2012 Promega catalogue

Cell Signaling
StemElite Human Pancreatic-Associated
Transcripts
Product Size Cat.# Price ($)
StemElite HNF4A/GAPDH Primer Pair (20X) 100 µl B1301 134.00
StemElite HNF1B/GAPDH Primer Pair (20X) 100 µl B1311 134.00
StemElite PDX1/GAPDH Primer Pair (20X) 100 µl B1321 134.00
StemElite INS/GAPDH Primer Pair (20X) 100 µl B1331 134.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Pluripotential stem cells can give rise to differentiated cells and
tissues for all three embryonic germ layers. HNF4A, HNF1B, PDX1 and INS are
mesodermal markers associated with differentiation of pancreatic cells.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplex
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all transcripts in the same well.
Storage Conditions: Store at –20°C.
StemElite Differentiation-Associated
Transcripts
Product Size Cat.# Price ($)
StemElite FOXA2/GAPDH Primer Pair (20X) 100 µl B1341 134.00
StemElite SOX17/GAPDH Primer Pair (20X) 100 µl B1351 134.00
StemElite GATA6/GAPDH Primer Pair (20X) 100 µl B1361 134.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Pluripotential stem cells can give rise to differentiated cells and
tissues for all three embryonic germ layers. FOXA2, SOX17 and GATA6 are
nonspecific differentiation markers.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplex
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(GAPDH).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all transcripts in the same well.
Storage Conditions: Store at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog
StemElite Mouse Pluripotent Transcripts
Product Size Cat.# Price ($)
StemElite Mus-Nanog/Actb Primer Pair (20X) 100 µl B1371 134.00
StemElite Mus-Sox2/Actb Primer Pair (20X) 100 µl B1381 134.00
StemElite Mus-Pou5f1/Actb Primer Pair (20X) 100 µl B1391 134.00
StemElite Mus-Lin28/Actb Primer Pair (20X) 100 µl B1401 134.00
StemElite Mus-Klf4/Actb Primer Pair (20X) 100 µl B1411 134.00
StemElite Mus-Myc/Actb Primer Pair (20X) 100 µl B1421 134.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Mus-Nanog, Mus-Sox2, Mus-Pou5f1, Mus-Lin28, Mus-Klf4 and
Mus-Myc are functionally associated with maintenance of the undifferentiated
mouse embryonic stem cell.
Features:
• Achieve sensitive real-time detection and relative quantification of RNA
expression levels for monitoring stem cell differentiation in a multiplex
amplification.
• Quantitatively amplify a two-color duplex.
• Amplify in a single tube the transcript of interest and reference transcript
(Actb).
• Reduce the number of reactions required to measure multiple transcripts.
• Improve experimental data quality by measuring all transcripts in the same
well.
Storage Conditions: Store at –20°C.
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Stem Cell Research
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