2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 300 pAdVAntage Vector Product Size Cat.# Price ($) pAdVAntage Vector 20 µg E1711 167.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing translation initiation. Transfection of mammalian cells with an expression vector often results in suboptimal expression of the protein of interest. Double-stranded RNA (dsRNA) generated during transfection is thought to activate the dsRNA-activated inhibitor (DAI), one of several enzymes involved in the host cell’s antiviral defense system. DAI phosphorylates the translation initiation factor eIF-2, halting translation and therefore protein production. However, DAI translation inhibition can be overcome with the adenoviral Virus Associated I RNA (VAI RNA) produced by RNA polymerase III following co-transfection with the pAdVAntage Vector. The VAI RNA binds to DAI, preventing its activation, thereby allowing translation and protein expression. Features: • Increased Expression: Co-transfection of pAdVAntage Vector with luciferase constructs showed at least a tenfold increase in luciferase expression in 293 and HeLa cell lines over transfections performed with the construct DNA alone. • Flexible: Can be used in a variety of cell lines. Storage Conditions: Store at –20°C. Protocol Part# pAdVAntage Vector Technical Bulletin TB207 pSI Mammalian Expression Vector Product Size Cat.# Price ($) pSI Mammalian Expression Vector For Research Use Only. Not for Use in Diagnostic Procedures. 20 µg E1721 423.00 Description: The pSI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the pCI and pSI Mammalian Expression Vectors is the enhancer/ promoter region controlling the expression of the inserted gene. The pSI Expression Vector contains the simian virus 40 (SV40) enhancer and early promoter region. This vector can be used for both transient and stable expression of genes. For stable expression, the pSI Vector must be co-transfected with an expression vector containing a selectable gene for mammalian cells. Features: • Strong, Constitutive Expression: The pSI Vector’s SV40 enhancer/ promoter region allows strong, constitutive expression in most cell lines. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. • Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal. • Convenient: Multiple cloning sites exist for easy insertion of cDNA. • Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication. Storage Conditions: Store at –20°C. Protocol Part# pCI and pSI Mammalian Expression Vectors Technical Bulletin TB206 Amp r ori pSI Vector (3632bp) Intron T7 NheI XhoI EcoRI MluI XbaI SalI AccI SmaI BstZI NotI ➞ For complete and up-to-date product information visit: www.promega.com/catalog f1 ori BglII SV40 Enhancer/ Early Promoter SV40 Late poly(A) Bst98I PstI Bst98I BamHI 678 684 689 695 707 713 714 720 724 724 0684VA06_4B
Cell Signaling pCI Mammalian Expression Vector Product Size Cat.# Price ($) pCI Mammalian Expression Vector 20 µg E1731 423.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The pCI Mammalian Expression Vector promotes constitutive expression of cloned DNA inserts in mammalian cells. The major difference between the pCI and pSI Mammalian Expression Vectors is the enhancer/ promoter region controlling the expression of the inserted gene. The pCI Expression Vector contains the human cytomegalovirus (CMV) major immediate-early gene enhancer/promoter region. This vector can be used for both transient and stable expression of genes. For stable expression, the pCI Vector must be co-transfected with an expression vector containing a selectable gene for mammalian cells. Features: • Strong, Constitutive Expression: The pCI Vector’s CMV enhancer/ promoter region enables strong, constitutive expression in many cell types. A β-globin/IgG chimeric intron located downstream of the enhancer/ promoter region can further increase expression. • Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal. • Convenient: Multiple cloning sites exist for easy insertion of cDNA. • Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication. Storage Conditions: Store at –20°C. Protocol Part# pCI and pSI Mammalian Expression Vectors Technical Bulletin TB206 Amp r BglII ori CMV I. E. Enhancer/Promoter pCI Vector (4006bp) f1 ori Intron SV40 Late poly(A) Bst98I PstI Bst98I BamHI T7 NheI XhoI EcoRI MluI KpnI XbaI SalI AccI SmaI BstZI NotI ➞ 1052 1058 1063 1069 1079 1081 1087 1088 1094 1098 1098 For complete and up-to-date product information visit: www.promega.com/catalog 0685VA06_4A pCI-neo Mammalian Expression Vector Product Size Cat.# Price ($) pCI-neo Mammalian Expression Vector For Research Use Only. Not for Use in Diagnostic Procedures. 20 µg E1841 455.00 Description: The pCI-neo Mammalian Expression Vector carries the human cytomegalovirus (CMV) immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in mammalian cells. This vector also contains the neomycin phosphotransferase gene, a selectable marker for mammalian cells. The pCI-neo Vector can be used for transient or stable expression by selecting transfected cells with the antibiotic G-418. Features: • Strong, Constitutive Expression: The human cytomegalovirus (CMV) immediate-early enhancer/promoter region produces strong, constitutive expression. A β-globin/IgG chimeric intron located downstream from the enhancer/promoter region can further increase expression. The vector is maintained as an episome in cells expressing the SV40 large T antigen, leading to even higher levels of expression. • Transient or Stable Expression: The neomycin phosphotransferase gene allows selection of stable transfected cells. • Increased Steady-State mRNA Levels: The late SV40 polyadenylation signal increases the steady-state level of RNA approximately fivefold more than the early SV40 polyadenylation signal. • Convenient: Multiple cloning sites exist for easy insertion of cDNA. • Versatile: Synthesize transcripts in vitro using the T7 RNA polymerase promoter or generate single-stranded DNA in E. coli using the f1 origin of replication. Storage Conditions: Store at –20°C. Protocol Part# pCI-neo Mammalian Expression Vector Technical Bulletin TB215 BamHI Amp r Synthetic poly(A) BglII ori CMV I.E. Enhancer/Promoter pCI-neo Vector (5472bp) Intron SV40 Enhancer/ Early Promoter neo f1 ori SgfI SV40 Late poly(A) I-PpoI T7 NheI XhoI EcoRI MluI XbaI SalI AccI SmaI NotI T3 1085 1091 1096 1102 1114 1120 1121 1127 1131 0914VA01_5A 301 18 Vectors Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Inducible T7-Driven
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Cell Signaling ProtoBlot ® II AP S
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Cell Signaling HaloTag ® Protein P
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Cell Signaling HaloTag ® Mammalian
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Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291 and 292: Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294: Cell Signaling RNA Interference Gen
Page 295 and 296: Cell Signaling 17 Stem Cell Researc
Page 297 and 298: Cell Signaling Cell ID System Produ
Page 299 and 300: Cell Signaling StemElite Human Panc
Page 301 and 302: Cell Signaling 18 Vectors For compl
Page 303: Cell Signaling HaloTag ® Fusion (N
Page 307 and 308: Cell Signaling Reporter Vectors pGL
Page 309 and 310: Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312: Cell Signaling pGEM ® and pSP Subc
Page 313 and 314: Cell Signaling pUC/M13 Sequencing P
Page 315 and 316: Index: A-Z 19 Index For complete an
Page 317 and 318: BssHII.............................
Page 319 and 320: In vitro Transcription Systems Rela
Page 321 and 322: QuantiFluor dsDNA System ..........
Page 323 and 324: Cat# Product Size Page Price($) A10
Page 325 and 326: Index by Catalog Number Cat# Produc
Page 327 and 328: Cat# Product Size Page Price($) C84
Page 329 and 330: Cat# Product Size Page Price($) E13
Page 331 and 332: Cat# Product Size Page Price($) E61
Page 333 and 334: Cat# Product Size Page Price($) G13
Page 335 and 336: Cat# Product Size Page Price($) G77
Page 337 and 338: Cat# Product Size Page Price($) L50
Page 339 and 340: Cat# Product Size Page Price($) P16
Page 341 and 342: Cat# Product Size Page Price($) R99
Page 343 and 344: Cat# Product Size Page Price($) V42
Page 345 and 346: Cat# Product Size Page Price($) W39
Page 347 and 348: personal DETECTION GloMax Integrat
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2012 Promega catalogue

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