2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
272<br />
Chroma-Luc Vectors<br />
Product Size Cat.# Price ($)<br />
pCBR-Basic Vector 20 µg E1411 472.00<br />
pCBR-Control Vector 20 µg E1421 472.00<br />
pCBG68-Basic Vector 20 µg E1431 472.00<br />
pCBG68-Control Vector 20 µg E1441 472.00<br />
pCBG99-Basic Vector 20 µg E1451 472.00<br />
pCBG99-Control Vector 20 µg E1461 472.00<br />
For Research Use Only. Not for Use in Diagnostic Procedures.<br />
Description: The Chroma-Glo Luciferase Assay System and the<br />
Chroma-Luc Vectors can be used to generate red and green (dual-color)<br />
luminescence from a single sample upon addition of a single reagent. The<br />
Chroma-Luc Vectors consist of 6 plasmids containing synthetic versions<br />
of a red or one of two green click beetle luciferase genes; CBRluc contains a<br />
red-emitting luciferase gene, while CBG68luc and CBG99luc contain greenemitting<br />
luciferase genes. Filtered measurement of the dual-color luminescence<br />
produced by the Chroma-Luc luciferases permits each reporter<br />
to be measured independently and virtually simultaneously. Besides their<br />
different luminescence colors, the three Chroma-Luc genes differ as follows:<br />
CBG99luc and CBRluc possess 99% DNA and 98% protein homology and<br />
are the ideal choice for use when working with transient expression assays;<br />
CBG68luc and CBRluc possess 68.9% DNA homology while retaining a high<br />
degree of protein homology (>98%) and thus are the preferred pair for use<br />
with stable expression assays. Each of these genes is provided either in a Basic<br />
Vector configuration containing a multiple cloning site (MCS) or a Control Vector<br />
containing an SV40 promoter and enhancer. The Chroma-Glo Assay has a<br />
homogeneous format that generates luminescence with >30-minute signal<br />
half-lives for each of the Chroma-Luc Luciferases, thereby enabling the<br />
processing of many plates without prior sample preparation. Two reporter gene<br />
measurements can be efficiently and reproducibly determined from each well<br />
in a typical high-throughput screen.<br />
Features:<br />
• Two Reporter Signals by Single Substrate Addition: Increase your<br />
accuracy and precision through normalization, or use both reporters to<br />
multiplex experimental measurements. Use filters to spectrally separate the<br />
luminescent signals.<br />
• Ideal Control or Multiplexed Reporter System: Use the high-homology<br />
red and green luciferases to minimize potential RNA and protein effects on<br />
reporter expression.<br />
• Flexible: Use the Basic Vectors for cloning regulatory elements of interest,<br />
or use the Control Vectors as an internal control.<br />
• High Expression with Minimal Anomalous Transcription Behavior:<br />
Use the synthetic gene design to obtain results easily and reliably.<br />
Storage Conditions: Store at –20°C.<br />
Protocol Part#<br />
Chroma-Luc Reporter Vectors Technical Manual TM059<br />
A.<br />
B.<br />
SalI<br />
BamHI<br />
ori<br />
SV40 late<br />
poly(A) signal<br />
HpaI<br />
SalI<br />
BamHI<br />
SV40<br />
Enhancer<br />
SV40 late<br />
poly(A) signal<br />
HpaI<br />
ori<br />
XbaI<br />
XbaI<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
Amp r<br />
Chroma-Luc-Basic<br />
Vector<br />
Configurations<br />
Amp r<br />
Chroma-Luc-Control<br />
Vector<br />
Configurations<br />
f1 ori<br />
f1 ori<br />
Synthetic poly(A)<br />
signal/transcriptional<br />
pause site<br />
(for background<br />
reduction)<br />
KpnI<br />
SacI<br />
MluI*<br />
NheI<br />
SmaI<br />
XhoI<br />
BgIII<br />
HindIII<br />
NcoI<br />
CBRluc<br />
or<br />
CBG68luc<br />
or<br />
CBG99luc<br />
Synthetic poly(A)<br />
signal/transcriptional<br />
pause site<br />
(for background<br />
reduction)<br />
KpnI<br />
SacI<br />
MluI*<br />
NheI<br />
SmaI<br />
XhoI<br />
BgIII<br />
SV40 Promoter<br />
HindIII<br />
NcoI<br />
CBRluc<br />
or<br />
CBG68luc<br />
or<br />
CBG99luc<br />
The Chroma-Luc-Basic and -Control Vectors. These vectors contain<br />
CBRluc or CBG68luc or CBG99luc; Amp r , a gene conferring ampicillin<br />
resistance in E. coli; ori, origin of plasmid replication in E. coli. Arrows within<br />
the Chroma-Luc and Amp r genes indicate the direction of functionality.<br />
* MluI should not be used in the vector configuration containing CBG99luc, as<br />
this gene also contains the MluI site.<br />
4220MA06_3A
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