2012 Promega catalogue

Cell Signaling
Bright-Glo Luciferase Assay System
Product Size Cat.# Price ($)
Bright-Glo Luciferase Assay System 10 ml E2610 239.00
For Laboratory Use.
100 ml E2620 1879.00
10 × 100 ml E2650 Pls. Enq.
Description: High-throughput quantitation of firefly (Photinus pyralis)
luciferase expression in mammalian cells requires highly sensitive reagents
that can adapt to continuous-process robotic systems. Bright-Glo Luciferase
Assay System is designed specifically to meet the needs of continuous-process
systems by providing robust, homogeneous assay chemistry that achieves high
assay sensitivity and approximately 30-minute signal half-life without prior
sample processing. These attributes also benefit scientists who are using fewer
samples but still require high sensitivity and ease of use.
Features:
• Quick: No need to remove culture medium or wash cells prior to adding
assay reagent. Cells may be grown and assayed directly within the same
multiwell plate.
• Sensitive: Up to tenfold more light intensity than other homogeneous
luciferase assay reagents results in increased sensitivity.
• Improved Assay Precision: Less sensitive to mixing conditions, sample
evaporation and pipetting errors, resulting in improved assay
reproducibility.
• Convenient: Simply mix buffer with lyophilized substrate and add to cells
in culture medium; no need for thawing or measuring before use.
• Choose Your Configuration: Learn more about our custom options for
this product at: www.promega.com/myway/
Storage Conditions: Store Bright-Glo Luciferase Assay Substrate at
–20°C. Store Bright-Glo Luciferase Assay Buffer below 25°C.
Protocol Part#
Bright-Glo Luciferase Assay System Technical Manual TM052
Glo Lysis Buffer, 1X
Product Size Cat.# Price ($)
Glo Lysis Buffer, 1X
For Research Use Only. Not for Use in Diagnostic Procedures.
100 ml E2661 136.00
Description: Glo Lysis Buffer (GLB), 1X, is a proprietary formulation developed
to promote rapid lysis (within 5 minutes) of cultured mammalian cells without
scraping or performing freeze-thaw cycles. It is fully compatible with Bright-
Glo and Steady-Glo ® Assay Reagents and the Luciferase Assay Reagent for
analysis of firefly luciferase expression. The half-life of these reagents remains
the same with or without use of GLB, >5 hours for Steady-Glo ® Reagent and
>24 minutes for Bright-Glo Reagent.
Features:
• Convenient: No need for cell scraping or freeze-thaw cycles.
• Fast: Cell lysis within 5 minutes.
• Versatile: Use with Bright-Glo and Steady-Glo ® Reagents to provide
nonhomogeneous assay formats or with other reporter applications.
• Robust: Firefly luciferase enzyme in Glo Lysis Buffer is stable at room
temperature for at least 48 hours.
Storage Conditions: Store Glo Lysis Buffer at 4°C. For long-term storage,
Glo Lysis Buffer can be frozen at –20°C or –70°C.
Protocol Part#
Glo Lysis Buffer Protocol 9PIE266
For complete and up-to-date product information visit: www.promega.com/catalog
Luciferase Assay System
Product Size Cat.# Price ($)
Luciferase Assay System
Luciferase Assay System with Reporter Lysis
100 assays E1500 190.00
Buffer 100 assays E4030 242.00
Luciferase Assay System, 10-Pack 1,000 assays E1501 1152.00
Luciferase Assay System Freezer Pack 1,000 assays E4530 1942.00
Luciferase 1000 Assay System 1,000 assays E4550 1152.00
Luciferase Assay Reagent
Available Separately
100 ml E1483 1199.00
Luciferase Cell Culture Lysis 5X Reagent 30 ml E1531 76.00
Reporter Lysis 5X Buffer 30 ml E3971 108.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Luciferase Assay System is an extremely sensitive and rapid
reagent for quantitation of firefly luciferase. Linear results are seen over at least
eight orders of magnitude of enzyme concentration, and patented
technology incorporated in the formulation has allowed for less than 10 –20
moles of luciferase to be measured under optimal conditions. Generally,
100-fold greater sensitivity can be achieved over the chloramphenicol
acetyltransferase (CAT) assay. The Luciferase Assay Reagent generates
light that is nearly constant for at least 1 minute and so is compatible with
measuring firefly luciferase in a single-tube luminometer or in a multiwell plate
luminometer with an auto-injector.
The Luciferase Assay System is a nonhomogeneous assay system; the cells
containing the luciferase must be lysed before reagent addition. Glo Lysis
Buffer (Cat.# E2661), Cell Culture Lysis Reagent (Cat.# E1531), Passive Lysis
Buffer (Cat.# E1941) and Reporter Lysis Buffer (Cat.# E3971) may be used with
the Luciferase Assay System for reporter quantitation in mammalian cells. The
Luciferase Assay System may also be used for quantitation in plant and bacterial
cells, but only Cell Culture Lysis Reagent is suitable for these applications.
Reporter Lysis Buffer allows for firefly luciferase, CAT and β-galactosidase
assays to be performed from the same cell extract. In some kits the lysis buffer
is included, and in others it must be purchased separately (see Component
Listing link above).
Features:
• Linear: Eight or more orders of magnitude of enzyme concentration.
• Sensitive: To 10 –20 moles of luciferase.
• Fast: Perform cell lysis, sample preparation and assays in as little as
5 minutes.
• Convenient: Reporter Lysis Buffer allows luciferase, CAT and
β-galactosidase assays to be performed from the same cell extract.
• Simple Assay Procedure: Eliminates the need for autoinjection devices
and rapid mixing protocols when using single-tube luminometers.
• Versatile: Luminometer preferred, but not required; adaptable to
scintillation counters.
• Safe: Non-radioactive.
• Superior: High performance compared to competitors’ luciferase assays.
Storage Conditions: Store system at –20°C. Store Cat.# E1483 at –70°C.
Reporter Lysis Buffer (Cat.# E3971) may be stored at room temperature. Store
Cat.# E2661 at 4°C. For long-term storage, Cat.# E2661 can be frozen at
–20°C or –70°C.
Protocol Part#
Luciferase Assay System Technical Bulletin TB281
263
15
Reporter Assays and Transfection
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