2012 Promega catalogue

Cell Signaling
Riboprobe ® System Components and Buffers
Product Size Conc. Cat.# Price ($)
Riboprobe ® System Buffers 1 system P1121 146.00
rATP, rCTP, rGTP, rUTP, each at 10mM
in separate tubes
Available Separately
0.5 ml 10 mM P1221 187.00
RQ1 RNase-Free DNase 1,000 u 1 u/µl M6101 86.00
rATP, 10mM 0.5 ml 10 mM P1132 69.00
rCTP, 10mM 0.5 ml 10 mM P1142 69.00
rGTP, 10mM 0.5 ml 10 mM P1152 69.00
rUTP, 10mM 0.5 ml 10 mM P1162 69.00
DTT, Molecular Grade 100 µl 100 mM P1171 47.00
Transcription Optimized 5X Buffer 200 µl P1181 47.00
Nuclease-Free Water
For Laboratory Use.
50 ml P1193 70.00
Description: Riboprobe ® System Buffers are components of the single and
combination Riboprobe ® Systems. The buffers are also available as standalone
products.
RQ1 RNase-Free DNase is used to remove template DNA from RNA
preparations and is qualified for use in applications where maintaining the
integrity of RNA is critical. Product is quality tested to ensure the absence of
detectable RNase activity. 10X Reaction Buffer and 10X Stop Buffer included.
rATP, rCTP, rGTP and rUTP are provided in individual tubes, qualified for use
with the Riboprobe ® Systems. The rNTPs are supplied in nuclease-free water.
Purity has been verified by HPLC analysis.
Features:
• Pretested: Reagents are tested with other Riboprobe ® System components.
rNTPs are tested for functionality with in vitro transcription reactions.
• Transcription Qualified: Reagents are qualified for use for in vitro
transcription reactions with SP6, T7 or T3 RNA Polymerase.
Storage Conditions: Store at –20°C.
Protocol Part#
Riboprobe ® In Vitro Transcription Systems Technical Manual TM016
Ribo m 7 G Cap Analog
Product Size Conc. Cat.# Price ($)
Ribo m7G Cap Analog 10 A254 units 40 mM P1711 354.00
25 A254 units 40 mM P1712 673.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Ribo m 7 G Cap Analog is a modified ribonucleotide with the
structure (m 7 G(5´)ppp(5´)G). This methylated ribonucleotide can be incorporated
onto the 5´-end of transcripts synthesized in vitro and simulates the 7-methyl
guanosine 5´-cap structure found on most eukaryotic mRNA molecules.
Features:
• Improved Translation: Enhances translation efficiency in many
reticulocyte-based reactions.
• Effective: Protects RNA from intracellular digestion.
• Flexible: Can be used in either the Riboprobe ® Systems or RiboMAX
Large Scale RNA Production Systems.
Storage Conditions: Store at –20°C.
Protocol Part#
Ribo m7G Cap Analog Protocol 9PIP171
For complete and up-to-date product information visit: www.promega.com/catalog
pGEM ® Express Positive Control Template
Product Size Cat.# Price ($)
pGEM ® Express Positive Control Template
For Research Use Only. Not for Use in Diagnostic Procedures.
10 µg P2561 85.00
Description: The pGEM ® Express Positive Control Template is created by
linearizing a vector with the restriction enzyme ScaI. The Positive Control
Template may be used to monitor in vitro transcription reactions when using
the Riboprobe ® Systems.
Features:
• Multi-Sized RNAs: SP6 RNA polymerase produces transcripts of 1,787
and 2,566 bases; T7 RNA polymerase produces transcripts of 1,065 and
2,346 bases; T3 RNA Polymerase produces transcripts of 250 and 1,525
bases.
• Flexible: Template can be used with SP6, T7 or T3 RNA polymerases.
Storage Conditions: Store at –20°C.
Protocol Part#
pGEM ® Express Positive Control Template Product Information 9PIP256
AP1, (c-Jun), Human, Recombinant
Product Size Cat.# Price ($)
rhAP1 (c-Jun)
For Research Use Only. Not for Use in Diagnostic Procedures.
50 fpu E3061 402.00
Description: c-Jun is the protein encoded by the human proto-oncogene, cjun.
Its expression is greatly modulated by extracellular stimuli such as phorbol
ester and growth factor treatment. It is a member of the AP1 family. In many
cell types, AP1 activity is composed primarily of Jun and Fos heterodimers and
secondarily of Jun homodimers as in HeLa cells. Jun homodimers bind AP1
sites in vitro and activate transcription. c-Jun is expressed in E. coli from a
human cDNA clone and has a molecular weight of 40kDa.
Features:
• Performance-Tested: Tested in gel-shift and footprinting applications.
Storage Conditions: Store at –70°C.
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16
RNA Analysis
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