2012 Promega catalogue

Life
Science
Catalog
2012
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Cell Signaling
282
TransFast Transfection Reagent
Product Size Cat.# Price ($)
TransFast Transfection Reagent 1.2 mg E2431 519.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The TransFast Transfection Reagent is composed of the
synthetic cationic lipid, (+)-N,N [bis (2-hydroxyethyl)]-N-methyl-N-[2,3di(tetradecanoyloxy)propyl]
ammonium iodide and the neutral lipid, DOPE. The
TransFast Reagent is supplied as a dried lipid film that forms multilamellar
vesicles upon hydration with water. Cationic liposomes designed for
transfection, such as the TransFast Reagent, are more versatile than many
other traditional transfection methods. The advantages include flexibility in the
macromolecules that are delivered, in vitro and in vivo applications, ability to
more reproducibly transfect cells that are recalcitrant to other methods and
suitability for transient and stable transfection. Several different types of
macromolecules, including RNA and DNA of all sizes ranging from
oligonucleotides to plasmids and yeast artificial chromosomes, can be delivered
to cells using liposomes. The TransFast Transfection Reagent is designed
for nucleic acid delivery to eukaryotic cells in vitro and in vivo and performs
well with many cell lines. To date, we have found that TransFast Reagent
performs particularly well for DNA delivery to NIH/3T3, CHO, 293, K562, PC12,
Jurkat and insect Sf9 cells.
Features:
• Fast: Transfect in 1 hour. Transfection times can be decreased to as little
as 30 minutes with certain cell lines.
• Easy to Use: Resuspend the reagent in water, freeze, thaw, mix with DNA,
and add to cells.
• Efficient: High-efficiency transfection—transient and stable—in many
cells.
• Robust: Requires less optimization than other systems. Allows transfection
of cell types such as primary cell cultures that require continuous exposure
to serum.
Storage Conditions: Store at –20°C.
Protocol Part#
TransFast Transfection Reagent Technical Bulletin TB260
ProFection ® Mammalian Transfection System
Product Size Cat.# Price ($)
ProFection ® Mammalian Transfection
System—Calcium Phosphate 40 reactions E1200 238.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The introduction of DNA into mammalian cells is facilitated by
the ProFection ® Mammalian Transfection System. This system offers you a
Calcium Phosphate-mediated transfection procedure. Each system contains
sufficient reagents for 40 high-efficiency transfections of cells plated in
100mm tissue culture dishes.
Calcium phosphate transfection is an effective method for the production
of long-term stable transfectants. This method also works well for transient
expression of transfected genes and can be used with most adherent cell lines.
Features:
• Efficient: Components optimized for high transfection efficiencies.
Storage Conditions: Store at –20°C.
Protocol Part#
ProFection ® Mammalian Transfection System Technical Manual TM012
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