2012 Promega catalogue

Cell Signaling
Cell ID System
Product Size Cat.# Price ($)
Cell ID System 50 reactions G9500 Pls. Enq.
Available Separately
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
Internal Lane Standard 600 150 µl DG1071 354.00
DW0991, DG1071 For Laboratory Use. G9500 For Research Use Only. Not for Use in Diagnostic
Procedures.
Description: The Cell ID System provides the reagents required for successful
and simple identification and authentication of human cell lines and
detection of intra-species cell line cross-contamination. The Cell ID System
uses short tandem repeat (STR) analysis of specific loci in the human genome
through co-amplification and three-color detection of ten loci (nine STR loci and
Amelogenin for gender identification), including D21S11, TH01, TPOX, vWA,
Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818. All ten loci
are amplified simultaneously in a single tube. The Cell ID System includes a
hot-start Taq DNA polymerase in an enzyme mix for convenient room-temperature
reaction assembly. Alleles, along with the allelic ladder and an internal lane
standard that assists in assigning sizes for each of the loci, are resolved by
capillary electrophoresis in a single injection, and CE data are analyzed using
allele-calling software to generate a genetic profile of the cell line. These loci
collectively provide a genetic profile with a random match probability of 1 in
2.92 × 10 9 .
The Cell ID System is designed specifically for use with the Applied Biosystems
3130 and 3130xl Genetic Analyzers, ABI PRISM ® 3100 and 3100-Avant
Genetic Analyzers and ABI PRISM ® 310 Genetic Analyzer.
Dye matrix generation is required on initial setup and is performed using the
PowerPlex ® Matrix Standards, 310 (Cat.# DG4640), or PowerPlex ® Matrix
Standards, 3100/3130 (Cat.# DG4650).
For complete and up-to-date product information visit: www.promega.com/catalog
Features:
• Improved “Gold Standard” STR Analysis: The Cell ID System
includes all loci used in the original PowerPlex ® 1.2 System plus the
D21S11 locus for improved power of discrimination.
• Simplified Data Interpretation: The Cell ID System allows coamplification
of 10 loci to accurately discriminate between human cell
lines without using a full forensic profile and includes allelic ladders for all
10 loci to simplify data interpretation.
• Simplified Amplification Setup: Inclusion of a hot-start Taq DNA
polymerase allows room-temperature reaction assembly.
• Internal Lane Standard 600 Included: This marker offers the greatest
precision available for DNA typing. It is designed for use in each capillary
injection to increase the precision of your analyses.
• Automatic Assignment of Genotypes: To make genotyping easier and
more accurate, panels and bins text files are available for download and
are required for use with GeneMapper ® ID software. GeneMapper ® 4.0
software requires custom bin generation.
Storage Conditions: Store at –20°C. The Primer Pair Mix, Allelic Ladder and
Internal Lane Standard are light-sensitive and should be stored protected from
light.
Protocol Part#
Cell ID System Technical Manual TM074
Fluorescein:
JOE:
TMR:
A
D5S818
CXR:
Size (bp) 100 200 300 400
A = Amelogenin
TH01 D21S11
D13S317 D7S820 D16S539 CSF1PO
vWA TPOX
Allele ranges for the Cell ID System. STR fragments amplified
by the Cell ID System are labeled with different dyes and are
separated by capillary electrophoresis based on size. A size standard
is included in each sample to determine the sizes of the Cell ID-amplified
fragments. Fluorescein-labeled loci are shown in dark gray, JOE-labeled loci
are shown in gray and TMR-labeled loci are shown in white. The CXR-labeled
Internal Lane Standard 600 fragments are represented by black bars.
7426MA
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Stem Cell Research
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