2012 Promega catalogue

Cell Signaling
HeLaScribe ® Nuclear Extract in vitro
Transcription System
Product Size Cat.# Price ($)
HeLaScribe ® Nuclear Extract in vitro
Transcription System 40 reactions E3110 692.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The most well characterized cell-free system for in vitro
transcription of eukaryotic genes is derived from HeLa cell nuclei. HeLa nuclear
extracts can support accurate transcription initiation by RNA polymerase II and
exhibit both basal and regulated patterns of RNA polymerase transcription.
The nuclear extract is also a source for a variety of transcription factors, DNAbinding
proteins and the enzymatic machinery involved in RNA processing.
The HeLa Nuclear Extract included in the HeLaScribe ® Nuclear Extract in vitro
Transcription System is prepared by a modification of the method of Dignam
et al. Extracts prepared by this method have been shown to allow transcription
from the human transferrin gene promoter and the adenovirus 2 major late
promoter. The system also includes all of the necessary components for in
vitro transcription as well as a positive control template (CMV immediate early
promoter DNA).
Features:
• Peformance-Tested: Tested with cytomegalovirus immediate early gene
(CMV) promoter.
• Convenient: Available as a complete transcription system or extract alone.
• Positive Control: System contains a CMV promoter-positive control
template.
Storage Conditions: Store at –70°C. Avoid multiple freeze-thaw cycles of the
extract.
Protocol Part#
HeLaScribe ® Nuclear Extract in vitro Transcription System Technical
Bulletin
TB123
In vitro Transcription Systems Related
Products
Product Size Cat.# Price ($)
HeLaScribe ® Nuclear Extract in vitro
40 reactions E3091 468.00
Transcription Grade
HeLaScribe
160 reactions E3092 1642.00
® Nuclear Extract Positive
Control DNA 300 ng E3621 124.00
rCTP, rATP, rUTP, rGTP, 100mM each 4 × 400 µl E6000 478.00
rATP, 100mM 400 µl E6011 138.00
rUTP, 100mM 400 µl E6021 138.00
rGTP, 100mM 400 µl E6031 138.00
rCTP, 100mM 400 µl E6041 138.00
E6000, E6011, E6021, E6031, E6041 For Laboratory Use. E3091, E3092, E3621 For Research
Use Only. Not for Use in Diagnostic Procedures.
Description: HeLaScribe ® Nuclear Extract, in vitro Transcription Grade,
derived from HeLa cell nuclei, provides a cell-free system for in vitro
transcription of eukaryotic genes.
Storage Conditions: Store HeLaScribe ® Nuclear Extracts at –70°C. Store
other components at –20°C.
Protocol Part#
HeLaScribe ® Nuclear Extract in vitro Transcription System Technical
Bulletin
TB123
For complete and up-to-date product information visit: www.promega.com/catalog
Primer Extension System—AMV Reverse
Transcriptase
Product
Primer Extension System—AMV Reverse
Size Cat.# Price ($)
Transcriptase 40 reactions E3030 266.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Primer Extension System—AMV Reverse Transcriptase can be
used to quantitate specific mRNA transcripts and map the start sites of
transcription. An end-labeled oligonucleotide is hybridized to RNA and is used
as a primer by reverse transcriptase in the presence of deoxynucleotides. The
RNA is thus reverse transcribed into cDNA and is analyzed on a denaturing
polyacrylamide gel. The length of the cDNA reflects the number of bases
between the labeled nucleotide of the primer and the 5´-end of the RNA; the
quantity of cDNA product is related to the amount of targeted RNA.
Features:
• Convenient: System includes control RNA and primer as well as size
markers ready for phosphorylation with T4 Polynucleotide Kinase.
Storage Conditions: All components must be stored at –20°C, except for the
control RNA, which must be stored at –70°C.
Protocol Part#
Primer Extension System—AMV Reverse Transcriptase Technical
Bulletin
TB113
bp
311 –
249 –
200 –
151 –
140 –
118 –
100 –
82 –
66 –
48 –
42 –
40 –
24 –
ng control RNA
M 0 10 5 2
Gel analysis of 32 P-labeled ΦX174 DNA/HinfI markers and control
RNA primer extension products produced using the Primer Extension
System— AMV Reverse Trancriptase (Cat.# E3030).
0055TA01_6A
287
16
RNA Analysis
Section
Contents
Table of
Contents