2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
In vitro Transcription and Microarrays
284
RiboMAX Large Scale RNA Production
Systems
Product Size Cat.# Price ($)
RiboMAX Large Scale RNA Production
System—SP6
RiboMAX Large Scale RNA Production
1 system P1280 639.00
System—T7
For Laboratory Use.
1 system P1300 639.00
Description: The RiboMAX Large Scale RNA Production Systems produce
2–5mg/ml of RNA in a 1ml reaction, about 10- to 20-fold more RNA than is
produced with the standard Riboprobe ® System transcription reaction. The
RiboMAX System reactions differ from those of the Riboprobe ® Systems in
three primary ways: a HEPES (pH 7.5) buffer is used rather than a Tris-HCl
(pH 7.9) buffer; rNTP and magnesium concentrations are elevated at levels
appropriate for either SP6 or T7 RNA polymerase; and inorganic
pyrophosphatase is included in the reaction.
RNAs synthesized with the RiboMAX System perform better for in vitro
translation in rabbit reticulocyte translation systems than RNA synthesized by
standard methods. The reduction of components inhibitory to translation may be
advantageous for other applications requiring biologically active RNA. Because
the RiboMAX Systems produce large quantities of RNA, these systems are not
recommended for the generation of high-specific-activity RNA probes.
Features:
• Flexible: Systems are available for use with SP6 and T7 RNA polymerases.
• Scalable: Reactions can be scaled up or down to suit varying RNA
production requirements.
• High-Quality: Synthesis of enhanced, translation-grade RNA.
Storage Conditions: Store at –20°C.
Protocol Part#
RiboMAX Large Scale RNA Production Systems—SP6 and T7
Technical Bulletin
TB166
T7 RiboMAX Express Large Scale RNA
Production System
Product
T7 RiboMAX Express Large Scale RNA
Size Cat.# Price ($)
Production System
For Laboratory Use.
1 system P1320 727.00
Description: The T7 RiboMAX Express Large Scale RNA Production System
is an in vitro transcription system designed for the consistent production of
milligram amounts of RNA in a short amount of time. Due to optimization of the
enzyme mix and transcription buffer, yields of 5–8.5mg/ml are generated in 30
minutes, compared to 2–4 hours with other commercially available systems. To
minimize pipetting steps and errors, the 2X transcription buffer includes all four
rNTPs. In addition, the system includes RQ1 RNase-Free DNase for the removal
of plasmid template after transcription.
Due to the combined 2X buffer and rNTPs, the T7 RiboMAX Express
System is not recommended for the synthesis of RNA for applications that
require capped RNA. For synthesis of capped RNA, please order the standard
RiboMAX Large Scale RNA Production System—T7 (Cat.# P1300).
Features:
• Fast: The T7 RiboMAX Express System produces milligram amounts of
RNA in as little as 30 minutes rather than 2–4 hours as with other
commercially available systems.
• Convenient: The four rNTPs and 2X transcription buffer have been
combined, thus minimizing pipetting errors and setup time.
• Flexible: Efficiently transcribes DNA templates of varying sizes. Works with
transcripts as short as 21bp.
Storage Conditions: Store at –20°C.
Protocol Part#
T7 RiboMAX Express Large Scale RNA Production System TB298
Riboprobe ® Systems
Product Size Cat.# Price ($)
Riboprobe ® System—SP6 1 system P1420 423.00
Riboprobe ® System—T3 1 system P1430 372.00
Riboprobe ® System—T7 1 system P1440 383.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Riboprobe ® Systems are designed for in vitro preparation
of high-specific-activity single-stranded RNA probes or microgram quantities
of defined RNA transcripts from cloned DNA inserts. These systems contain all
components necessary for in vitro transcription from a DNA template (excluding
the radioisotope) and also contain RQ1 RNase-Free DNase (Cat.# M6101) for
template removal following transcription.
Features:
• Specific: SP6, T7 and T3 RNA Polymerases are extremely promoterspecific,
allowing production of virtually homogeneous RNA using plasmid
DNA as a template.
• Choice of Enzyme: Systems available with SP6 RNA Polymerase, T7 RNA
Polymerase or T3 RNA Polymerase.
• Convenient: Includes positive control template for use with SP6, T7 or T3
RNA Polymerase, DNase I for removal of DNA template and Recombinant
RNasin ® Ribonuclease Inhibitor.
Storage Conditions: Store at –20°C.
Protocol Part#
Riboprobe ® In Vitro Transcription Systems Technical Manual TM016
Riboprobe ® Combination Systems
Product
Riboprobe
Size Cat.# Price ($)
® Combination System—T3/T7 RNA
Polymerase
Riboprobe
1 system P1450 450.00
® Combination System—SP6/T7 RNA
Polymerase 1 system P1460 493.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Riboprobe ® Combination Systems are designed for in vitro
preparation of high-specific-activity single-stranded RNA probes or microgram
quantities of defined RNA transcripts from cloned DNA inserts. The Riboprobe ®
Combination Systems include the RNA polymerases, all of the required
reagents (excluding radioisotope) for performing transcription reactions in vitro
and RQ1 RNase-Free DNase (Cat.# M6101) for removal of the template
following transcription.
Features:
• Flexible: Allows synthesis of RNA corresponding to either the coding or
noncoding strand of cloned DNA from a single plasmid construct.
• Specific: SP6, T7 and T3 RNA Polymerases are extremely promoterspecific,
allowing production of virtually homogeneous RNA using plasmid
DNA as a template.
• Convenient: Includes positive control template for use with T7, T3 or SP6
RNA polymerase, DNase I for removal of DNA template and Recombinant
RNasin ® Ribonuclease Inhibitor.
Storage Conditions: Store at –20°C.
Protocol Part#
Riboprobe ® In Vitro Transcription Systems Technical Manual TM016
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