2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
22
P450-Glo CYP450 Screening Systems
Product Size Cat.# Price ($)
P450-Glo CYP3A4 Screening System with
Luciferin-IPA
P450-Glo CYP3A4 Screening System
(Luciferin-PPXE)
1,000 assays V9920 1455.00
DMSO-Tolerant Assay 1,000 assays V9910 1455.00
P450-Glo CYP1A2 Screening System 1,000 assays V9770 1455.00
P450-Glo CYP2C9 Screening System 1,000 assays V9790 1455.00
P450-Glo CYP3A4 Screening System 1,000 assays V9800 1455.00
P450-Glo CYP2C19 Screening System 1,000 assays V9880 1455.00
P450-Glo CYP2D6 Screening System
Available Separately
1,000 assays V9890 1455.00
NADPH Regeneration System 1,000 assays V9510 180.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The P450-Glo Screening Systems provide a complete set
of reagents for performing luminescent cytochrome P450 (CYP450) assays.
The systems include a membrane preparation containing recombinant human
CYP450 enzyme, a luminogenic CYP450 substrate appropriate for the enzyme,
an NADPH Regeneration System, reaction buffer, Luciferin Detection Reagent
and Luciferin-Free Water. The membranes are prepared from baculovirusinfected
insect cells and contain human CYP450 and P450 reductase (and
cytochrome b5 for CYP2C9 and CYP3A4). The P450-Glo Screening Systems
also contain a membrane fraction devoid of cytochrome P450 activity as a
negative control. The assays are ideal for testing the effects of drugs and new
chemical entities on cytochrome P450 enzyme activities.
The cytochrome P450 reaction is performed by incubating a luminogenic
CYP450 substrate with a CYP450 enzyme and the NADPH Regeneration System.
The luminogenic P450-Glo Substrates are derivatives of beetle
luciferin ((4S)-4,5-dihydro-2-(6-hydroxybenzothiazolyl)-4-thiazolecarboxylic
acid or d-luciferin), a substrate of firefly luciferase. The P450-Glo Substrates
do not react with luciferase but are converted by cytochrome P450 to luciferin,
which in turn reacts with luciferase to produce light. Light is used to monitor
CYP450 activity because the amount of light produced is directly proportional
to the amount of d-luciferin produced by cytochrome P450.
The new P450-Glo CYP3A4 Screening System with Luciferin-IPA
contains our latest 3A4 substrate and is the most sensitive P450-Glo 3A4
substrate available. Luciferin-IPA displays the widest range of inhibition profiles
of all the P450-Glo CYP3A4 substrates.
Dimethyl sulfoxide (DMSO) can inhibit the activity of the 3A4 isoform of
CYP450, even at low concentrations (