2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
264
Beetle Luciferin, Potassium Salt
Product Size Cat.# Price ($)
Beetle Luciferin, Potassium Salt 5 mg E1601 125.00
For Research Use Only. Not for Use in Diagnostic Procedures.
50 mg E1602 607.00
250 mg E1603 1771.00
1 g E1605 Pls. Enq.
Description: Luciferase genes from the North American firefly (Photinus
pyralis) and from other beetles are commonly used as reporter genes for
studying transcription regulation in transient assay systems and as markers for
stably transformed eukaryotic cells. Beetle luciferin (also known as d-luciferin)
is synthesized as the monopotassium salt and is a substrate for the beetle
luciferase reporter systems. d-luciferin is provided for those researchers who
prefer to formulate their own assay reagents for monitoring in vitro or in vivo
luciferase activity.
Formula: C 11H 7N 2O 3S 2·K.
Formula Weight: 318.4Da (anhydrous).
Features:
• Formulation: Supplied as a potassium salt for easy preparation in
aqueous buffer.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store at –70°C.
Luciferin-EF Endotoxin-Free Luciferin Na
Product Size Cat.# Price ($)
Luciferin-EF 25 mg E6551 460.00
For Research Use Only. Not for Use in Diagnostic Procedures.
250 mg E6552 1936.00
Description: Luciferin-EF is an endotoxin-free beetle luciferin that can be
used for cell-based imaging applications in living systems, where endotoxin
may create problems. Luciferin-EF is tested to ensure endotoxin is below
detectable levels and packaged in amber vials with septa to facilitate easy
dilution and use.
Features:
• Achieve Endotoxin Levels Below Detection Limits: No potential
interference in assay due to the presence of endotoxins.
• Be Assured of Product Integrity: Luciferin-EF is packaged in amber
vials with septa to ensure product integrity as well as offer ease of dilution
and use for imaging experiments.
• Appreciate Flexibility and Convenience: Luciferin-EF is available in
two sizes, depending on the number of experiments to be performed.
Storage Conditions: Store at –70°C.
Renilla Luciferase Reporter Systems
Renilla-Glo Luciferase Assay System
Product Size Cat.# Price ($)
Renilla-Glo Luciferase Assay System 10 ml E2710 301.00
100 ml E2720 1659.00
10 × 100 ml E2750 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Renilla-Glo Luciferase Assay System is a single-addition
reagent that generates a glow-type signal with Renilla luciferase. When
reconstituted, it has the capacity to lyse cells, reduce the autoluminescence of
the coelenterazine substrate, and produce a stable signal (i.e., half-life greater
than 60 minutes at 22°C).
Features:
• Simplify Your Assay Optimization: Add-and-read simplicity for a Renilla
luciferase reporter system.
• Improve Assay Precision: No need for separate lysis and reagent
injection steps.
• Get a Brighter, Longer-Lasting Signal: Extended bright light output is
optimized for batch and continuous-process handling.
• Reduced Autoluminescence: Low background formulation offers
increased sensitivity.
Storage Conditions: Store at –20°C.
Protocol Part#
Renilla-Glo Luciferase Assay System Technical Manual TM329
Renilla Luciferase Assay System
Product Size Cat.# Price ($)
Renilla Luciferase Assay System 100 assays E2810 274.00
1,000 assays E2820 1508.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: Renilla Luciferase Assay System is designed to provide a fast
and sensitive method of detecting the luciferase from sea pansy (Renilla
reniformis). The system is a convenient alternative to firefly (Photinus pyralis)
reporter systems and is designed to yield reliable, linear results for a
concentration range over 7 orders of magnitude. The Renilla Luciferase Assay
System has been formulated with a proprietary composition that significantly
reduces the effect of coelenterazine autoluminescence when compared to
other reagents, making the reagent orders of magnitude more sensitive than
published methods. This system enables measurements with wildtype and
the synthetic hRluc genes for primary expression or internal normalization
measurements of gene expression.
Features:
• Reduced Autoluminescence: Low background, increased sensitivity.
• Sensitive: 10 –19 moles of Renilla luciferase detectable.
• Linear: Linear range extending 7 logs.
• Unique: The first independent assay system for Renilla luciferase.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store the Renilla Luciferase Assay System at –20°C.
Protocol Part#
Renilla Luciferase Assay System Technical Manual TM055
For complete and up-to-date product information visit: www.promega.com/catalog