2012 Promega catalogue

Cell Signaling
Promoter-Driven Control Firefly and Renilla
Luciferase Vectors
Product Size Cat.# Price ($)
pGL4.50[luc2/CMV/Hygro] Vector 20 µg E1310 545.00
pGL4.51[luc2/CMV/Neo] Vector 20 µg E1320 545.00
pGL4.13[luc2/SV40] Vector 20 µg E6681 545.00
pGL4.73[hRluc/SV40] Vector 20 µg E6911 545.00
pGL4.74[hRluc/TK] Vector 20 µg E6921 545.00
pGL4.75[hRluc/CMV] Vector 20 µg E6931 545.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Promoter-driven Renilla control vectors are commonly
co-transfected with experimental firefly luciferase vectors for use in the
Dual-Luciferase ® or Dual-Glo ® Reporter Assay Systems. The control Renilla
vectors should give an almost invariant level of activity, while the experimental
firefly vector varies with treatment. The promoter-driven pGL4.13 firefly vector
can be used in situations where the experimental vector is designed in a Renilla
vector. The pGL4.50 and pGL4.51 are useful for tagging a cell line and offer a
selectable marker for creating stable transfectants. The pGL4.50 and pGL4.51
vectors are ideal for tagging cell lines for use in in vivo bioluminescent imaging
applications.
Improved Sensitivity and Biological Relevance Due to:
• Increased Reporter Gene Expression: Codon optimization of synthetic
genes for mammalian expression.
• Reduced Background and Risk of Expression Artifacts: Removal of
cryptic DNA regulatory elements and transcription factor binding sites.
• Improved Temporal Response: Rapid Response technology available
using destabilized luciferase genes.
Additional Advantages Include:
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus
pyralis) or hRluc (Renilla reniformis) reporter genes.
• Easy Transition from Transient to Stable Cells: Choice of mammalian
selectable markers.
• Easy Transfer from Vector to Vector: Common multiple cloning site and
a unique SfiI transfer scheme.
Storage Conditions: Store at –20°C.
Synthetic
poly(A)
Selectable
Marker
– None
– Hygror – Neor – Puror SV40 late
poly(A) signal
ori
SV40 early
enhancer/
promoter
pGL4 Vectors
Amp r
Poly(A) block
(for background
reduction)
Upstream
Element
– Multiple
cloning
region
– Promoter/
response
elements
Luciferase Gene
– Firefly (luc2)
• Rapid Response (–P, –CP)
– Renilla (hRluc)
• Rapid Response (–P, –CP)
Generic pGL4 Vector map showing the variety of genes, selectable
markers, promoters and response elements available.
For complete and up-to-date product information visit: www.promega.com/catalog
4897MA
Promoterless Firefly Luciferase Vectors
Product Size Cat.# Price ($)
pGL4.10[luc2] Vector 20 µg E6651 545.00
pGL4.11[luc2P] Vector 20 µg E6661 545.00
pGL4.12[luc2CP] Vector 20 µg E6671 545.00
pGL4.14[luc2/Hygro] Vector 20 µg E6691 545.00
pGL4.15[luc2P/Hygro] Vector 20 µg E6701 545.00
pGL4.16[luc2CP/Hygro] Vector 20 µg E6711 545.00
pGL4.17[luc2/Neo] Vector 20 µg E6721 545.00
pGL4.18[luc2P/Neo] Vector 20 µg E6731 545.00
pGL4.19[luc2CP/Neo] Vector 20 µg E6741 545.00
pGL4.20[luc2/Puro] Vector 20 µg E6751 545.00
pGL4.21[luc2P/Puro] Vector 20 µg E6761 545.00
pGL4.22[luc2CP/Puro] Vector
For Research Use Only. Not for Use in Diagnostic Procedures.
20 µg E6771 545.00
Description: Promoterless firefly luciferase vectors are designed primarily
to accept a putative promoter element for investigation of important regions
controlling gene transcription. The promoterless vectors are available with three
varieties of engineered firefly luciferase genes: luc2, luc2P or luc2CP. The
luc2 gene is engineered to remove most cryptic transcription factor binding
sites and improve mammalian expression through codon optimization. The
luc2P and luc2CP and RapidResponse genes are luc2 genes appended with
degradation sequences to influence the cellular half-life of the luc2 gene. The
RapidResponse genes respond more rapidly to stimuli but at the expense of
signal intensity. The luc2P (1-hour half-life) gene responds more rapidly than
luc2 (3-hour half-life) with moderate signal intensity, and the luc2CP (0.4-hour
half-life) responds more quickly with the lowest signal intensity. The promoterless
vectors are available with or without selectable markers (hygromycin,
neomycin or puromycin).
Improved Sensitivity and Biological Relevance Due to:
• Increased Reporter Gene Expression: Codon optimization of synthetic
genes for mammalian expression.
• Reduced Background and Risk of Expression Artifacts: Removal of
cryptic DNA regulatory elements and transcription factor binding sites.
• Improved Temporal Response: Rapid Response technology available
using destabilized luciferase genes.
Additional Advantages Include:
• Flexible Detection Options: Choice of either synthetic luc2 (Photinus
pyralis) or hRluc (Renilla reniformis) reporter genes.
• Easy Transition from Transient to Stable Cells: Choice of mammalian
selectable markers.
• Easy Transfer from Vector to Vector: Common multiple cloning site and
a unique SfiI transfer scheme.
Storage Conditions: Store at –20°C.
269
15
Reporter Assays and Transfection
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