2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
246
HaloTEV Protease
Product Size Conc. Cat.# Price ($)
HaloTEV Protease 1,000 u 5 u/µl G6601 153.00
For Research Use Only. Not for Use in Diagnostic Procedures.
4,000 u 5 u/µl G6602 462.00
Description: HaloTEV Protease is a fusion between the HaloTag ® protein and
TEV protease, a highly specific proteolytic enzyme that cleaves at a specific TEV
site, a specific seven-amino-acid sequence (ENLYFQ(G/S)). HaloTEV Protease
allows for covalent immobilization on HaloLink Resin and removal from a
cleavage reaction in a single-step purification. The covalent capture of HaloTEV
Protease improves purity of the final target protein.
Features:
• Improve Final Protein Purity: Covalently remove HaloTEV from your
purified protein with HaloLink Resin.
• Optimized for HaloTag ® Purifications: Proteins can be purified tag-free
in a single step as the HaloLink Resin will bind both HaloTEV and the
HaloTag ® protein tag.
Storage Conditions: Store at –20°C.
HaloLink Resin
Product Size Cat.# Price ($)
HaloLink Resin 1.25 ml G1912 509.00
2.5 ml G1913 201.00
10 ml G1914 464.00
For Research Use Only. Not for Use in Diagnostic Procedures.
25 ml G1915 850.00
Description: The HaloLink Resin provides a method for covalent and
oriented attachment of HaloTag ® fusion proteins onto a solid surface. The resin
consists of a HaloTag ® ligand bound to Sepharose ® beads that specifically
and rapidly binds HaloTag ® fusion proteins. HaloLink Resin has high binding
capacity. Due to covalent linkage, HaloTag ® fusion proteins cannot be eluted
from the resin, allowing extensive washing to remove nonspecifically bound
protein without the danger of eluting HaloTag ® fusion proteins. The binding rate
is very rapid and equivalent to biotin-streptavidin.
The HaloLink Resin can be used in a variety of applications including:
detection and analysis of protein:protein interactions (in vivo and in vitro),
detection of enzymatic activity of immobilized HaloTag ® fusions and one-step
purification of fusion protein in conjunction with proteolytic cleavage. A variety
of vectors for the expression of HaloTag ® fusion proteins in bacterial, mammalian
or cell-free systems are available. Please see the HaloTag ® Technology
Products page for more information.
The HaloTag ® technology offers a quick and convenient way to test protein
expression of HaloTag ® fusion proteins as well as monitor the efficiency of
immobilization to HaloLink Resin by labeling with fluorescent HaloTag ® TMR
Ligand followed by SDS-PAGE analysis. Guidelines can be found in the Halo-
Link Resin Technical Manual #TM250.
Features:
• Covalent Attachment: Enables stringent washing, minimizing nonspecific
background without dissociation of bound HaloTag ® fusion proteins.
• Fast Binding Kinetics: Enhances the detection of protein:protein
interactions and enables binding of proteins at low concentrations.
• Oriented Immobilization: Allows maximal enzyme activity of bound
protein.
• High Binding Capacity: One milliliter of settled resin binds >7mg of
HaloTag ® fusion proteins.
Storage Conditions: Store at 4°C.
Protocol Part#
HaloLink Resin Technical Manual TM250
A. B.
c-jun
HaloTag ® -fusion
(c-fos)
HaloTag ® ligand
Surface
c-jun
HaloTag ® -fusion
(c-fos)
HaloTag ® ligand
Surface
Detection of protein:protein interactions using the HaloLink Resin.
Panel A. HaloTag ® fusion protein (bait, HaloTag ® c-fos) is expressed in TnT ®
T7 Quick Coupled Transcription/Translation System and immobilized to the
HaloLink Resin. The partner (prey) c-jun is expressed in TnT ® reactions and
mixed with the immobilized HaloTag ® c-fos and allowed to bind. Panel B. Both
interacting partners, bait and prey HaloTag ® fusions are expressed in individual
TnT ® reactions, mixed and allowed to bind; then the HaloLink Resin is
added, and the complex is captured.
For complete and up-to-date product information visit: www.promega.com/catalog
5308TB