2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
250
FastBreak Cell Lysis Reagent
Product Size Cat.# Price ($)
FastBreak Cell Lysis Reagent, 10X 15 ml V8571 53.00
For Research Use Only. Not for Use in Diagnostic Procedures.
60 ml V8572 150.00
100 ml V8573 233.00
Description: FastBreak Cell Lysis Reagent is designed for the efficient,
gentle lysis of E. coli cultures without the need for centrifugation or mechanical
cell disruption. The reagent is provided as a 10X concentrate and contains a
proprietary nonionic detergent to facilitate lysis. Add the reagent directly to
E. coli cultures. Following a brief incubation, the cells are disrupted, and the
protein of interest is released. Recombinant proteins can be directly screened
in the cell extract or purified by the addition of the appropriate affinity matrix
such as the MagneHis Protein Purification System. This product is suitable
for both manual and automated protocols.
Features:
• Save Time: Eliminate centrifugation or mechanical disruption.
• Easy to Use: Add and incubate.
• Flexible: Use manually or on a robotic platform.
Storage Conditions: Store at 4–25°C.
Protocol Part#
FastBreak Cell Lysis Reagent Product Information 9PIV857
GST Protein Purification
MagneGST Protein Purification System
Product Size Cat.# Price ($)
MagneGST Protein Purification System 40 reactions V8600 245.00
Available Separately
200 reactions V8603 843.00
MagneGST Glutathione Particles 4 ml V8611 187.00
20 ml V8612 682.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneGST Protein Purification System provides a simple,
rapid and reliable method for the purification of glutathione-S-transferase (GST)
fusion proteins. Immobilized glutathione paramagnetic particles (MagneGST
Particles) are used to isolate GST-tagged protein directly from a crude or
cleared lysate using either a manual or automated procedure and requires use
of a magnetic stand. GST-tagged proteins can be purified on a small scale from
1ml of culture or on a large scale using more than 50ml of culture. Samples
also can be processed using a robotic platform.
Features:
• Simple: One-step purification of multiple samples with easy handling.
• Quick: After cell lysis, no requirement for high-speed centrifugation to
clear lysate.
• Scalable: Scalable protocol using 1–50ml of cell culture.
• Efficient: Achieve high yields with little or no nonspecific background.
• Complete: System contains all necessary components except magnetic
stand.
Storage Conditions: The complete system consists of two individual parts,
each with a different storage condition. Store individual boxes at specified
temperatures of 4°C and –70°C.
Protocol Part#
MagneGST Protein Purification System Technical Manual TM240
MagneGST Pull-Down System
Product Size Cat.# Price ($)
MagneGST Pull-Down System 80 reactions V8870 913.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The MagneGST Pull-Down System is designed for detection of
protein interactions between GST-fusion proteins expressed in bacterial lysates
and prey proteins expressed in the TnT ® Systems. Prey protein synthesized
in the TnT ® Quick Coupled Transcription/Translation Reaction is captured
using bait protein (GST-fusion protein) immobilized on MagneGST Particles.
Nonspecifically bound proteins are then washed away, and the prey protein is
analyzed. Prey proteins can be detected by incorporating radioactively labeled
methionine in the TnT ® Quick reaction, followed by SDS-PAGE and autoradiography
or by incorporating the supplied non-radioactive methionine in the TnT ®
reaction and detecting by Western blotting with protein-specific antibodies.
Storage Conditions: Store the TnT ® T7 Quick Master Mix and Methionine at
–70°C. Store the RQ1 RNase-Free DNase at –20°C. Store the Nuclease-Free
Water, MagneGST Glutathione Particles, MagneGST Binding/Wash Buffer
and Cell Lysis Reagent at 4°C.
Protocol Part#
MagneGST Pull-Down System Technical Manual TM249
Polyhistidine His-Tag Protein
Purification
Maxwell ® 16 Polyhistidine Protein Purification
Kit
Product Size Cat.# Price ($)
Maxwell ® 16 Polyhistidine Protein Purification
Kit 48 preps AS1060 Pls. Enq.
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Maxwell ® 16 Polyhistidine Protein Purification Kit is used
with the Maxwell ® 16 Instrument to provide an easy method for the efficient,
automated purification of polyhistidine-tagged protein from bacterial cultures
and other sample types including mammalian and insect cells. With minor
modifications, the reagents can also be used for purification of HQ-tagged
proteins from bacterial cultures.
The Maxwell ® Instrument is supplied with a preprogrammed purification
procedure and reagent cartridges specifically designed to maximize simplicity
and convenience. The instrument can process up to 16 samples in approximately
40 minutes. The purified protein is compatible with downstream applications
such as gel electrophoresis and Western blot analysis.
Features:
• Choose Your Sample Type: Flexibility to purify from multiple starting
cultures including bacterial culture, mammalian cells, insect cells and
culture medium.
• Have Confidence in Your Results: Achieve consistent purification across
all samples.
• Save Hands-On Time: Prefilled cartridges eliminate reagent preparation,
multiple pipetting steps, centrifugation and additional sample manipulation.
Storage Conditions: Store at 4°C.
Protocol Part#
Maxwell ® 16 Polyhistidine Protein Purification Kit Technical Manual TM285
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