2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
278
pCAT ® 3 Vectors
Product Size Cat.# Price ($)
pCAT ® 3-Basic Vector 20 µg E1871 214.00
pCAT ® 3-Control Vector 20 µg E1851 214.00
pCAT ® 3-Enhancer Vector 20 µg E1881 214.00
pCAT ® 3-Promoter Vector 20 µg E1861 214.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The pCAT ® 3 Reporter Vectors provide a basis for the
quantitative analysis of factors that may regulate mammalian gene expression.
The redesigned backbone of the pCAT ® 3 Reporter Vectors is similar to the
pGL3 Luciferase Vectors with the exception of a chimeric intron located 5´ of
the chloramphenicol acetyltransferase (CAT) gene. As with the pGL3 Vectors,
the pCAT ® 3 Vectors contain a different polyadenylation site located 3´ of the
gene. The redesigned backbone increases expression of the reporter gene,
improves in vivo vector stability and provides greater flexibility in performing
manipulations.
Features:
• Efficient: Optimal translation efficiency.
• Robust: Increased expression with more efficient poly(A) signal.
• Clearer Results: Reduced background CAT expression.
• Compatible: Altered multiple cloning regions make vectors compatible
with the pGL3 Vectors.
• Versatile: Can produce ssDNA for sequencing and mutagenesis.
Storage Conditions: Store vectors at –20°C.
Protocol Part#
pCAT ® 3 Reporter Vectors Technical Manual
TM036
Monster Green ® Fluorescent Protein phMGFP
Vector
Product Size Cat.# Price ($)
Monster Green ® Fluorescent Protein phMGFP Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg E6421 836.00
Description: The phMGFP Vector contains the open reading frame for the
Monster Green ® Fluorescent Protein cloned into a mammalian expression
vector. The Monster Green ® Fluorescent Protein is encoded by an improved
synthetic version of the green fluorescent protein gene originally cloned from
Montastrea cavernosa (Great Star Coral). The synthetic gene (hMGFP)
expresses a 26kDa protein that shows improved fluorescence intensity
compared to the native gene. Furthermore, the hMGFP gene has been codon
optimized and cleared of most consensus sequence transcription factor binding
sites to ensure reliability and high levels of expression.
The Monster Green ® Fluorescent Protein encoded by the hMGFP gene is an
ideal fluorescent reporter, providing high-level fluorescence and reducing
cytotoxicity. Monster Green ® Fluorescent Protein generally fluoresces at least
20% brighter than other commercially available green fluorescent proteins
(GFPs) and also reduces cytotoxicity, offering flexibility when working with
transient and stable expression assays.
Features:
• Brighter Fluorescence: Visualize low-level expression in situ using
fluorescence microscopy, imagers or FACS ® .
• Reduced Cytotoxicity: Minimize cellular perturbations when working with
transient or stable expression assays.
• Flexible: Create fusion proteins for imaging and localization studies using
standard FITC detection.
• High Purity: Obtain high transfection efficiencies for precloning confirmation
studies.
Storage Conditions: Store at –20°C.
Protocol Part#
Monster Green ® Fluorescent Protein phMGFP Vector Technical
Bulletin
TB320
Amp r
ori
phMGFP
Vector
(4707bp)
SV40 Late
poly(A)
NotI
Intron
Bst98I
PstI
Bst98I
NheI
XmaI/SmaI
EcoRV
NcoI
For complete and up-to-date product information visit: www.promega.com/catalog
BglII
NaeI
XbaI
CMV I. E.
Enhancer/Promoter
NcoI
T7
hMGFP
Bioluminescence Imaging Technology
VivoGlo Luciferin, In Vivo Grade
Product Size Cat.# Price ($)
VivoGlo Luciferin, In Vivo Grade 50 mg P1041 269.00
250 mg P1042 569.00
For Research Use Only. Not for Use in Diagnostic Procedures.
1 g P1043 1199.00
Description: Luciferase genes from the North American firefly (Photinus
pyralis) and from other beetles are commonly used as light-emitting reporters
in cellular and animal models. VivoGlo Luciferin is the potassium salt of
d-luciferin, the firefly luciferase substrate capable of generating light when a
suitable model is used.
VivoGlo In Vivo Imaging Substrates are provided in cooperation with Xenogen
Corporation and Caliper Life Sciences for use in in vivo bioluminescence
imaging applications.
Features:
• Highest Quality Substrates: Eliminate potential interference in assays
due to the presence of endotoxins.
• Assured Product Integrity: Most products are packaged in amber vials
with septa to ensure product integrity as well as offer ease of dilution and
use for imaging experiments. Product is packaged with fine tolerances to
minimize the need to weigh substrates.
• Flexibility and Convenience: Available in multiple sizes to accommodate
a variety of experimental settings.
Storage Conditions: Store at –20°C.
3898MA11_2A
9540MA