2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
274<br />
pGL2 Luciferase Reporter Vectors<br />
Product Size Cat.# Price ($)<br />
pGL2-Basic Vector 20 µg E1641 196.00<br />
pGL2-Control Vector 20 µg E1611 196.00<br />
pGL2-Enhancer Vector 20 µg E1621 196.00<br />
pGL2-Promoter Vector 20 µg E1631 196.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The pGL2 Luciferase Reporter Vectors provide a basis for the<br />
quantitative analysis of factors that potentially regulate mammalian gene<br />
expression. These factors may be cis-acting, such as promoters and enhancers,<br />
or trans-acting, such as various DNA-binding factors. The pGL2 Vectors<br />
carry the coding region for firefly (Photinus pyralis) luciferase, which is used to<br />
monitor transcriptional activity in transfected eukaryotic cells. The assay of this<br />
genetic reporter is rapid, sensitive and quantitative. In addition, the pGL2 Vectors<br />
contain numerous features that aid in the characterization and mutagenesis<br />
of the putative regulatory sequences.<br />
Features:<br />
• Versatile: Deletions and site-directed mutations can be made directly to<br />
inserted DNAs without subcloning.<br />
• Convenient: All vectors contain the firefly luciferase reporter gene, which<br />
enables sensitive and rapid quantitation of reporter activity.<br />
• Low Background: Upstream polyadenylation signal minimizes spurious<br />
transcription of the reporter gene.<br />
Storage Conditions: Store vector at –20°C. Store bacterial strain at –70°C.<br />
Protocol Part#<br />
pGL2 Luciferase Reporter Vectors Technical Manual TM003<br />
3194 SalI<br />
3188 BamHI<br />
SV40<br />
Enhancer<br />
poly(A)<br />
signal<br />
(for luc<br />
reporter)<br />
2236 PflMI<br />
SV40<br />
Amp r<br />
pGL2-Control<br />
Vector<br />
(6047bp)<br />
luc<br />
f1 ori<br />
poly(A) signal<br />
(for background<br />
reduction)<br />
SmaI<br />
KpnI<br />
SacI<br />
MluI<br />
NheI<br />
XhoI<br />
BglII<br />
SV40 Promoter<br />
HindIII 239<br />
3<br />
12<br />
18<br />
22<br />
28<br />
33<br />
37<br />
0317VA03_3A<br />
pGEM ® -luc DNA<br />
Product Size Cat.# Price ($)<br />
pGEM ® -luc DNA<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
20 µg E1541 193.00<br />
Description: The pGEM ® -luc Vector is a cassette vector designed to be a<br />
source of the luc gene encoding firefly luciferase, which is found in the pGL2<br />
Vectors. The plasmid is not intended for the expression of luciferase in eukaryotic<br />
or prokaryotic cells.<br />
The pGEM ® -luc Vector was constructed by positioning the luciferase gene<br />
(luc) in the center of the multiple cloning region of the pGEM ® -11Zf(–) Vector,<br />
providing a number of unique restriction sites at both ends of the gene. Sites<br />
that are surrounded by parentheses are not unique, as additional sites for each<br />
also exist in the luciferase gene. Note also that using HindIII or NsiI to clone<br />
the luciferase gene will include upstream ATG codons, which may reduce the<br />
efficiency of expression in eukaryotes. The luciferase cassette does not contain<br />
the prokaryotic Shine-Delgarno sequence for bacterial expression.<br />
The pGEM ® -luc Vector is supplied with a glycerol stock of bacterial strain<br />
JM109.<br />
Features:<br />
• Flexibility: Provides a luciferase cassette with several unique cloning<br />
sites at both ends for analysis of transcriptional activity, mRNA processing,<br />
protein structure/function, or labeling of cells and viruses.<br />
Storage Conditions: Store at –20°C. Store bacterial strain at –70°C.<br />
Protocol Part#<br />
pGEM ® -luc Vector Technical Bulletin<br />
TB104<br />
start<br />
1803<br />
1790<br />
1788<br />
1777<br />
1769<br />
1767<br />
1757<br />
SP6<br />
➞<br />
HindIII<br />
NsiI<br />
NotI<br />
(XbaI)<br />
ApaI<br />
BamHI<br />
Amp<br />
ori<br />
EcoNI 135<br />
r<br />
ScaI 3552 XmnI 3671<br />
AatII 3994<br />
NdeI 4243<br />
NaeI 4423<br />
DraIII 4529<br />
f1 ori<br />
lacZ′<br />
pGEM<br />
T7<br />
1 start<br />
SfiI 17<br />
SacI 27<br />
(EcoRI) 29<br />
SalI 35<br />
XhoI 41<br />
StuI 47<br />
luc<br />
BspMI 351<br />
ClaI 386<br />
EcoRV 415<br />
(XbaI) 1702<br />
PpuMI 571<br />
KasI/NarI<br />
BstEII 1141<br />
1717/1718 (EcoRI) 1162<br />
® -luc<br />
Vector<br />
(4931bp)<br />
Bsu36I 1137<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
➞<br />
0392VA05_3A
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