2012 Promega catalogue

Cell Signaling
Nuclear Receptor Pathway Tools
Nuclear Receptor Analysis Luciferase Vectors
Product Size Cat.# Price ($)
pGL4.36[luc2P/MMTV/Hygro] Vector 20 µg E1360 545.00
pFN26A (BIND) hRluc-neo Flexi ® Vector 20 µg E1380 545.00
pBIND-ERα Vector 20 µg E1390 545.00
pBIND-GR Vector 20 µg E1581 545.00
pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector 20 µg E1370 545.00
GloResponse 9XGAL4UAS-luc2P HEK293 Cell Line 2 vials E8530 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Nuclear receptor analysis can be performed with traditional
means by using a minimal promoter vector with nuclear receptor response
elements upstream. Alternatively, you can use viral elements like the mouse
mammary tumor virus long terminal repeat promoter to judge androgen or
glucocorticoid responses (e.g., pGL4.36). In many cases, study with these
methods requires use of a cell line with the appropriate endogenous nuclear
receptors, meaning you may need different cell lines for each nuclear receptor
study. A method using the principles of the yeast two-hybrid system was
adapted for nuclear receptor work. The nuclear receptor ligand binding domain
is fused to the GAL4 DNA binding domain and transfected with a firefly
luciferase vector containing repeats of the GAL4 upstream activation sequence
upstream of a minimal promoter. The ligand binding domain is responsible for
ligand binding, homo- or heterodimerization and interactions with co-activator
or co-repressors. The one-hybrid method allows you work with any cell line
and nuclear receptor you desire.
Features:
• Robust: GAL4-based system removes background signals from
endogenous receptors.
• More Sensitive: Optimized 9X Gal4 gives improved responses, better
signal:noise ratio.
• Adaptable: Combination Renilla/Neomycin marker allows normalization
with Dual-Luciferase ® Assay or selectable markers for generating stable
cell lines, all with one vector.
• Consistent: Compare or profile all nuclear receptors with a single
experimental system.
• Faster Results: Destabilized and optimized luc2P luciferase gene allows
greater sensitivity and shorter induction times.
Storage Conditions: Store at –20°C.
Protocol Part#
pGL4.36[luc2P/MMTV/Hygro] Vector Protocol 9PIE136
pFN26A (BIND) hRluc-neo Flexi Vector Protocol 9PIE138
pBIND-ERα Vector Protocol 9PIE139
pBIND-GR Vector Protocol 9PIE158
pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector Protocol 9PIE137
GloResponse 9XGAL4UAS-luc2P HEK293 Cell Line Technical
Bulletin
TB552
For complete and up-to-date product information visit: www.promega.com/catalog
pmirGLO Dual-Luciferase miRNA Target
Expression Vector
Product
pmirGLO Dual-Luciferase miRNA Target Expression
Size Cat.# Price ($)
Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg E1330 545.00
Description: The pmirGLO Vector is designed to quantitatively evaluate
microRNA (miRNA) activity by the insertion of miRNA target sites downstream
or 3´ of the firefly luciferase gene (luc2). Firefly luciferase is the primary
reporter gene; reduced firefly luciferase expression indicates the binding of
endogenous or introduced miRNAs to the cloned miRNA target sequence. This
vector is based on Promega dual-luciferase technology, with firefly luciferase
(luc2) used as the primary reporter to monitor mRNA regulation and Renilla luciferase
(hRluc-neo) acting as a control reporter for normalization and selection.
Features:
• Measure the function of miRNA and miRNA binding sites.
• luc2 luciferase gene provides highest expression.
• Combination Renilla/Neomycin marker allows for normalization with
Dual-Luciferase ® Assay or selectable markers for generating stable cell
lines, all with one vector.
• Moderate-strength PGK promoter provides sensitive analysis not possible
with strong promoters.
Storage Conditions: Store at –20°C.
Protocol Part#
pmirGLO Dual-Luciferase miRNA Target Expression Vector Protocol 9PIE133
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Reporter Assays and Transfection
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