2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 250 FastBreak Cell Lysis Reagent Product Size Cat.# Price ($) FastBreak Cell Lysis Reagent, 10X 15 ml V8571 53.00 For Research Use Only. Not for Use in Diagnostic Procedures. 60 ml V8572 150.00 100 ml V8573 233.00 Description: FastBreak Cell Lysis Reagent is designed for the efficient, gentle lysis of E. coli cultures without the need for centrifugation or mechanical cell disruption. The reagent is provided as a 10X concentrate and contains a proprietary nonionic detergent to facilitate lysis. Add the reagent directly to E. coli cultures. Following a brief incubation, the cells are disrupted, and the protein of interest is released. Recombinant proteins can be directly screened in the cell extract or purified by the addition of the appropriate affinity matrix such as the MagneHis Protein Purification System. This product is suitable for both manual and automated protocols. Features: • Save Time: Eliminate centrifugation or mechanical disruption. • Easy to Use: Add and incubate. • Flexible: Use manually or on a robotic platform. Storage Conditions: Store at 4–25°C. Protocol Part# FastBreak Cell Lysis Reagent Product Information 9PIV857 GST Protein Purification MagneGST Protein Purification System Product Size Cat.# Price ($) MagneGST Protein Purification System 40 reactions V8600 245.00 Available Separately 200 reactions V8603 843.00 MagneGST Glutathione Particles 4 ml V8611 187.00 20 ml V8612 682.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneGST Protein Purification System provides a simple, rapid and reliable method for the purification of glutathione-S-transferase (GST) fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST Particles) are used to isolate GST-tagged protein directly from a crude or cleared lysate using either a manual or automated procedure and requires use of a magnetic stand. GST-tagged proteins can be purified on a small scale from 1ml of culture or on a large scale using more than 50ml of culture. Samples also can be processed using a robotic platform. Features: • Simple: One-step purification of multiple samples with easy handling. • Quick: After cell lysis, no requirement for high-speed centrifugation to clear lysate. • Scalable: Scalable protocol using 1–50ml of cell culture. • Efficient: Achieve high yields with little or no nonspecific background. • Complete: System contains all necessary components except magnetic stand. Storage Conditions: The complete system consists of two individual parts, each with a different storage condition. Store individual boxes at specified temperatures of 4°C and –70°C. Protocol Part# MagneGST Protein Purification System Technical Manual TM240 MagneGST Pull-Down System Product Size Cat.# Price ($) MagneGST Pull-Down System 80 reactions V8870 913.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagneGST Pull-Down System is designed for detection of protein interactions between GST-fusion proteins expressed in bacterial lysates and prey proteins expressed in the TnT ® Systems. Prey protein synthesized in the TnT ® Quick Coupled Transcription/Translation Reaction is captured using bait protein (GST-fusion protein) immobilized on MagneGST Particles. Nonspecifically bound proteins are then washed away, and the prey protein is analyzed. Prey proteins can be detected by incorporating radioactively labeled methionine in the TnT ® Quick reaction, followed by SDS-PAGE and autoradiography or by incorporating the supplied non-radioactive methionine in the TnT ® reaction and detecting by Western blotting with protein-specific antibodies. Storage Conditions: Store the TnT ® T7 Quick Master Mix and Methionine at –70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free Water, MagneGST Glutathione Particles, MagneGST Binding/Wash Buffer and Cell Lysis Reagent at 4°C. Protocol Part# MagneGST Pull-Down System Technical Manual TM249 Polyhistidine His-Tag Protein Purification Maxwell ® 16 Polyhistidine Protein Purification Kit Product Size Cat.# Price ($) Maxwell ® 16 Polyhistidine Protein Purification Kit 48 preps AS1060 Pls. Enq. For Research Use Only. Not for Use in Diagnostic Procedures. Description: The Maxwell ® 16 Polyhistidine Protein Purification Kit is used with the Maxwell ® 16 Instrument to provide an easy method for the efficient, automated purification of polyhistidine-tagged protein from bacterial cultures and other sample types including mammalian and insect cells. With minor modifications, the reagents can also be used for purification of HQ-tagged proteins from bacterial cultures. The Maxwell ® Instrument is supplied with a preprogrammed purification procedure and reagent cartridges specifically designed to maximize simplicity and convenience. The instrument can process up to 16 samples in approximately 40 minutes. The purified protein is compatible with downstream applications such as gel electrophoresis and Western blot analysis. Features: • Choose Your Sample Type: Flexibility to purify from multiple starting cultures including bacterial culture, mammalian cells, insect cells and culture medium. • Have Confidence in Your Results: Achieve consistent purification across all samples. • Save Hands-On Time: Prefilled cartridges eliminate reagent preparation, multiple pipetting steps, centrifugation and additional sample manipulation. Storage Conditions: Store at 4°C. Protocol Part# Maxwell ® 16 Polyhistidine Protein Purification Kit Technical Manual TM285 For complete and up-to-date product information visit: www.promega.com/catalog
Cell Signaling MagneHis Protein Purification System Product Size Cat.# Price ($) MagneHis Protein Purification System 65 reactions V8500 297.00 Available Separately 325 reactions V8550 1056.00 MagneHis Ni-Particles 2 ml V8560 231.00 For Research Use Only. Not for Use in Diagnostic Procedures. 10 ml V8565 815.00 Description: The MagneHis Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins. Paramagnetic precharged nickel particles (MagneHis Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format, polyhistidineor HQ-tagged protein can be purified on a small scale using less than 1ml of culture or on a large scale using more than 1 liter of culture. Samples can be processed in a high-throughput manner using a robotic platform such as the Beckman Coulter Biomek ® 2000 or FX. Features: • Simple: No centrifugation or vacuum is required once the cells are lysed. • Flexible: MagneHis Ni-Particles are compatible with a variety of common buffers. • Quick: No long incubations with lysozyme are required for cell lysis. • Scalable: Volumes can be adjusted to correspond to the amount of material to be purified, 1ml to 1 liter of cell culture. • Efficient: Binding capacity is up to 1mg of polyhistidine-tagged protein per 1ml of MagneHis Ni-Particles. • Versatile: Purification can be performed manually (requires use of a magnetic stand) or using an automated platform. • Convenient: Complete system that includes all necessary components, including a unique lysis buffer. • Automate This Assay: Validated automated methods are available for Beckman Coulter Biomek ® 2000 and FX and Tecan Genesis ® RSP at: www.promega.com/automethods/ Storage Conditions: Store at 4°C. Protocol Part# MagneHis Protein Purification System Technical Manual TM060 MagZ Protein Purification System Product Size Cat.# Price ($) MagZ Protein Purification System 30 reactions V8830 376.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The MagZ Protein Purification System provides a simple, rapid and reliable method for the purification of expressed polyhistidine- or HQ-tagged proteins, which are 99% free of hemoglobin contamination, from rabbit reticulocyte lysate. Based on the use of proprietary, paramagnetic precharged particles, polyhistidine- or HQ-tagged protein can be isolated from 50–500μl of TnT ® Coupled Transcription/Translation reactions. Polyhistidine- or HQ-tagged proteins bind to the particles in minutes, while unbound proteins are washed away, and the target protein is eluted with imidazole. Features: • Specific: Minimal hemoglobin (20mmol Ni/ml settled resin). The resin is designed to efficiently capture and purify bacterially expressed polyhistidine- or HQ-tagged proteins. The HisLink Resin also may be used for general applications requiring an immobilized metal affinity chromatography (IMAC) matrix. HisLink Resin may be used in either column or batch purification formats. The lysate flows by gravity over a column of packed HisLink Resin at a rate sufficient for complete capture and efficient elution of polyhistidine- or HQ-tagged proteins from cleared lysate. The resin may be used in vacuum filtration devices (e.g., Vac-Man ® Vacuum Manifold [Cat.# A7231]), allowing simultaneous processing of multiple columns. HisLink Resin is also an excellent choice for affinity purification of polyhistidine- or HQ-tagged proteins with low- to medium-pressure liquid chromatography systems such as FPLC. In batch format, HisLink Resin may be separated easily from the lysate without filtration (or centrifugation), allowing processing of larger quantities of lysate and the ability to purify protein without clearing the lysate of insoluble cellular debris. Features: • Optimize Yields: High binding capacity (>15mg/ml). • Save Time: Purify polyhistidine- or HQ-tagged proteins from cleared or crude cell lysates. • Flexible: Use standard gravity column chromatography or automated applications such as FPLC. Storage Conditions: Store at 4°C. Protocol Part# HisLink Protein Purification Resin Technical Bulletin TB327 HisLink 96 Protein Purification System Product Size Cat.# Price ($) HisLink 96 Protein Purification System 1 × 96 blank V3680 462.00 5 × 96 blank V3681 1950.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: HisLink 96 Protein Purification System provides a simple, quick and robust method of purifying multiple polyhistidine- or HQ-tagged expressed proteins from E. coli using a vacuum-based method. The system is designed to purify expressed polyhistidine- or HQ-tagged proteins directly from deep-well, 96-well plates. The HisLink 96 System is amenable to manual or automated methods, such as the Beckman Coulter Biomek ® 2000 or FX for high-throughput applications. Using the provided FastBreak Cell Lysis Reagent, bacterial cells containing polyhistidine- or HQ-tagged protein are lysed directly in culture. The HisLink Resin is added directly to the lysate, and the polyhistidine- or HQ-tagged proteins bind in a matter of minutes. Samples are then transferred to a filtration plate, unbound proteins are washed away and the target protein is recovered by elution with imidazole. Features: • Simple: No centrifugation required; lysis buffer is added directly to cells in culture medium. • Quick: No long lysozyme incubations are required for cell lysis. • Versatile: Perform purification manually or on an automated platform. • Efficient: Binding capacity of 1mg of polyhistidine-tagged protein per well. Storage Conditions: Store the system at 4°C. Plates may be stored at 4°C or room temperature. Protocol Part# HisLink 96 Protein Purification System Technical Bulletin TB342 251 14 Protein Purification and Interactions Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Cell Signaling Maxwell ® 16 System
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Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
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Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
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Page 227 and 228: Cell Signaling TnT ® Quick Coupled
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Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
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Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
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Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
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Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253: Cell Signaling HaloCHIP System Prod
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
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Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291 and 292: Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294: Cell Signaling RNA Interference Gen
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Page 297 and 298: Cell Signaling Cell ID System Produ
Page 299 and 300: Cell Signaling StemElite Human Panc
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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2012 Promega catalogue

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