2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
254
HaloLink Protein Array Systems
Product Size Cat.# Price ($)
HaloLink Array (TnT ® T7 Quick) Two Slide
System 2 arrays G6140 649.00
HaloLink Array (TnT ® SP6 Wheat Germ) Two
Slide System 2 arrays G6180 649.00
HaloLink Array Six Slide System 6 slides G6190 Pls. Enq.
HaloTag ® Standard Protein 30 µg G4491 89.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 247.
HaloLink Magnetic Beads
Product Size Cat.# Price ($)
HaloLink Magnetic Beads 40 reactions G9311 169.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 248.
HaloCHIP System
Product Size Cat.# Price ($)
HaloCHIP System 20 reactions G9410 829.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 249.
HaloLink Resin
Product Size Cat.# Price ($)
HaloLink Resin 1.25 ml G1912 509.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 246.
2.5 ml G1913 201.00
10 ml G1914 464.00
25 ml G1915 850.00
HaloTag ® Mammalian Pull-Down Systems
Product Size Cat.# Price ($)
HaloTag ® Mammalian Pull-Down and
Labeling System 24 reactions G6500 521.00
HaloTag ® Mammalian Pull-Down System 24 reactions G6504 284.00
HaloTag ® Control Vector 20 µg G6591 231.00
Available Separately
Protease Inhibitor Cocktail, 50X 1 ml G6521 87.00
Mammalian Lysis Buffer
For Research Use Only. Not for Use in Diagnostic Procedures.
40 ml G9381 58.00
For additional information see page 245.
MagneGST Pull-Down System
Product Size Cat.# Price ($)
MagneGST Pull-Down System 80 reactions V8870 913.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 250.
Gel Shift Assay Systems
Product Size Cat.# Price ($)
Gel Shift Assay Core System 100 reactions E3050 592.00
Gel Shift Assay System
Available Separately
HeLaScribe
100 reactions E3300 767.00
® Nuclear Extract, Gel Shift Assay
Grade 3 × 40 µl E3521 317.00
Gel Shift Binding 5X Buffer 5 × 200 µl E3581 487.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The gel shift or electrophoretic mobility shift assay provides
a simple and rapid method for detecting DNA-binding proteins. This method
is widely used to study sequence-specific DNA-binding proteins such as
transcription factors. The assay is based on the observation that complexes of
protein and DNA migrate through a nondenaturing polyacrylamide gel more
slowly than free DNA fragments or double-stranded oligonucleotides. The gel
shift assay is performed by incubating a purified protein or a complex mixture
of proteins (such as nuclear or cell extract preparations) with a 32 P end-labeled
DNA fragment containing the putative protein binding site. The reaction products
are then analyzed on a nondenaturing polyacrylamide gel. The specificity
of the DNA-binding protein for the putative binding site is established by
competition experiments using unlabeled DNA fragments or oligonucleotides
containing a binding site for the protein of interest or other unrelated DNA
sequences.
The Core System (Cat.# E3050) includes HeLa Nuclear Extract and SP1 and
AP2 Consensus Oligos that can be used as positive controls and serve as a
reliable system for obtaining experience with gel shift assays. In addition, the
Core System contains T4 Polynucleotide Kinase and Kinase 10X Buffer for
labeling oligonucleotides as well as Gel Shift Binding 5X Buffer. Cat.# E3300
contains all of the above plus consensus oligos for AP1, OCT1, CREB, NF-κB,
and TFIID.
Features:
• Positive Controls: The Gel Shift Assay Core System includes a HeLa
Nuclear Extract and consensus oligonucleotides for AP2 and SP1.
• Versatile: Oligonucleotides can be 5´ end-labeled and used as proteinspecific
probes or used as unlabeled oligonucleotides in competition
assays.
Storage Conditions: Store HeLa Nuclear Extract at –70°C. Store other
components at –20°C.
Protocol Part#
Gel Shift Assay System Technical Bulletin TB110
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