2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
288
ChipShot Labeling and Clean-Up Systems
Product Size Cat.# Price ($)
ChipShot Indirect Labeling and Clean-Up
System
ChipShot Direct Labeling and Clean-Up
25 reactions Z4000 886.00
System 25 reactions Z4100 738.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The ChipShot Labeling and Clean-Up Systems enable
researchers to generate consistent, reproducible and sensitive microarray data.
The ChipShot Systems make it easy for individual researchers to perform
RNA and microarray manipulation, regardless of their experience. The protocols
are simple, time-tested and easy-to-follow.
The ChipShot Direct System provides quality reagents and enzymes for
generating fluorescently labeled cDNA via direct incorporation of fluorescently
modified nucleotides. Labeled cDNA then can be hybridized to a printed
microarray.
The ChipShot Indirect System provides quality reagents and enzymes
for generating fluorescently labeled cDNA using a two-step labeling process
that includes synthesis of aminoallyl-modified cDNA, followed by conjugation
to the CyDye NHS ester. Labeled cDNA then can be hybridized to a printed
microarray.
Note: Customers must supply CyDye nucleotides or NHS esters.
Features:
ChipShot Systems
• Conserve Sample: Optimized protocols for 5μg of total RNA or 1.5μg of
poly(A)+ RNA per labeling reaction.
• Rely On Quality Products: Quality-controlled reagents are always
available from Promega.
• Simplify Your Process: Easy-to-follow protocols.
• Increase Productivity: No hybridization reagent preparation and testing.
• Count On Technical Support: Outstanding technical support from
Promega for the researcher.
ChipShot Direct System
• Increase Productivity: RNA to purified, labeled cDNA in approximately 3
hours.
• Get Reliable Results: Separate protocols for CyDye3 and CyDye5
labeling, so dye swap experiments are not necessary.
ChipShot Indirect System
• Achieve Higher Yields: Efficient incorporation of aminoallyl dNTP by
reverse transcriptase yields more cDNA for labeling.
• Get Unbiased Dye Incorporation: CyDye3- and CyDye5-label
cDNA at a comparable rate.
Storage Conditions: Store the ChipShot Direct Labeling System at –20°C,
except for the RNase A, which should be stored at room temperature, and
the Total RNA Positive Control, which should be stored at –70°C. Store the
ChipShot Indirect Labeling System at –20°C, except for the RNase
Solution, which should be stored at room temperature, and the Total RNA
Positive Control, which should be stored at –70°C.
Protocol Part#
ChipShot Indirect Labeling and Clean-Up System Technical
Manual
TM287
ChipShot Direct Labeling and Clean-Up System Technical Manual TM286
cDNA Yield (ng)
2,000
1,800
1,600
1,400
1,200
1,000
800
600
400
200
0
Competitor A
Competitor B
ChipShot Membrane
Clean-Up System
Paramagnetic Particles
cDNA yield achieved using the ChipShot Membrane Clean-Up
System compared to competing systems.
For complete and up-to-date product information visit: www.promega.com/catalog
5356MB