2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
In Vitro Translation Specialty Vectors
302
In Vitro Translation Specialty Vectors
Product Size Cat.# Price ($)
pF3A WG (BYDV) Flexi ® Vector 20 µg L5671 465.00
pF3K WG (BYDV) Flexi ® Vector 20 µg L5681 465.00
pF25A ICE T7 Flexi ® Vector 20 µg L1061 478.00
pF25K ICE T7 Flexi ® Vector 20 µg L1081 478.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These Flexi ® vectors are designed with special sequences for
maximal cell-free protein expression in a specific system. The pF3A/K WG
vectors were designed for use with Wheat Germ extracts and contain
sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus,
upstream and downstream of the protein coding region of interest. The BYDV
elements interact with each other, form a closed loop and act synergistically to
stimulate translation in wheat germ extracts, bypassing mRNA cap and
polyadenylation dependencies. The pF25A/K ICE Vectors were designed for use
with Insect Cell Extracts and contain untranslated region (UTR) sequences at
the 5´ and 3´ ends of the gene coding region to enhance translation efficiency.
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background
colonies without inserts during the subcloning procedure. Using the Flexi ® Vector
Cloning System replaces the barnase gene with your insert. These vectors,
as purchased, cannot be cultured in normal laboratory strains of E. coli without
an insert.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
pTnT Vector
Product Size Cat.# Price ($)
pTnT Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg L5610 341.00
Description: The pTnT Vector is designed for the convenient in vitro
expression of cloned genes. Both SP6 and T7 polymerase promoters lie in
tandem adjacent to the multiple cloning site. This permits gene expression from
either an SP6- or T7-based coupled in vitro transcription/translation system.
The presence of RNA phage promoters also allows for the highly efficient
synthesis of RNA in vitro. The pTnT Vector also contains a 5´ β-globin leader
sequence and synthetic poly(A) 30 tail, both of which have been shown to
enhance expression of certain genes.
Features:
• Flexible: The vector contains tandem SP6 and T7 phage promoters
allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites
for cloning.
Storage Conditions: Store at –20°C.
Protocol Part#
pTnT Vector Technical Bulletin TB304
pCMVTnT Vector
Product Size Cat.# Price ($)
pCMVTnT Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg L5620 391.00
Description: The pCMVTnT Vector is designed for the convenient
expression of cloned genes using both in vivo and in vitro expression systems.
Both SP6 and T7 polymerase promoters lie in tandem adjacent to the multiple
cloning site. This allows for gene expression from either an SP6- or T7-based
coupled in vitro transcription/translation system. The presence of RNA phage
promoters also allows for the highly efficient synthesis of RNA in vitro. The
pCMVTnT Vector also contains a 5´ β-globin leader sequence that has
been referenced for enhanced expression of certain genes in vitro. For in vivo
expression, the vector contains a CMV enhancer/promoter region, which allows
strong constitutive expression in many cell types. A β-globin/IgG chimeric
intron is located downstream from the enhancer/promoter region. The late
SV40 polyadenylation site is located downstream of the multiple cloning site.
Features:
• In Vivo Expression: The CMV enhancer/promoter region allows strong
constitutive expression in many cell types.
• Flexible: The vector contains tandem SP6 and T7 phage promoters
allowing use in the appropriate in vitro translation or transcription system.
• Convenient: Multiple cloning site provides a selection of restriction sites
for cloning.
Storage Conditions: Store at –20°C.
Protocol Part#
pCMVTnT Vector TB305
Chroma-Luc Vectors
Product Size Cat.# Price ($)
pCBR-Basic Vector 20 µg E1411 472.00
pCBR-Control Vector 20 µg E1421 472.00
pCBG68-Basic Vector 20 µg E1431 472.00
pCBG68-Control Vector 20 µg E1441 472.00
pCBG99-Basic Vector 20 µg E1451 472.00
pCBG99-Control Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg E1461 472.00
For additional information see page 272.
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