2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
Rerorter Vectors and Cell Lines
268
pGL4 Firefly Luciferase Reporter Vectors Selection Guide
• Contains luc2 gene (Photinus pyralis):
Codon optimized for mammalian expression.
Features >90% fewer cryptic sites than luc+ (pGL3 Vectors).
• Updated Vector backbone with 78% fewer cryptic sites than pGL3.
• Improved MCR with additional RE sites.
• Available in Rapid Response Reporter configurations.
• See the online pGL4 Vector Selector at: faqs.promega.com
and choose the Solution Finder tab.
Is maximum
responsiveness
(time and fold induction)
preferred, even with lower
absolute RLUs?
YES NO
pGL4.12[luc2CP]
Vector
YES NO
pGL4.74[hRluc/TK]
Vector
pGL4.75[hRluc/CMV]
Vector
YES NO
pGL4 Renilla Luciferase Reporter Vectors Selection Guide
• Contains hRluc gene (Renilla reniformis).
• Updated Vector backbone with 78% fewer cryptic sites than pGL3/phRG.
• Available in Rapid Response Reporter configurations.
• See the online pGL4 Vector Selector at: faqs.promega.com
and choose the Solution Finder tab.
YES NO
pGL4.73[hRluc/SV40]
Vector
YES NO
pGL4.70[hRluc]
Vector
YES NO
Will you use the pGL4 Renilla Vector as a
co-reporter to normalize transfection
efficiency, cytotoxicity or other variables?
YES NO
Considerations for Choosing a Control
• Relative Light Units of the co-reporter generally should be at the lowest level necessary to
quantitate reliably. Generally speaking, the order of vector expression is pGL4.72[hRlucCP]