2012 Promega catalogue

More documents

Recommendations

Info

Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 24 MAO-Glo Assay Systems Product Size Cat.# Price ($) MAO-Glo Assay 200 assays V1401 223.00 1,000 assays V1402 803.00 MAO-Glo Assay with MAO-A 1,000 assays V1560 1173.00 Available Separately MAO-A For Research Use Only. Not for Use in Diagnostic Procedures. 500 µl V1452 417.00 Description: The MAO-Glo Assay provides a homogeneous luminescent method for measuring monoamine oxidase (MAO) activity from recombinant and native sources and for testing the effects of test compounds on MAO activity. The MAO-Glo Assay is performed by incubating the MAO enzyme source with a luminogenic MAO substrate. The substrate of the MAO-Glo Assay is a derivative of beetle luciferin. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to the activity of MAO. After the MAO reaction has been performed, the reconstituted Luciferin Detection Reagent is added. The reagent simultaneously stops the MAO reaction and initiates a stable glow-type luminescent signal with a half-life greater than 5 hours. This eliminates the need for strictly timed luminescent detection. The MAO-Glo Assay with MAO-A contains human recombinant MAO-A enzyme expressed in yeast. The kit is very well suited for the rapid assessment of potential inhibition of MAO-A by new chemical entities and can be used for higher throughput applications such as primary screening. The MAO-A enzyme is also available separately. The MAO-Glo Assay includes a luminogenic MAO substrate, two MAO Reaction Buffers (one that can be used with either MAO A or MAO B enzyme and one that is designed specifically for MAO B), a lyophilized Luciferin Detection Reagent and the Luciferin Detection Buffer. The user supplies the sample material containing MAO. Protocols are configured for multiwell plate formats but easily can be adapted for single-tube applications. Features: • Complete Solution: The MAO-Glo Assay with MAO-A contains monoamine oxidase A enzyme for convenient assessment of the effects of new chemical entities on MAO-A activity. • Speed: The luminescence format eliminates the need for time-consuming analyses such as HPLC. • Simplified Method: The simple “add and read” protocol makes the assay amenable to high-throughput screening in multiwell plates. • Greater Sensitivity: Less MAO enzyme is required in these assays than in typical HPLC or fluorometric methods because of the enhanced sensitivity. • No Fluorescence Interference: Luminescent output eliminates interference from fluorescent test compounds. • Stable Signal: “Glow-type” luminescence provides a stable signal with a half-life of greater than 5 hours. This eliminates the need for strictly timed luminescent detection. Storage Conditions: Store MAO-A enzyme (Cat.# V1452) at –70°C. Store all other components at –20°C protected from light. Protocol Part# MAO-Glo Assay Technical Bulletin TB345 UGT Activity Assays Product Size Cat.# Price ($) UGT-Glo Assay 200 assays V2081 480.00 1,000 assays V2082 Pls. Enq. UGT-Glo UGT1A1 Screening System 200 assays V2120 865.00 1,000 assays V2121 Pls. Enq. UGT-Glo UGT2B7 Screening System 200 assays V2130 865.00 1,000 assays V2131 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The UGT-Glo Assay is a luminescent method for measuring UDP glucuronosyltransferase (UGT) activity. The UGT-Glo Assay measures UGT activity from a variety of sources, such as microsomes containing recombinantly expressed enzymes or microsomal preparations derived from mammalian tissues, and to test the effects of various chemicals on UGT activity. The assay involves incubating UGT with a proluciferin substrate; a portion of the substrate gets conjugated with UDP, while the remainder is unmodified. Upon the addition of d-Cysteine, the unconjugated proluciferin is converted into luciferin and, in a coupled reaction with luciferase/luciferin, is converted into light. Conjugated proluciferin remains intact and does not contribute to the luminescence. Thus, the signal generated is inversely correlated with UGT activity present in the sample. The UGT-Glo Assay contains two proluciferin substrates: the UGT Multienzyme Substrate, which is compatible with a wide range of UGTs, and the UGT1A4 Substrate, which reacts specifically with UGT1A4. The kit also contains Luciferin Detection Reagent and Reconstitution Buffer, UGT Buffer, d-Cysteine and UDPGA. The UGT-Glo Screening Systems contain the above reagents as well as the respective UGT isoforms and control membranes. Features: • Speed: The luminescent format eliminates the need for time-consuming analyses such as HPLC and LC/MS. • Simplified Method: The simple “add and read” protocol makes the assay amenable to higher throughput screening in multiwell plates. • Sensitive: Allows researchers to use less enzyme and scale down reaction volumes, which saves on reagent costs. Storage Conditions: Store UGT enzymes and Control Membranes at –70°C. Store remaining components at –20°C. Protocol Part# UGT-Glo Assay Technical Bulletin TB551 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling Apoptosis VIABLE T 0 NECROSIS For complete and up-to-date product information visit: www.promega.com/catalog REDUCED VIABILITY T1 T 2 APOPTOSIS Parameter/Biomarker Time to Sensitivity Assay Type Measured Results (*384 well) Plate Format Instrument CellTiter-Glo ® Assay Viable cell ATP 10 minutes 10 viable cells* 96/384/1536 Luminometer/CCD CellTiter-Fluor Assay Live-cell protease 0.5–3 hours 40 viable cells 96/384/1536 Fluorometer AFC 400nmEx/505nmEm CellTiter-Blue ® Assay Resazurin reduction by NADH 1–4 hours 50 cells* 96/384/1536 Fluorometer, Resorufi n 560nmEx/590nmEm CellTiter 96 ® AQueous One Solution Assay MTS reduction by NADH 1–4 hours 200 cells* 96/384 Spectrophotometer Abs 490nm MultiTox-Glo Assay MultiTox-Fluor Assay Viability and cytotoxicity by live- and dead-cell proteases Viability and cytotoxicity by live- and dead-cell proteases 0.5 hour 0.5–3 hours 40 viable cells, 10 dead cells 40 live cells, 10 dead cells 96/384/1536 96/384/1536 Fluorometer AFC 400nm Ex/505nm Em Luminometer Fluorometer AFC 400nm Ex/505nm Em R110 485nm Ex/520nm Em CytoTox-Glo Assay Dead-cell protease release 15 minutes 10 dead cells 96/384/1536 Luminometer CytoTox-Fluor Assay Dead-cell protease release 0.5–3 hours 10 dead cells 96/384 Fluorometer R110 485nmEx/520nmEm CytoTox-ONE Assay LDH release 10 minutes 200 cells* 96/384 Fluorometer, Resorufi n 560nmEx/590nmEm Caspase-Glo ® 3/7 Assay Caspase-3/7 activity 0.5 hour 20 cells* 96/384/1536 Luminometer Apo-ONE ® Caspase 3/7 Assay Caspase-3/7 activity 1–18 hours 200 cells* 96/384/1536 Fluorometer R110 499nmEx/521nmEm Caspase-Glo ® 8 Assay Caspase-8 activity 0.5 hour ~1000 cells 96 Luminometer Caspase-Glo ® 9 Assay Caspase-9 activity 0.5 hour ~1500 cells 96 Luminometer Mitochondrial ToxGlo Assay ATP Dead cell protease 1 hour 10 viable cells 10 dead cells 96/394 Luminometer GSH-Glo Assay GSH 30 minutes 96\384 Luminometer GSH/GSSG-Glo Assay GSH/GSSG 1hour 96\384 Luminometer 9481LA 25 2 Cell Health Assays Section Contents Table of Contents
Page 1 and 2: Life Science CATALOG 2012
Page 3 and 4: Cell Signaling Table of Contents Pr
Page 5 and 6: Cell Signaling For complete and up-
Page 7 and 8: Cell Signaling Agarose, LMP, Prepar
Page 9 and 10: Cell Signaling Blue/Orange Loading
Page 11 and 12: Cell Signaling Glycine, Molecular B
Page 13 and 14: Cell Signaling Sephacryl ® S-400 P
Page 15 and 16: Cell Signaling Tris Base, Molecular
Page 17 and 18: Cell Signaling Unmethylated Lambda
Page 19 and 20: Cell Signaling Promega Barrier Tips
Page 21 and 22: Cell Signaling Product Size Cat.# P
Page 23 and 24: Cell Signaling 2 Cell Health Assays
Page 25 and 26: Cell Signaling A. B. Luminescence/p
Page 27: Cell Signaling Luminogenic Enzyme S
Page 31 and 32: Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34: Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36: Cell Signaling CaspACE Assay System
Page 37 and 38: Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40: Cell Signaling BacTiter-Glo Microbi
Page 41 and 42: Cell Signaling CellTiter 96 ® Cell
Page 43 and 44: Cell Signaling MultiTox-Fluor Multi
Page 45 and 46: Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48: Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50: Cell Signaling GSH-Glo Glutathione
Page 51 and 52: Cell Signaling 3 Cell Signaling For
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76: Cell Signaling For complete and up-
Page 77 and 78: Cell Signaling DNA Step Ladders Pro
Page 79 and 80:
Cell Signaling Conventional DNA Mar
Page 81 and 82:
Cell Signaling ProMega-Markers ® L
Page 83 and 84:
Cell Signaling Restriction Enzymes
Page 85 and 86:
Cell Signaling BamHI Product Size C
Page 87 and 88:
Cell Signaling EcoRI Product Size C
Page 89 and 90:
Cell Signaling NciI Product Size Co
Page 91 and 92:
Cell Signaling SpeI Product Size Co
Page 93 and 94:
Cell Signaling TSAP Thermosensitive
Page 95 and 96:
Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98:
Cell Signaling Kinases T4 Polynucle
Page 99 and 100:
Cell Signaling S1 Nuclease Product
Page 101 and 102:
Cell Signaling Percent Inhibition o
Page 103 and 104:
Cell Signaling Untagged Flexi ® Ma
Page 105 and 106:
Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108:
Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110:
Cell Signaling For complete and up-
Page 111 and 112:
Cell Signaling x-tracta Gel Extract
Page 113 and 114:
Cell Signaling Wizard ® MagneSil
Page 115 and 116:
Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118:
Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120:
Cell Signaling MagneSil ® KF, Geno
Page 121 and 122:
Cell Signaling Plasmid Purification
Page 123 and 124:
Cell Signaling PureYield Plasmid Ma
Page 125 and 126:
Cell Signaling Wizard ® Plus Maxip
Page 127 and 128:
Cell Signaling Wizard ® MagneSil
Page 129 and 130:
Cell Signaling PureYield RNA Midipr
Page 131 and 132:
Cell Signaling Maxwell ® 16 System
Page 133 and 134:
Cell Signaling RNasin ® Plus RNase
Page 135 and 136:
Cell Signaling DNA Quantitation Qua
Page 137 and 138:
Cell Signaling Magnetic Stands and
Page 139 and 140:
Cell Signaling 6 Drug Discovery For
Page 141 and 142:
Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144:
Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146:
Cell Signaling Protease Assays Prot
Page 147 and 148:
Cell Signaling Cell-Based Proteasom
Page 149 and 150:
Cell Signaling Tryptase, Human, Rec
Page 151 and 152:
Cell Signaling 7 Genetic Identity F
Page 153 and 154:
Cell Signaling Maxwell ® 16 Forens
Page 155 and 156:
Cell Signaling Plexor ® HY System
Page 157 and 158:
Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160:
Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162:
Cell Signaling PowerPlex ® 16 and
Page 163 and 164:
Cell Signaling GenePrint ® Fluores
Page 165 and 166:
Page 167 and 168:
Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170:
Cell Signaling Block & Sample 5X Bu
Page 171 and 172:
Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174:
Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176:
Cell Signaling Anti-Human GDNF pAb
Page 177 and 178:
Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180:
Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182:
Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184:
Page 185 and 186:
Cell Signaling Microbial Detection
Page 187 and 188:
10 Instruments For complete and up-
Page 189 and 190:
GloMax ® 20/20 Luminometer Product
Page 191 and 192:
Fluorescence Module: Application-op
Page 193 and 194:
GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196:
Maxwell ® 16 Instruments Maxwell
Page 197 and 198:
Maxwell ® 16 Flexi Method Firmware
Page 199 and 200:
Page 201 and 202:
Cell Signaling Maxwell ® 16 System
Page 203 and 204:
Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206:
Cell Signaling dNTP Mix Product Siz
Page 207 and 208:
Cell Signaling 12 PCR For complete
Page 209 and 210:
Cell Signaling TaqBead Hot Start Po
Page 211 and 212:
Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214:
Cell Signaling Deoxynucleotide Trip
Page 215 and 216:
Cell Signaling Plexor ® qPCR and q
Page 217 and 218:
Cell Signaling StemElite Differenti
Page 219 and 220:
Cell Signaling Reverse Transcriptio
Page 221 and 222:
Cell Signaling AMV Reverse Transcri
Page 223 and 224:
Cell Signaling PCR Cloning pGEM ®
Page 225 and 226:
Page 227 and 228:
Cell Signaling TnT ® Quick Coupled
Page 229 and 230:
Cell Signaling TnT ® T7 Quick for
Page 231 and 232:
Cell Signaling Canine Pancreatic Mi
Page 233 and 234:
Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236:
Cell Signaling Immobilized Trypsin
Page 237 and 238:
Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240:
Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242:
Cell Signaling Inducible T7-Driven
Page 243 and 244:
Cell Signaling ProtoBlot ® II AP S
Page 245 and 246:
Page 247 and 248:
Cell Signaling HaloTag ® Protein P
Page 249 and 250:
Cell Signaling HaloTag ® Mammalian
Page 251 and 252:
Cell Signaling HaloLink Protein Arr
Page 253 and 254:
Cell Signaling HaloCHIP System Prod
Page 255 and 256:
Cell Signaling MagneHis Protein Pur
Page 257 and 258:
Cell Signaling Streptavidin Product
Page 259 and 260:
Cell Signaling Protein Purification
Page 261 and 262:
Cell Signaling 15 Reporter Assays a
Page 263 and 264:
Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266:
Cell Signaling Firefly Luciferase R
Page 267 and 268:
Cell Signaling Bright-Glo Luciferas
Page 269 and 270:
Cell Signaling EnduRen Live Cell Su
Page 271 and 272:
Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274:
Cell Signaling Promoter-Driven Cont
Page 275 and 276:
Cell Signaling Nuclear Receptor Pat
Page 277 and 278:
Cell Signaling pRL Renilla Lucifera
Page 279 and 280:
Cell Signaling GloResponse Lucifera
Page 281 and 282:
Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284:
Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286:
Cell Signaling Transfection Reagent
Page 287 and 288:
Cell Signaling 16 RNA Analysis For
Page 289 and 290:
Cell Signaling Riboprobe ® System
Page 291 and 292:
Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294:
Cell Signaling RNA Interference Gen
Page 295 and 296:
Cell Signaling 17 Stem Cell Researc
Page 297 and 298:
Cell Signaling Cell ID System Produ
Page 299 and 300:
Cell Signaling StemElite Human Panc
Page 301 and 302:
Cell Signaling 18 Vectors For compl
Page 303 and 304:
Cell Signaling HaloTag ® Fusion (N
Page 305 and 306:
Cell Signaling pCI Mammalian Expres
Page 307 and 308:
Cell Signaling Reporter Vectors pGL
Page 309 and 310:
Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312:
Cell Signaling pGEM ® and pSP Subc
Page 313 and 314:
Cell Signaling pUC/M13 Sequencing P
Page 315 and 316:
Index: A-Z 19 Index For complete an
Page 317 and 318:
BssHII.............................
Page 319 and 320:
In vitro Transcription Systems Rela
Page 321 and 322:
QuantiFluor dsDNA System ..........
Page 323 and 324:
Cat# Product Size Page Price($) A10
Page 325 and 326:
Index by Catalog Number Cat# Produc
Page 327 and 328:
Cat# Product Size Page Price($) C84
Page 329 and 330:
Cat# Product Size Page Price($) E13
Page 331 and 332:
Cat# Product Size Page Price($) E61
Page 333 and 334:
Cat# Product Size Page Price($) G13
Page 335 and 336:
Cat# Product Size Page Price($) G77
Page 337 and 338:
Cat# Product Size Page Price($) L50
Page 339 and 340:
Cat# Product Size Page Price($) P16
Page 341 and 342:
Cat# Product Size Page Price($) R99
Page 343 and 344:
Cat# Product Size Page Price($) V42
Page 345 and 346:
Cat# Product Size Page Price($) W39
Page 347 and 348:
personal DETECTION GloMax Integrat
show all

2012 Promega catalogue

Create successful ePaper yourself

Delete template?

Save as template?