2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
280<br />
ViviRen In Vivo Renilla Luciferase<br />
Substrate<br />
Product Size Cat.# Price ($)<br />
ViviRen In Vivo Renilla Luciferase Substrate 0.37 mg P1231 192.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
3.7 mg P1232 Pls. Enq.<br />
Description: ViviRen in vivo Renilla Luciferase Substrate is a uniquely<br />
engineered coelenterazine-based compound with protected oxidation sites.<br />
These modifications are designed to minimize substrate degradation and autoluminescence.<br />
It is reported that the ViviRen Substrate demonstrates brighter<br />
output when compared to the native coelenterazine substrate when used in an<br />
in vivo imaging application in a mouse model.<br />
Cat.# P1231 is supplied as a liquid, 60mM in DMSO. Cat.# P1232 is supplied<br />
as a lyophilized solid.<br />
VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen<br />
Corporation and Caliper Life Sciences for use in in vivo bioluminescence<br />
imaging applications.<br />
Features:<br />
• Highest Quality Substrates: Eliminate potential interference in assays<br />
due to the presence of endotoxins.<br />
• Assured Product Integrity: Most products are packaged in amber vials<br />
with septa to ensure product integrity as well as offer ease of dilution and<br />
use for imaging experiments. Product is packaged with fine tolerances to<br />
minimize the need to weigh substrates.<br />
• Flexibility and Convenience: Available in multiple sizes to accommodate<br />
a variety of experimental settings.<br />
Storage Conditions: Store at –20°C.<br />
9540MA<br />
pGL4 in vivo Imaging Vectors<br />
Product Size Cat.# Price ($)<br />
pGL4.50[luc2/CMV/Hygro] Vector 20 µg E1310 545.00<br />
pGL4.51[luc2/CMV/Neo] Vector<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
20 µg E1320 545.00<br />
Description: The pGL4 Luciferase Reporter Vectors are the next generation of<br />
reporter gene vectors optimized for expression in mammalian cells. Numerous<br />
configurations of pGL4 Vectors are available, including those with the synthetic<br />
firefly luc2 (Photinus pyralis) and Renilla hRluc (Renilla reniformis) genes, which<br />
have been codon optimized for more efficient expression in mammalian cells.<br />
Furthermore, both the reporter genes and the vector backbone, including the<br />
ampicillin (Ampr ) gene and mammalian selectable marker genes for hygromycin<br />
(Hygr ), neomycin (Neor ) and puromycin (Puror ), have been engineered to<br />
reduce the number of consensus transcription factor binding sites, reducing<br />
background and the risk of anomalous transcription.<br />
The pGL4 Vector backbone is provided with either the luc2 or hRluc gene and,<br />
in certain vectors, one or both of two Rapid Response Reporter genes. The<br />
proteins encoded by these Rapid Response Luciferase genes respond more<br />
quickly and with greater magnitude to changes in transcriptional activity than<br />
their more stable counterparts. Vectors are available with a minimal promoter<br />
upstream of the lucP reporter gene, useful for cloning in a response element of<br />
choice. Vectors also are available with a minimal promoter plus choice of<br />
response elements or other promoter elements that can be used to study<br />
cellular signaling and other events. These vectors can be used in transient<br />
transfections or to generate a stable cell line. All vectors use a destabilized<br />
luc2P firefly luciferase gene, resulting in low backgrounds and high levels of<br />
induction. Stable cell selection is possible using hygromycin resistance.<br />
Improved Sensitivity and Biological Relevance Due to:<br />
• Increased Reporter Gene Expression: Codon optimization of synthetic<br />
genes for mammalian expression.<br />
• Reduced Background and Risk of Expression Artifacts: Removal of<br />
cryptic DNA regulatory elements and transcription factor binding sites.<br />
• Improved Temporal Response: Rapid Response technology available<br />
using destabilized luciferase genes.<br />
Additional Advantages Include:<br />
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus<br />
pyralis) or hRluc (Renilla reniformis) reporter genes.<br />
• Easy Transition from Transient to Stable Cells: Choice of mammalian<br />
selectable markers.<br />
• Easy Transfer from Vector to Vector: Common multiple cloning site and<br />
a unique SfiI transfer scheme.<br />
Storage Conditions: Store at –20°C.<br />
For complete and up-to-date product information visit: www.promega.com/catalog