2012 Promega catalogue

Cell Signaling
Luminogenic Enzyme Substrates
Product Size Cat.# Price ($)
Luciferin Detection Reagent 50 ml V8921 326.00
10 ml V8920 140.00
Luciferin Detection Reagent with esterase 50 ml V8931 326.00
10 ml V8930 140.00
Luciferin-NAT2 3 mg P1721 301.00
Luciferin-3A7 3 mg P1741 301.00
Luciferin-4A 3 mg P1621 297.00
Luciferin-4F2/3 3 mg P1651 301.00
Luciferin-4F12 3 mg P1661 297.00
Luciferin-2J2/4F12 (ester) 3 mg P1671 297.00
Luciferin-MultiCYP (ester) 3 mg P1731 297.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The proluciferin substrates can be used to monitor the activity
of specific isoforms of cytochrome P450 or NAT2 as indicated in the name of
the substrate. The Luciferin-MultiCYP is a promiscuous substrate that reacts
with at least 21 P450 isoforms and is useful for measuring net CYP activity in a
mixed population of P450s. The Luciferin-NAT2 is an excellent substrate for Nacetyltransferase
2 (NAT2), a phase II biotransformation enzyme that acetylates
aromatic amine groups on xenobiotic compounds. This substrate shows little to
no cross-reactivity with NAT1.
Pgp-Glo Assay Systems
Product Size Cat.# Price ($)
Pgp-Glo Assay System 10 ml V3591 399.00
Pgp-Glo Assay System with P-glycoprotein
For Research Use Only. Not for Use in Diagnostic Procedures.
10 ml V3601 805.00
Description: The Pgp-Glo Assay Systems provide the necessary reagents
for performing luminescent P-glycoprotein (Pgp) ATPase assays. Pgp, also
known as MDR1 and ABCB1, is a 170kDa integral plasma membrane protein
that functions as an ATP-dependent drug efflux pump and plays an important
role in multidrug resistance and certain adverse drug-drug interactions. Compounds
that interact with Pgp can be identified as stimulators or inhibitors of its
ATPase activity. Compounds that are substrates for transport by Pgp typically
stimulate its ATPase activity.
The Pgp-Glo Assay detects the effects of compounds on recombinant human
Pgp in a cell membrane fraction. The assay relies on the ATP dependence of
the light-generating reaction of firefly luciferase. ATP is first incubated with Pgp;
then the Pgp ATPase reaction is stopped, and the remaining unmetabolized ATP
is detected as a luciferase-generated luminescent signal. Pgp-dependent
decreases in luminescence reflect ATP consumption by Pgp; thus the greater
the decrease in signal, the higher the Pgp activity. Accordingly, samples containing
compounds that stimulate the Pgp ATPase will have significantly lower
signals than untreated samples.
For complete and up-to-date product information visit: www.promega.com/catalog
Features:
• Complete System: Cat.# V3591 includes all the reagents required to run
the assay except the P-glycoprotein: Pgp reaction buffer, MgATP, Verapamil,
Na 3VO 4, and a lyophilized ATP detection reagent and its reconstitution
buffer. Cat.# V3601 includes all the reagents provided in the Pgp-Glo
System with the addition of Recombinant Human Pgp Membranes to
provide a completely optimized kit.
• Stable Activities: “Glow-type” signal allows processing of multiple
samples without concern of variability over time.
• Low False-Positive Rate: Use of a proprietary stabilized firefly luciferase
and a proprietary luciferase assay formulation minimizes the incidence of
false positives due to inhibition of luciferase by analytes when screening for
compounds that affect Pgp activity.
• Simple: The simple protocol makes the assay amenable to high-throughput
screening in multiwell plates.
Storage Conditions: Store Recombinant Human Pgp Membranes at –70°C.
All other components can be stored at –70°C or –20°C, protected from light.
Protocol Part#
Pgp-Glo Assay Systems Technical Bulletin TB341
23
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Cell Health Assays
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