2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Cell Line Authentication
292
StemElite ID System
Product Size Cat.# Price ($)
StemElite ID System 50 reactions G9530 Pls. Enq.
Available Separately Size Conc. Cat.# Price ($)
Water, Amplification Grade 6,250 µl DW0991 Pls. Enq.
9947A DNA 250 ng 10 ng/µl DD1001 194.00
Internal Lane Standard 600 150 µl DG1071 354.00
DW0991, DG1071 For Laboratory Use. G9530 For Research Use Only. Not for Use in Diagnostic
Procedures. DD1001 Not For Medical Diagnostic Use.
Description: The StemElite ID System provides the reagents required for
successful and simple identification and authentication of human cell lines and
detection of intra-species cell line cross-contamination. The StemElite ID
System uses short tandem repeat (STR) analysis of specific loci in the human
genome through co-amplification and three-color detection of ten loci (nine
STR loci and Amelogenin for gender identification), including D21S11, TH01,
TPOX, vWA, Amelogenin, CSF1PO, D16S539, D7S820, D13S317 and D5S818.
Additionally, the StemElite ID System incorporates a sensitive marker that
specifically detects the presence of DNA from mouse (Mus musculus). All
eleven loci are amplified simultaneously in a single tube. The StemElite ID
System includes a hot-start Taq DNA polymerase in an enzyme mix for convenient
room-temperature reaction assembly. Alleles, along with the allelic ladder
and an internal lane standard that assists in assigning sizes for each of the
loci, are resolved by capillary electrophoresis in a single injection, and CE data
are analyzed using allele-calling software to generate a genetic profile of the
cell line. These loci collectively provide a genetic profile with a random match
probability of 1 in 2.92 × 10 9 .
The StemElite ID System is designed specifically for use with the Applied
Biosystems 3130 and 3130xl Genetic Analyzers, ABI PRISM ® 3100 and
3100-Avant Genetic Analyzers and ABI PRISM ® 310 Genetic Analyzer.
Dye matrix generation is required on initial setup and is performed using the
PowerPlex ® Matrix Standards, 310 (Cat.# DG4640), or PowerPlex ® Matrix
Standards, 3100/3130 (Cat.# DG4650).
Features:
• Improved “Gold Standard” STR Analysis: The StemElite ID System
includes all loci used in the original PowerPlex ® 1.2 System plus the
D21S11 locus for improved power of discrimination.
• Detects Mouse Contamination: The StemElite ID System includes
a mouse locus, allowing detection of contamination of human cells with
mouse cells.
• Simplified Data Interpretation: The StemElite ID System allows
co-amplification of 10 loci to accurately discriminate between human cell
lines without using a full forensic profile and includes allelic ladders for all
10 loci to simplify data interpretation.
• Simplified Amplification Setup: Inclusion of a hot-start Taq DNA
polymerase allows room-temperature reaction assembly.
• Internal Lane Standard 600 Included: This marker offers the greatest
precision available for DNA typing. It is designed for use in each capillary
injection to increase the precision of your analyses.
• Automatic Assignment of Genotypes: To make genotyping easier and
more accurate, panels and bins text files are available for download and
are required for use with GeneMapper ® ID software. GeneMapper ® 4.0
software requires custom bin generation.
Storage Conditions: Store at –20°C. The Primer Pair Mix, Allelic Ladder and
Internal Lane Standard are light-sensitive and should be stored protected from
light.
Protocol Part#
StemElite ID System Technical Manual TM307
Fluorescein:
JOE:
TMR:
M
A
D5S818
TH01 D21S11
D13S317 D7S820 D16S539 CSF1PO
vWA TPOX
CXR:
Size (bp) 100
A = Amelogenin
M = Mouse
200 300 400
Allele ranges for the StemElite ID System. STR fragments
amplified by the StemElite ID System are labeled with different
dyes and are separated by capillary electrophoresis based on size.
A size standard is included in each sample to determine the sizes of the
StemElite ID-amplified fragments. Fluorescein-labeled loci are shown
in dark gray, JOE-labeled loci are shown in gray and TMR-labeled loci are
shown in white. The CXR-labeled Internal Lane Standard 600 fragments are
represented by black bars.
Representative data for the StemElite ID System. Genomic DNA from 9947A cells (2.0ng) combined with 20pg of mouse genomic DNA prior to
amplification was amplified using the StemElite ID System. Amplification products were mixed with Internal Lane Standard 600 and analyzed with an
Applied Biosystems 3130xl Genetic Analyzer using a 1.5kV, 5-second injection. The results were analyzed using GeneMapper ® ID software, version 3.2, and the
appropriate panels and bins text files.
For complete and up-to-date product information visit: www.promega.com/catalog
7818TA
7815MA