2012 Promega catalogue

Life
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2012
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Cell Signaling
24
MAO-Glo Assay Systems
Product Size Cat.# Price ($)
MAO-Glo Assay 200 assays V1401 223.00
1,000 assays V1402 803.00
MAO-Glo Assay with MAO-A 1,000 assays V1560 1173.00
Available Separately
MAO-A
For Research Use Only. Not for Use in Diagnostic Procedures.
500 µl V1452 417.00
Description: The MAO-Glo Assay provides a homogeneous luminescent
method for measuring monoamine oxidase (MAO) activity from recombinant
and native sources and for testing the effects of test compounds on MAO
activity. The MAO-Glo Assay is performed by incubating the MAO enzyme
source with a luminogenic MAO substrate. The substrate of the MAO-Glo
Assay is a derivative of beetle luciferin. Upon reaction with MAO, the derivative
is converted into luciferin, which in turn reacts with luciferase to produce light.
The amount of light produced is directly proportional to the activity of MAO.
After the MAO reaction has been performed, the reconstituted Luciferin Detection
Reagent is added. The reagent simultaneously stops the MAO reaction and
initiates a stable glow-type luminescent signal with a half-life greater than 5
hours. This eliminates the need for strictly timed luminescent detection.
The MAO-Glo Assay with MAO-A contains human recombinant MAO-A
enzyme expressed in yeast. The kit is very well suited for the rapid assessment
of potential inhibition of MAO-A by new chemical entities and can be used for
higher throughput applications such as primary screening. The MAO-A enzyme
is also available separately.
The MAO-Glo Assay includes a luminogenic MAO substrate, two MAO Reaction
Buffers (one that can be used with either MAO A or MAO B enzyme and
one that is designed specifically for MAO B), a lyophilized Luciferin Detection
Reagent and the Luciferin Detection Buffer. The user supplies the sample material
containing MAO. Protocols are configured for multiwell plate formats but
easily can be adapted for single-tube applications.
Features:
• Complete Solution: The MAO-Glo Assay with MAO-A contains monoamine
oxidase A enzyme for convenient assessment of the effects of new
chemical entities on MAO-A activity.
• Speed: The luminescence format eliminates the need for time-consuming
analyses such as HPLC.
• Simplified Method: The simple “add and read” protocol makes the assay
amenable to high-throughput screening in multiwell plates.
• Greater Sensitivity: Less MAO enzyme is required in these assays than in
typical HPLC or fluorometric methods because of the enhanced sensitivity.
• No Fluorescence Interference: Luminescent output eliminates interference
from fluorescent test compounds.
• Stable Signal: “Glow-type” luminescence provides a stable signal with a
half-life of greater than 5 hours. This eliminates the need for strictly timed
luminescent detection.
Storage Conditions: Store MAO-A enzyme (Cat.# V1452) at –70°C. Store all
other components at –20°C protected from light.
Protocol Part#
MAO-Glo Assay Technical Bulletin TB345
UGT Activity Assays
Product Size Cat.# Price ($)
UGT-Glo Assay 200 assays V2081 480.00
1,000 assays V2082 Pls. Enq.
UGT-Glo UGT1A1 Screening System 200 assays V2120 865.00
1,000 assays V2121 Pls. Enq.
UGT-Glo UGT2B7 Screening System 200 assays V2130 865.00
1,000 assays V2131 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The UGT-Glo Assay is a luminescent method for measuring
UDP glucuronosyltransferase (UGT) activity. The UGT-Glo Assay measures
UGT activity from a variety of sources, such as microsomes containing
recombinantly expressed enzymes or microsomal preparations derived from
mammalian tissues, and to test the effects of various chemicals on UGT activity.
The assay involves incubating UGT with a proluciferin substrate; a portion of
the substrate gets conjugated with UDP, while the remainder is unmodified.
Upon the addition of d-Cysteine, the unconjugated proluciferin is converted
into luciferin and, in a coupled reaction with luciferase/luciferin, is converted
into light. Conjugated proluciferin remains intact and does not contribute to
the luminescence. Thus, the signal generated is inversely correlated with UGT
activity present in the sample.
The UGT-Glo Assay contains two proluciferin substrates: the UGT Multienzyme
Substrate, which is compatible with a wide range of UGTs, and the
UGT1A4 Substrate, which reacts specifically with UGT1A4. The kit also contains
Luciferin Detection Reagent and Reconstitution Buffer, UGT Buffer, d-Cysteine
and UDPGA. The UGT-Glo Screening Systems contain the above reagents as
well as the respective UGT isoforms and control membranes.
Features:
• Speed: The luminescent format eliminates the need for time-consuming
analyses such as HPLC and LC/MS.
• Simplified Method: The simple “add and read” protocol makes the assay
amenable to higher throughput screening in multiwell plates.
• Sensitive: Allows researchers to use less enzyme and scale down reaction
volumes, which saves on reagent costs.
Storage Conditions: Store UGT enzymes and Control Membranes at –70°C.
Store remaining components at –20°C.
Protocol Part#
UGT-Glo Assay Technical Bulletin TB551
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