2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 248 HaloLink Magnetic Beads Product Size Cat.# Price ($) HaloLink Magnetic Beads 40 reactions G9311 169.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: HaloLink Magnetic Beads provide a rapid and reliable method to covalently capture and immobilize HaloTag ® fusion proteins to a paramagnetic particle. Immobilization through the HaloTag ® Ligand provides for consistent, surface-directed orientation of the fusion protein, thus providing more reliable results in applications such as protein:protein interaction studies with the fusion protein. The result is a paramagnetic bead with very high binding capacity for HaloTag ® fusion proteins and very low nonspecific protein binding. The paramagnetic feature allows for streamlined assay development and facilitates applications with automated assays developed on robotic platforms. A variety of vectors for the expression of HaloTag ® fusion proteins in bacterial, mammalian or cell-free systems are available. Please see the HaloTag ® Technology Products page for more information. Detection of Protein: Protein Interactions HaloTag ® Fusion Protein B Add HaloLink Magnetic B Beads binding partner HaloTag ® Fusion Protein (bait) Overview of the HaloLink Magnetic Beads immobilization protocol. B B Bind (protein immobilization) Wash Remove nonspecific proteins Potential downstream applications Detection of Enzymatic Activity B Features: • Magnetic-Based: Easy and quick immobilization of HaloTag ® fusion proteins with minimal loss of sample material. • Covalent Bond: Allows stringent washing of immobilized HaloTag ® fusion protein, removing nonspecific proteins and enhancing overall data. • Multiple Applications: Immobilized HaloTag ® fusion proteins can be used for protein interaction analysis, enzyme assays and purification of fusion proteins containing a protease cleavage site. Storage Conditions: Store at 4°C. Protocol Part# HaloLink Magnetic Beads Technical Manual TM291 A B substrate product Purification of Nontagged Protein For complete and up-to-date product information visit: www.promega.com/catalog B Factor Xa cleavage 6630MA
Cell Signaling HaloCHIP System Product Size Cat.# Price ($) HaloCHIP System 20 reactions G9410 829.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloCHIP System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies and offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method. Proteins of interest are expressed in cells as HaloTag ® fusion proteins, crosslinked to DNA with formaldehyde and then captured on HaloLink Resin, which forms a highly specific, covalent interaction with the HaloTag ® portion of the fusion protein. Stringent washing removes nonspecific proteins and DNA, and heating reverses the crosslinks between the DNA and the fusion protein and releases the captured DNA fragment, which subsequently can be purified. HaloTag ® fusion construct Transfection HT TF HaloLink HaloLink Covalent Capture of Chromatin Complexes Using HaloTag ® Technology Experimental Sample HT HT TF TF Schematic diagram of the HaloCHIP System. For complete and up-to-date product information visit: www.promega.com/catalog TF HT TF HT HaloCHIP Blocking Ligand Controls Untransfected Cells OR Block HaloTag binding HaloLink HT Sample DNA Background DNA Analyze Analyze Features: • No Requirement for Antibody: No need to make your own or purchase expensive, qualified antibodies. • Obtain Results Faster: Obtain data in 24–48 hours with fewer steps to minimize potential experimental errors. • Improved Signal-to-Noise Ratios: Enables detection of small changes in protein binding patterns using a minimal number of cells. Storage Conditions: The TE Buffer (pH 8.0), Reversal Buffer and Nuclease- Free Water may be stored at room temperature. Store the HaloLink Resin, Mammalian Lysis Buffer and High Salt Wash Buffer at 4°C. Store the HaloCHIP Blocking Ligand at –20°C. Protocol Part# HaloCHIP System Technical Manual TM075 TF Expression of HaloTag ® Fusion Protein Formaldehyde Crosslinking Lysis, Sonication Split Sample Add HaloCHIP Blocking Ligand to the control sample to prevent binding to HaloLink Resin. Capture using the HaloLink Resin Wash HaloLink Resin Covalent capture allows highly stringent washes to remove nonspecific proteins and DNA. Release of DNA by reversal of crosslinks Protein remains bound to the HaloLink Resin. HT TF = HaloTag ® protein = Transcription factor 1–1.5 days 6633MB 249 14 Protein Purification and Interactions Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
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GloMax ® 20/20 Luminometer Product
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Fluorescence Module: Application-op
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GloMax ® -Multi Jr Single-Tube Mul
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Maxwell ® 16 Instruments Maxwell
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Maxwell ® 16 Flexi Method Firmware
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Page 201 and 202: Cell Signaling Maxwell ® 16 System
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Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
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Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
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Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251: Cell Signaling HaloLink Protein Arr
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291 and 292: Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294: Cell Signaling RNA Interference Gen
Page 295 and 296: Cell Signaling 17 Stem Cell Researc
Page 297 and 298: Cell Signaling Cell ID System Produ
Page 299 and 300: Cell Signaling StemElite Human Panc
Page 301 and 302: Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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