2012 Promega catalogue
2012 Promega catalogue
2012 Promega catalogue
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Life<br />
Science<br />
Catalog<br />
<strong>2012</strong><br />
Worldwide Contact List<br />
Section<br />
Contents<br />
Table of<br />
Contents<br />
Cell Signaling<br />
Dual-Reporter Systems<br />
Genetic reporters can<br />
be used in a wide<br />
variety of different<br />
applications. Luciferase<br />
makes an ideal reporter<br />
with great sensitivity,<br />
ease of use and<br />
quantitation.<br />
Is limit of detection the critical<br />
requirement, even at the expense<br />
of throughput and ease of use?<br />
YES NO<br />
Dual-<br />
Luciferase ®<br />
Reporter<br />
Assay System<br />
258<br />
Glo<br />
NO<br />
Dual-Glo<br />
Luciferase<br />
Assay System<br />
External Stimuli<br />
Viral Infection<br />
and Propagation<br />
To view an animation about<br />
Bioluminescence Assays go to:<br />
www.promega.com/multimedia/<br />
Protein Folding<br />
Genetic Reporters Selection Guide<br />
Protein-Protein<br />
Interactions<br />
• Are you performing transient transfection assays?<br />
• Are you performing an assay that is designed to result in the decrease of signal, as in those that downregulate cellular<br />
responses to stimuli?<br />
• Do you require a co-reporter for establishing an internal control or for multiplexing?<br />
• Is achieving the highest assay precision (low inter- and intraplate variability) a critical requirement?<br />
• Is a single reagent addition for higher<br />
throughput important to your work?<br />
• Is your application volume restricted, such<br />
that less reagent volume must be added?<br />
NO<br />
EnduRen Live<br />
Cell Substrate<br />
+ Steady-Glo ®<br />
Luciferase<br />
Assay System<br />
Promoter luc<br />
Translation<br />
If YES to Any<br />
Dual-Reporter Systems Single-Reporter Systems<br />
YES<br />
Receptor<br />
mRNA Processing<br />
siRNA<br />
• Do you have an instrument capable of filtered<br />
measurements in luminescence mode?<br />
• Do you require genetically similar, dual-color<br />
reporters?<br />
YES<br />
Chroma-Glo<br />
Luciferase<br />
Assay System<br />
Signal<br />
Transduction<br />
Transcription<br />
Factors<br />
Transcription<br />
Ribozyme<br />
Bright-Glo<br />
Luciferase<br />
Assay System<br />
Cytoplasm<br />
Nucleus<br />
Promoter luc Glo<br />
Are sample throughput<br />
and ease of use<br />
critical requirements?<br />
YES NO<br />
Is luminescence intensity a<br />
more critical requirement<br />
than signal stability?<br />
YES NO<br />
Which reporter constructs<br />
do you wish to use?<br />
Firefly Renilla β-gal<br />
Steady-Glo ®<br />
Luciferase<br />
Assay System<br />
Promoter luc Glo<br />
Deletion Analysis<br />
EnduRen<br />
Live Cell<br />
Substrate<br />
If NO to All<br />
Is limit of detection the<br />
critical requirement,<br />
even at the expense of<br />
throughput and ease of use?<br />
Beta-Glo ®<br />
Assay System<br />
Renilla<br />
Luciferase<br />
Assay System<br />
Luciferase<br />
Assay System<br />
For complete and up-to-date product information visit: www.promega.com/catalog<br />
NO<br />
YES<br />
Which reporter construct<br />
do you wish to use?<br />
Renilla Firefly<br />
This chart and the chart on page 141 provide a general guide for selecting from <strong>Promega</strong> luminescence reporter assays and firefly and Renilla luciferase genes.<br />
These charts contain general questions about your application needs and preferences and are designed to recommend a product based on your answer(s).<br />
Selection of a particular reporter assay or gene may require additional considerations and preferences not addressed by these charts. In these instances, please<br />
contact Technical Services for assistance.<br />
5678MH<br />
4311MA09_3B
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