
Description: The luminescent Caspase-Glo ® 2 Assay measures caspase-2
activity in a homogeneous format. Caspase-2 is a member of the cysteine
aspartic acid-specific protease family. The Caspase-Glo ® 2 Assay provides a
luminogenic substrate (Z-VDVAD-aminoluciferin) in a reagent optimized for
caspase-2 and luciferase activity. A single reagent is added to test samples,
resulting in caspase cleavage of the substrate and generation of a glow-type
luminescent signal produced by luciferase. Luminescence is proportional to the
amount of caspase activity present. The assay system may be used with
purified enzyme preparations and is ideal for automated high-throughput
screening of inhibitors.
Features:
• Broad Dynamic Range: The assay is linear over four logs of caspase-2
concentration and can detect caspase-2 activity at concentrations as low
as 0.2mU/ml.
• High-Quality Assay: The assay demonstrates an excellent Z´-factor value
of 0.85 in 384-well plates using 0.05U/ml of enzyme.
• Increased Accuracy: The superior sensitivity over fluorescence-based
caspase assays allows inhibitor studies to be performed below the K m.
• Batch Processing Capability: The coupled-enzyme, homogeneous
format results in a continuous signal, providing excellent stability and
allowing plates to be read over an extended period of time. Luminometers
with reagent injectors are not required.
Storage Conditions: Store at –20°C.
Protocol Part#
Caspase-Glo ® 2 Assay Technical Bulletin TB365
Caspase-Glo ®
Reagent
Luminometer
Add equal
volume of
reagent to
samples.
Mix. Incubate.
Measure
luminescence.
Schematic diagram of the Caspase-Glo ® Assay protocol.
For complete and up-to-date product information visit: www.promega.com/catalog
4689MB
Caspase-Glo ® 3/7 Assay Systems
Product Size Cat.# Price ($)
Caspase-Glo ® 3/7 Assay 2.5 ml G8090 192.00
For Laboratory Use.
10 ml G8091 654.00
10 × 10 ml G8093 Pls. Enq.
100 ml G8092 Pls. Enq.
Description: The homogeneous Caspase-Glo ® 3/7 Assay measures
caspase-3/7 activities using a proluminescent caspase-3/7 DEVD-aminoluciferin
substrate and a proprietary thermostable luciferase in a reagent
optimized for caspase-3/7 activity, luciferase activity and cell lysis. Adding the
single Caspase-Glo ® 3/7 Reagent results in cell lysis, followed by caspase
cleavage of the substrate. This liberates free aminoluciferin, which is consumed
by the luciferase, generating a “glow-type” luminescent signal. The signal is
proportional to caspase-3/7 activity. The stabilized luciferase and proprietary
buffer system improve assay performance across a wide range of assay conditions,
and the assay is less likely to be affected by compound interference than
fluorescent- or colorimetric-based assays.
Features:
• Simplify Apoptosis or Caspase Detection: The “add-mix-measure”
protocol makes the assay easy to automate.
• Use Less Sample: Excellent signal-to-noise ratios and superior sensitivity
allow assays to be performed in 96- or 384-well formats.
• Decrease Assay Time: No sample preparation or manipulation required,
and no extended incubation times are necessary. Maximum sensitivity
achieved in as little as 0.25–1 hour.
• Rely on a Performance-Tested Assay: The assay delivers excellent Z´factor
values in cell and purified enzyme models.
• Process Plates in Batch Mode: The extended-glow signal allows the
plates to be read over a 3-hour time period; no injectors required.
• Get More Information: Multiplex with other assays from Promega.
• Automate This Assay: Validated automated methods available at:
www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
Caspase-Glo ® 3/7 Assay Technical Bulletin TB323
Luminescence (RLU)
Background Subtracted
160,000 12,000
140,000
120,000
100,000
80,000
60,000
40,000
20,000
0
10,000
8,000
6,000
4,000
2,000
0 0 200 400 600 800
0
1,000
Anti-Fas-Treated Cells
Untreated Cells
2,000
3,000
4,000
5,000
6,000
7,000
8,000
9,000
10,000
Cell # 4098MB05_3A
The Caspase-Glo ® 3/7 Assay output is linear over a broad range of
cell numbers. Jurkat cells were treated with anti-Fas mAb for to
induce apoptosis or were left untreated.
27
2
Cell Health Assays
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