2012 Promega catalogue

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Life Science Catalog 2012 Worldwide Contact List Section Contents Table of Contents Cell Signaling 288 ChipShot Labeling and Clean-Up Systems Product Size Cat.# Price ($) ChipShot Indirect Labeling and Clean-Up System ChipShot Direct Labeling and Clean-Up 25 reactions Z4000 886.00 System 25 reactions Z4100 738.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The ChipShot Labeling and Clean-Up Systems enable researchers to generate consistent, reproducible and sensitive microarray data. The ChipShot Systems make it easy for individual researchers to perform RNA and microarray manipulation, regardless of their experience. The protocols are simple, time-tested and easy-to-follow. The ChipShot Direct System provides quality reagents and enzymes for generating fluorescently labeled cDNA via direct incorporation of fluorescently modified nucleotides. Labeled cDNA then can be hybridized to a printed microarray. The ChipShot Indirect System provides quality reagents and enzymes for generating fluorescently labeled cDNA using a two-step labeling process that includes synthesis of aminoallyl-modified cDNA, followed by conjugation to the CyDye NHS ester. Labeled cDNA then can be hybridized to a printed microarray. Note: Customers must supply CyDye nucleotides or NHS esters. Features: ChipShot Systems • Conserve Sample: Optimized protocols for 5μg of total RNA or 1.5μg of poly(A)+ RNA per labeling reaction. • Rely On Quality Products: Quality-controlled reagents are always available from Promega. • Simplify Your Process: Easy-to-follow protocols. • Increase Productivity: No hybridization reagent preparation and testing. • Count On Technical Support: Outstanding technical support from Promega for the researcher. ChipShot Direct System • Increase Productivity: RNA to purified, labeled cDNA in approximately 3 hours. • Get Reliable Results: Separate protocols for CyDye3 and CyDye5 labeling, so dye swap experiments are not necessary. ChipShot Indirect System • Achieve Higher Yields: Efficient incorporation of aminoallyl dNTP by reverse transcriptase yields more cDNA for labeling. • Get Unbiased Dye Incorporation: CyDye3- and CyDye5-label cDNA at a comparable rate. Storage Conditions: Store the ChipShot Direct Labeling System at –20°C, except for the RNase A, which should be stored at room temperature, and the Total RNA Positive Control, which should be stored at –70°C. Store the ChipShot Indirect Labeling System at –20°C, except for the RNase Solution, which should be stored at room temperature, and the Total RNA Positive Control, which should be stored at –70°C. Protocol Part# ChipShot Indirect Labeling and Clean-Up System Technical Manual TM287 ChipShot Direct Labeling and Clean-Up System Technical Manual TM286 cDNA Yield (ng) 2,000 1,800 1,600 1,400 1,200 1,000 800 600 400 200 0 Competitor A Competitor B ChipShot Membrane Clean-Up System Paramagnetic Particles cDNA yield achieved using the ChipShot Membrane Clean-Up System compared to competing systems. For complete and up-to-date product information visit: www.promega.com/catalog 5356MB
Cell Signaling RNA Interference GeneClip U1 Hairpin Cloning Systems Product Size Cat.# Price ($) GeneClip U1 Hairpin Cloning System—Basic 1 system C8750 553.00 GeneClip U1 Hairpin Cloning System— Puromycin GeneClip U1 Hairpin Cloning System— 1 system C8760 553.00 Hygromycin GeneClip U1 Hairpin Cloning System— 1 system C8770 553.00 Neomycin 1 system C8780 553.00 GeneClip U1 Hairpin Cloning System—hMGFP 1 system C8790 553.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The GeneClip U1 Hairpin Cloning Systems consist of linearized plasmids designed for fast and easy cloning of human target sequences to express short hairpin RNAs (shRNAs) in human cells. After transfection into human cells, in vivo expression of short interfering RNAs (siRNAs) can be effectively achieved from DNA constructs that contain a U1 RNA polymerase promoter and a siRNA template. The U1 promoter has been used successfully to generate hairpin siRNAs in vivo. To insert hairpin siRNAs into the pGeneClip Vectors, two short DNA oligonucleotides are annealed to form a DNA insert that contains the hairpin siRNA target sequence. After annealing, the oligonucleotides form overhangs that are compatible with the pGeneClip Vector ends and facilitate sticky-end ligation. Once transfected, RNA polymerase II transcribes the hairpin insert sequences to generate hairpin siRNAs in vivo. Features: • More Vector Choices: These systems provide vectors containing a variety of eukaryotic antibiotic-selectable markers for stable transfection or hMGFP for determination of transfection efficiency. • Time Savings: Vectors are supplied predigested to eliminate time- consuming vector preparation. • Convenience: Each system includes T4 DNA Ligase, 2X Rapid Ligation Buffer, Oligo Annealing Buffer and the pGeneClip Vector. • Easier Identification of Desired Clones: A PstI digestion quickly identifies positive recombinants. Storage Conditions: Store at –20°C. Protocol Part# GeneClip U1 Hairpin Cloning Systems Technical Manual TM256 For complete and up-to-date product information visit: www.promega.com/catalog T7 RiboMAX Express RNAi System Product Size Cat.# Price ($) T7 RiboMAX Express RNAi System 50 × 20µl reactions P1700 738.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The T7 RiboMAX Express RNAi System is an in vitro transcription system designed for quickly producing milligram amounts of double-stranded RNA (dsRNA). The dsRNA is free of protein and other contaminants and is suitable for use in RNA interference (RNAi) in both mammalian and nonmammalian systems. The T7 RiboMAX Express RNAi System can be used to synthesize short interfering RNAs (siRNAs) of 21bp for use in mammalian systems. siRNAs synthesized in vitro have been demonstrated to be as effective as chemically synthesized siRNAs for inducing RNAi in mammalian cells. In addition, the T7 RiboMAX Express RNAi System can be used for the synthesis of dsRNA molecules of 200bp or greater, for use in nonmammalian systems. Two complementary RNA strands are synthesized from DNA template and annealed after the transcription reaction to form dsRNA. Any remaining single-stranded RNA and DNA template are removed with a nuclease digestion step. The dsRNA is then purified by isopropanol precipitation and can be introduced into the organism of choice for RNAi applications. Features: • Save Time: The T7 RiboMAX Express RNAi System produces milligram amounts of RNA in as little as 30 minutes. • Minimize Pipetting Errors: The four rNTPs and 2X transcription buffer have been combined, thus minimizing pipetting errors and setup time. Storage Conditions: Store all components at –20°C, except RNase A, which should be stored at 22–25°C after the initial thaw. Protocol Part# T7 RiboMAX Express RNAi System Technical Bulletin TB316 % Decrease in Renilla Activity 100 80 60 40 20 0 Syn #1 IVT #1 Syn #2 IVT #2 siRNAs Site 1 Site 2 Comparison of RNA interference induced by siRNAs synthesized chemically and by in vitro transcription. Two different target luciferase sequences were synthesized by in vitro transcription using the T7 RiboMAX Express RNAi System (IVT #1 and #2) and synthesized chemically (Syn #1 and #2). After transfection using CodeBreaker Transfection Reagent, these siRNAs were evaluated for RNA interference in CHO cells stably expressing luciferase. 4218MA06_3A 289 16 RNA Analysis Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Cell Signaling Maxwell ® 16 Flexi
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Cell Signaling dNTP Mix Product Siz
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Cell Signaling 12 PCR For complete
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Cell Signaling TaqBead Hot Start Po
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Cell Signaling GoTaq ® PCR Core Sy
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Cell Signaling Deoxynucleotide Trip
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Cell Signaling Plexor ® qPCR and q
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Cell Signaling StemElite Differenti
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Cell Signaling Reverse Transcriptio
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Cell Signaling AMV Reverse Transcri
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Cell Signaling PCR Cloning pGEM ®
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Cell Signaling TnT ® Quick Coupled
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Cell Signaling TnT ® T7 Quick for
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Cell Signaling Canine Pancreatic Mi
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Cell Signaling pGEM ® β-Gal Contr
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Cell Signaling Immobilized Trypsin
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Cell Signaling Asp-N, Sequencing Gr
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Cell Signaling HaloTag ® Ligand Bu
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Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
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Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291: Cell Signaling HeLaScribe ® Nuclea
Page 295 and 296: Cell Signaling 17 Stem Cell Researc
Page 297 and 298: Cell Signaling Cell ID System Produ
Page 299 and 300: Cell Signaling StemElite Human Panc
Page 301 and 302: Cell Signaling 18 Vectors For compl
Page 303 and 304: Cell Signaling HaloTag ® Fusion (N
Page 305 and 306: Cell Signaling pCI Mammalian Expres
Page 307 and 308: Cell Signaling Reporter Vectors pGL
Page 309 and 310: Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312: Cell Signaling pGEM ® and pSP Subc
Page 313 and 314: Cell Signaling pUC/M13 Sequencing P
Page 315 and 316: Index: A-Z 19 Index For complete an
Page 317 and 318: BssHII.............................
Page 319 and 320: In vitro Transcription Systems Rela
Page 321 and 322: QuantiFluor dsDNA System ..........
Page 323 and 324: Cat# Product Size Page Price($) A10
Page 325 and 326: Index by Catalog Number Cat# Produc
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Page 333 and 334: Cat# Product Size Page Price($) G13
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Page 337 and 338: Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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