2012 Promega catalogue

Cell Signaling
EnduRen Live Cell Substrate
Product Size Cat.# Price ($)
EnduRen Live Cell Substrate 0.34 mg E6481 213.00
For Research Use Only. Not for Use in Diagnostic Procedures.
3.4 mg E6482 1372.00
34 mg E6485 Pls. Enq.
Description: EnduRen Live Cell Substrate provides new capabilities in
performing luminescent reporter assays by enabling live cell kinetic measurements,
streamlining assay development and multiplexing with other lytic
assays. EnduRen Live Cell Substrate provides the ability to measure Renilla
luciferase luminescence for at least 24 hours after substrate addition, with up
to tenfold higher signal-to-background ratios than wildtype coelenterazines.
EnduRen Live Cell Substrate is a uniquely engineered coelenterazine with
protected oxidation sites, which minimizes substrate degradation and
autoluminescence (background) in cell culture, while it extends the luminescent
signal to accommodate microplates without the need for auto-injectors. The
result is that EnduRen Live Cell Substrate overcomes the key limitations of
wildtype coelenterazines by providing an automation-friendly, highly sensitive
substrate for Renilla luciferase-based gene reporter and BRET applications.
Features:
• Live Cell Assay: Generate kinetic profiles for reporter gene, BRET and
RNAi applications.
• Kinetic Reporter Gene Analysis: Conserve test compounds as you
create response profiles in real time to generate more data-rich results.
• Streamlined Assay Development and Screening: Rapidly obtain
optimal assay parameters through repeat measurements using only a
single cell population. Increase your sample throughput using microplates
without time-consuming per-sample reagent injection steps.
• Designed for Multiplexing: Perform more dynamic experiments using
the same sample set by pairing with any lytic assay.
• High Signal-to-Background Ratios: Reliably quantitate low levels of
expression for reporter gene detection and BRET.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –20°C.
Protocol Part#
EnduRen Live Cell Substrate Technical Manual TM244
Isoproterenol
(6µM)
For complete and up-to-date product information visit: www.promega.com/catalog
Fold Induction
12
10
8
6
4
2
Forskolin
(10µM)
0
0 6 12 18 24 30 36
Time (hours)
Real-Time live-cell reporter assay using the EnduRen Substrate.
Luminescence was monitored from HEK 293 cells for >24 hours, permitting
measurement of the effects of sequential treatment of the cells with
isoproterenol and forskolin.
4273MA08_3A
265
15
Reporter Assays and Transfection
Section
Contents
Table of
Contents