2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
270
Promoterless Renilla Luciferase Vectors
Product Size Cat.# Price ($)
pGL4.70[hRluc] Vector 20 µg E6881 545.00
pGL4.71[hRlucP] Vector 20 µg E6891 545.00
pGL4.72[hRlucCP] Vector 20 µg E6901 545.00
pGL4.76[hRluc/Hygro] Vector 20 µg E6941 545.00
pGL4.77[hRlucP/Hygro] Vector 20 µg E6951 545.00
pGL4.78[hRlucCP/Hygro] Vector 20 µg E6961 545.00
pGL4.79[hRluc/Neo] Vector 20 µg E6971 545.00
pGL4.80[hRlucP/Neo] Vector 20 µg E6981 545.00
pGL4.81[hRlucCP/Neo] Vector 20 µg E6991 545.00
pGL4.82[hRluc/Puro] Vector 20 µg E7501 545.00
pGL4.83[hRlucP/Puro] Vector 20 µg E7511 545.00
pGL4.84[hRlucCP/Puro] Vector 20 µg E7521 545.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Promoterless Renilla luciferase vectors are designed primarily
to accept a putative promoter element for investigation of important regions
controlling gene transcription. Alternatively, they may be used as promoterless
control vectors in a dual-reporter system with a firefly luciferase vector serving
as the experimental vector. The promoterless vectors are available with three
varieties of engineered firefly luciferase genes: hRluc, hRlucP or hRlucCP. The
hRluc gene is engineered to remove most cryptic transcription factor binding
sites and improve mammalian expression through codon optimization. The
hRlucP and hRlucCP and RapidResponse genes are hRluc genes appended
with degradation sequences to influence the cellular half-life of the hRluc gene.
The RapidResponse genes respond more rapidly to stimuli but at the expense
of signal intensity. The hRlucP gene responds more rapidly than hRluc2
with moderate signal intensity, and the hRlucCP responds more quickly with the
lowest signal intensity. The promoterless vectors are available with or without
selectable markers (hygromycin, neomycin or puromycin).
Improved Sensitivity and Biological Relevance Due to:
• Increased Reporter Gene Expression: Codon optimization of synthetic
genes for mammalian expression.
• Reduced Background and Risk of Expression Artifacts: Removal of
cryptic DNA regulatory elements and transcription factor binding sites.
• Improved Temporal Response: Rapid Response technology available
using destabilized luciferase genes.
Additional Advantages Include:
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus
pyralis) or hRluc (Renilla reniformis) reporter genes.
• Easy Transition from Transient to Stable Cells: Choice of mammalian
selectable markers.
• Easy Transfer from Vector to Vector: Common multiple cloning site and
a unique SfiI transfer scheme.
Storage Conditions: Store at –20°C.
Signaling Pathway Analysis (Minimal
Promoter-Driven) Firefly Luciferase Vectors
Product Size Cat.# Price ($)
pGL4.23[luc2/minP] Vector 20 µg E8411 545.00
pGL4.24[luc2P/minP] Vector 20 µg E8421 545.00
pGL4.25[luc2CP/minP] Vector 20 µg E8431 545.00
pGL4.26[luc2/minP/Hygro] Vector 20 µg E8441 545.00
pGL4.27[luc2P/minP/Hygro] Vector 20 µg E8451 545.00
pGL4.28[luc2CP/minP/Hygro] Vector 20 µg E8461 545.00
pGL4.29[luc2P/CRE/Hygro] Vector 20 µg E8471 545.00
pGL4.30[luc2P/NFAT-RE/Hygro] Vector 20 µg E8481 545.00
pGL4.32[luc2P/NF-κB-RE/Hygro] Vector 20 µg E8491 545.00
pGL4.33[luc2P/SRE/Hygro] Vector 20 µg E1340 545.00
pGL4.34[luc2P/SRF-RE/Hygro] Vector 20 µg E1350 545.00
GloResponse CRE-luc2P HEK293 Cell Line 2 vials E8500 Pls. Enq.
GloResponse NFAT-RE-luc2P HEK293 Cell Line 2 vials E8510 Pls. Enq.
GloResponse NF-κB-RE-luc2P HEK293 Cell Line
For Research Use Only. Not for Use in Diagnostic Procedures.
2 vials E8520 Pls. Enq.
Description: Creating a cell line with an indicator of a functional signaling
pathway is useful for deciphering the components in a signaling pathway.
These tools are made by insertion of multiple repeats of a response element
upstream of a minimal promoter (minP). The minP vectors are available with
three varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The
luc2 gene is engineered to remove most cryptic transcription factor binding
sites and improve mammalian expression through codon optimization. The
luc2P and luc2CP and RapidResponse genes are luc2 genes appended with
degradation sequences to influence the cellular half-life of the luc2 gene. The
RapidResponse genes respond more rapidly to stimuli but at the expense of
signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than
luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour
half-life) responds more quickly with the lowest signal intensity. The minP
vectors are available with or without selectable markers (hygromycin). To speed
research, several predesigned response element vectors are available already
assembled in the pGL4.27 Vector. Some of these also are available stable cell
lines (GloResponse Cell Lines).
Improved Sensitivity and Biological Relevance Due to:
• Increased Reporter Gene Expression: Codon optimization of synthetic
genes for mammalian expression.
• Reduced Background and Risk of Expression Artifacts: Removal of
cryptic DNA regulatory elements and transcription factor binding sites.
• Improved Temporal Response: Rapid Response technology available
using destabilized luciferase genes.
Additional Advantages Include:
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus
pyralis) or hRluc (Renilla reniformis) reporter genes.
• Easy Transition from Transient to Stable Cells: Choice of mammalian
selectable markers.
• Easy Transfer from Vector to Vector: Common multiple cloning site and
a unique SfiI transfer scheme.
Storage Conditions: Store at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog