2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
294<br />
PowerPlex ® 16 HS System<br />
Product Size Cat.# Price ($)<br />
PowerPlex ® 16 HS System 100 reactions DC2101 Pls. Enq.<br />
400 reactions DC2100 Pls. Enq.<br />
DG1071, DW0991 For Laboratory Use. DC2101, DC2100, DD1001 Not For Medical Diagnostic<br />
Use.<br />
For additional information see page 154.<br />
Stemness Assessment<br />
StemElite Gene Expression System<br />
Product<br />
StemElite Gene<br />
Size Cat.# Price ($)<br />
Expression System 100 qPCR reactions B1001 258.00<br />
StemElite Gene<br />
Expression System Plus<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
100 qPCR reactions +<br />
50 RT reactions B1002 423.00<br />
Description: The StemElite Gene Expression System is a novel real-time<br />
quantitative PCR (qPCR) system for detection and relative quantification of RNA<br />
expression levels associated with the differentiation state or ‘potency’ of cells.<br />
The StemElite Gene Expression System is optimized to quantitatively amplify<br />
a two-color duplex, enabling the user to amplify a transcript of interest as well<br />
as a reference gene in a single reaction.<br />
Features:<br />
• Achieve sensitive real-time detection and relative quantification of RNA<br />
expression levels for monitoring stem cell differentiation in a multiplex<br />
amplification.<br />
• Quantitatively amplify a two-color duplex.<br />
• Amplify in a single tube the transcript of interest and reference transcript<br />
(GAPDH or Actb).<br />
• Reduce the number of reactions required to measure multiple transcripts.<br />
• Improve experimental data quality by measuring all transcripts in the same<br />
well.<br />
Storage Conditions: Store at –20°C.<br />
Protocol Part#<br />
StemElite Gene Expression System Technical Manual TM323<br />
StemElite Human Pluripotent Transcripts<br />
Product Size Cat.# Price ($)<br />
StemElite NANOG/GAPDH Primer Pair (20X) 100 µl B1011 134.00<br />
StemElite SOX2/GAPDH Primer Pair (20X) 100 µl B1021 134.00<br />
StemElite POU5F1/GAPDH Primer Pair (20X) 100 µl B1031 134.00<br />
StemElite LIN28/GAPDH Primer Pair (20X) 100 µl B1041 134.00<br />
StemElite KLF4/GAPDH Primer Pair (20X) 100 µl B1051 134.00<br />
StemElite MYC/GAPDH Primer Pair (20X)<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
100 µl B1061 134.00<br />
Description: NANOG, SOX2, POU5F1, LIN28, KLF4 and MYC are functionally<br />
associated with maintenance of the undifferentiated human embryonic stem cell.<br />
Features:<br />
• Achieve sensitive real-time detection and relative quantification of RNA<br />
expression levels for monitoring stem cell differentiation in a multiplex<br />
amplification.<br />
• Quantitatively amplify a two-color duplex.<br />
• Amplify in a single tube the transcript of interest and reference transcript<br />
(GAPDH).<br />
• Reduce the number of reactions required to measure multiple transcripts.<br />
• Improve experimental data quality by measuring all transcripts in the same well.<br />
Storage Conditions: Store at –20°C.<br />
StemElite Human Heart-Associated<br />
Transcripts<br />
Product Size Cat.# Price ($)<br />
StemElite NPPA/GAPDH Primer Pair (20X) 100 µl B1071 134.00<br />
StemElite MYL7/GAPDH Primer Pair (20X) 100 µl B1081 134.00<br />
StemElite MYL2/GAPDH Primer Pair (20X) 100 µl B1091 134.00<br />
StemElite MYH6/GAPDH Primer Pair (20X) 100 µl B1101 134.00<br />
StemElite MYH7/GAPDH Primer Pair (20X) 100 µl B1111 134.00<br />
StemElite NKX2-5/GAPDH Primer Pair (20X) 100 µl B1121 134.00<br />
StemElite TNNT2/GAPDH Primer Pair (20X) 100 µl B1131 134.00<br />
StemElite TNNI3/GAPDH Primer Pair (20X) 100 µl B1141 134.00<br />
StemElite MEF2C/GAPDH Primer Pair (20X) 100 µl B1151 134.00<br />
StemElite PLN/GAPDH Primer Pair (20X) 100 µl B1161 134.00<br />
StemElite GATA4/GAPDH Primer Pair (20X) 100 µl B1171 134.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: Pluripotential stem cells can give rise to differentiated cells and<br />
tissues for all three embryonic germ layers. NPPA, MYL7, MYL2, MYH6, MYH7,<br />
NKX2-5, TNNT2, TNNI3, MEF2C, PLN and GATA4 are mesodermal markers<br />
associated with differentiation of cardiac muscle.<br />
Features:<br />
• Achieve sensitive real-time detection and relative quantification of RNA<br />
expression levels for monitoring stem cell differentiation in a multiplex<br />
amplification.<br />
• Quantitatively amplify a two-color duplex.<br />
• Amplify in a single tube the transcript of interest and reference transcript<br />
(GAPDH).<br />
• Reduce the number of reactions required to measure multiple transcripts.<br />
• Improve experimental data quality by measuring all transcripts in the same well.<br />
Storage Conditions: Store at –20°C.<br />
For complete and up-to-date product information visit: www.promega.com/catalog
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