2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
266
ViviRen Live Cell Substrate
Product Size Cat.# Price ($)
ViviRen Live Cell Substrate 0.37 mg E6491 213.00
For Research Use Only. Not for Use in Diagnostic Procedures.
3.7 mg E6492 1372.00
37 mg E6495 Pls. Enq.
Description: ViviRen Live Cell Substrate is a uniquely engineered
coelenterazine that generates three- to fivefold brighter Renilla luciferase
luminescence than wildtype coelenterazine. Using live cells, achieve up to
100-fold higher signal-to-noise ratios for super-sensitive quantitation of
reporter gene, BRET and RNAi activity.
Cat.# E6491 is supplied as a liquid, 60mM in DMSO. Cat.# E6492 and E6495
are supplied as a lyophilized solid.
Features:
• Three- to Fivefold Brighter Renilla luminescence than coelenterazine.
Quantitate with confidence using miniaturized formats, low-level expression
and CCD imagers.
• Low Autoluminescence: Achieve unparalleled sensitivity with up to
100-fold higher signal-to-noise ratios than coelenterazine.
• Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and
RNAi applications.
• Multiplex Options: Improve accuracy and precision by combining with
CellTiter-Glo ® and other lytic assays.
Storage Conditions: Store Cat.# E6491 at –70°C. Store Cat.# E6492 and
E6495 at –20°C.
Protocol Part#
ViviRen Live Cell Substrate Technical Manual TM064
Coelenterazines
Product Size Cat.# Price ($)
Coelenterazine 250 µg S2001 199.00
Coelenterazine-h
For Research Use Only. Not for Use in Diagnostic Procedures.
250 µg S2011 204.00
Description: Luciferases from Renilla, Aequorea and other marine organisms
are commonly used as indicators or reporters for studying cellular phenomena
in expression assays in eukaryotic cells. Renilla luciferase is often used as a
reporter of transcription regulation, whereas apoaequorin is often used as a
calcium indicator. Other uses of coelenterazines include chemiluminescent
detection of Reactive Oxygen Species (ROS) in cells or tissues. Promega offers
the following coelenterazine analogs.
Coelenterazine (native) is the luminescent substrate for Renilla luciferase
and apoaequorin. Formula: C 26H 21N 3O 3. Formula Weight: 423.5. Form: Film.
Coelenterazine-h imparts a luminescent intensity with its aequorin complex
that is reported to be 10–20 times higher than that of native coelenterazine,
making this derivative a useful tool for measuring small changes in Ca 2+
concentrations. Formula: C 26H 21N 3O 2. Formula Weight: 407.5. Form: Film.
Features:
• Highly Pure: 95%.
• Custom Capabilities: Custom packaging and sizes available.
• Easy to Prepare: Supplied as a dried substrate for easy preparation in
methanol or ethanol.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
β-Galactosidase Reporter Systems
β-Galactosidase Enzyme Assay System with
Reporter Lysis Buffer
Product
β-Galactosidase Enzyme Assay System with
Size Cat.# Price ($)
Reporter Lysis Buffer
Available Separately
10 ml E2000 213.00
Reporter Lysis 5X Buffer
For Research Use Only. Not for Use in Diagnostic Procedures.
30 ml E3971 108.00
Description: The β-Galactosidase Enzyme Assay System with Reporter Lysis
Buffer is a convenient method for assaying β-galactosidase activity in lysates
prepared from cells transfected with β-galactosidase reporter vectors such as
the pSV-β-Galactosidase Control Vector.
The standard assay is performed by adding a dilute sample to an equal
volume of Assay 2X Buffer that contains the substrate ONPG (o-nitrophenylβ-d-galactopyranoside).
Samples are incubated for at least 30 minutes,
during which time the β-Galactosidase hydrolyzes the colorless substrate to
o-nitrophenyl, which is yellow. The reaction may be terminated by addition of
sodium carbonate, and the absorbance at 420nm is measured by
spectrophotometry.
Features:
• Safe: Non-isotopic assay.
• Versatile: The assay can be used in a 96-well plate format.
• Flexible: Reporter Lysis Buffer allows firefly luciferase, CAT and
β-galactosidase assays to be performed from the same cell extract.
Storage Conditions: Reporter Lysis Buffer may be stored at room
temperature. Store other system components at –20°C.
Protocol Part#
ß-Galactosidase Enzyme Assay System with Reporter Lysis Buffer
Technical Bulletin
TB097
For complete and up-to-date product information visit: www.promega.com/catalog