2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling ADME Assays 20 CYP450 Assay Systems Product Size Cat.# Price ($) P450-Glo CYP1A2 Induction/Inhibition Assay 10 ml V8421 181.00 50 ml V8422 523.00 P450-Glo CYP3A4 Assay with Luciferin-IPA 10 ml V9001 181.00 50 ml V9002 523.00 P450-Glo CYP3A4 Assay (Luciferin-PPXE) 10 ml V8911 181.00 DMSO-Tolerant Assay 50 ml V8912 523.00 P450-Glo CYP3A4 Assay (Luciferin-PFBE) 10 ml V8901 181.00 Cell-Based/Biochemical Assay 50 ml V8902 523.00 P450-Glo CYP1A1 Assay 10 ml V8751 181.00 50 ml V8752 523.00 P450-Glo CYP1B1 Assay 10 ml V8761 181.00 50 ml V8762 523.00 P450-Glo CYP1A2 Assay 10 ml V8771 181.00 50 ml V8772 523.00 P450-Glo CYP2C8 Assay 10 ml V8781 181.00 50 ml V8782 523.00 P450-Glo CYP2C9 Assay 10 ml V8791 181.00 50 ml V8792 523.00 P450-Glo CYP3A4 Assay 10 ml V8801 181.00 50 ml V8802 523.00 P450-Glo CYP3A7 Assay 10 ml V8811 181.00 50 ml V8812 523.00 P450-Glo CYP2C19 Assay 10 ml V8881 181.00 50 ml V8882 523.00 P450-Glo CYP2D6 Assay 10 ml V8891 181.00 Available Separately 50 ml V8892 523.00 NADPH Regeneration System 1,000 assays V9510 180.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The P450-Glo CYP450 Assays provide a homogeneous, luminescent method for measuring cytochrome P450 activity. The assays are designed to measure the activities of P450s from recombinant and native sources and for testing the effects of analytes such as drugs and new chemical entities on P450 activities. These luminescent assays exhibit exquisite sensitivity, low background signals and broad dynamic range. P450-Glo Assays employ luminogenic P450 substrates that are derivatives of beetle luciferin. The derivatives are not substrates for luciferase but are converted by P450s to luciferin, which in turn reacts with luciferase to produce light that is directly proportional to the activity of the P450. The P450-Glo Assays generate a “glow-type” luminescent signal, produced using a recombinant stabilized luciferase (Ultra-Glo Luciferase) coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing. The formulation also minimizes the incidence of false positives due to inhibition of luciferase by analytes when screening for cytochrome P450 inhibitors. The new P450-Glo CYP3A4 Assay with Luciferin-IPA contains a new substrate for cytochrome 3A4 that is very well suited for all applications involving human CYP3A4 and is the best substrate available for cell-based applications. Luciferin-IPA is readily taken up by cells and rapidly converted into luciferin inside the cell, which reduces the incubation time required. The low background and high signal-to-noise ratio produced using Luciferin-IPA means less starting material is required. Dimethyl sulfoxide (DMSO) can inhibit the activity of the 3A4 isoform of cytochrome P450, even at low concentrations (
Cell Signaling A. B. Luminescence/pmol CYP450 Luminescence/pmol CYP450 Luminescence/pmol CYP450 Luminescence/pmol CYP450 175,000 150,000 125,000 100,000 75,000 50,000 25,000 0 Luciferin-CEE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 For complete and up-to-date product information visit: www.promega.com/catalog Luminescence/pmol CYP450 C. D. E. G. 1,300,000 200,000 150,000 100,000 50,000 0 650,000 550,000 450,000 350,000 250,000 150,000 50,000 450,000 100,000 75,000 50,000 25,000 0 Luciferin-BE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Luciferin-H EGE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Luciferin-PFBE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Selectivity of the P450-Glo substrates for human CYP450 enzymes. Luminescence/pmol CYP450 F. Luminescence/pmol CYP450 H. Luminescence/pmol CYP450 225,000 125,000 100,000 75,000 50,000 25,000 0 400,000 300,000 200,000 100,000 0 1,500,000 500,000 375,000 250,000 125,000 400,000 300,000 200,000 100,000 0 0 Luciferin-ME control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Luciferin-H control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Luciferin-ME EGE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 Luciferin-PPXE control CYP1A1 CYP1A2 CYP1B1 CYP2A6 CYP2B6 CYP2C8 CYP2C9 CYP2C18 CYP2C19 CYP2D6 CYP2E1 CYP2J2 CYP3A4 CYP3A5 CYP3A7 CYP4A11 CYP4F2 CYP4F3A CYP4F3B CYP4F12 CYP19 5563MC 21 2 Cell Health Assays Section Contents Table of Contents
Page 1 and 2: Life Science CATALOG 2012
Page 3 and 4: Cell Signaling Table of Contents Pr
Page 5 and 6: Cell Signaling For complete and up-
Page 7 and 8: Cell Signaling Agarose, LMP, Prepar
Page 9 and 10: Cell Signaling Blue/Orange Loading
Page 11 and 12: Cell Signaling Glycine, Molecular B
Page 13 and 14: Cell Signaling Sephacryl ® S-400 P
Page 15 and 16: Cell Signaling Tris Base, Molecular
Page 17 and 18: Cell Signaling Unmethylated Lambda
Page 19 and 20: Cell Signaling Promega Barrier Tips
Page 21 and 22: Cell Signaling Product Size Cat.# P
Page 23: Cell Signaling 2 Cell Health Assays
Page 27 and 28: Cell Signaling Luminogenic Enzyme S
Page 29 and 30: Cell Signaling Apoptosis
Page 31 and 32: Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34: Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36: Cell Signaling CaspACE Assay System
Page 37 and 38: Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40: Cell Signaling BacTiter-Glo Microbi
Page 41 and 42: Cell Signaling CellTiter 96 ® Cell
Page 43 and 44: Cell Signaling MultiTox-Fluor Multi
Page 45 and 46: Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48: Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50: Cell Signaling GSH-Glo Glutathione
Page 51 and 52: Cell Signaling 3 Cell Signaling For
Page 53 and 54: Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56: Cell Signaling Growth Factors Epide
Page 57 and 58: Cell Signaling SIRT-Glo Assays and
Page 59 and 60: Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62: Cell Signaling Kinase Enzyme System
Page 63 and 64: Cell Signaling Kinase Enzyme System
Page 65 and 66: Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68: Cell Signaling SignaTECT ® Protein
Page 69 and 70: Cell Signaling Casein Kinase I Prod
Page 71 and 72: Cell Signaling Product Size Cat.# P
Page 73 and 74: Cell Signaling Protein Kinase Subst
Page 75 and 76:
Cell Signaling For complete and up-
Page 77 and 78:
Cell Signaling DNA Step Ladders Pro
Page 79 and 80:
Cell Signaling Conventional DNA Mar
Page 81 and 82:
Cell Signaling ProMega-Markers ® L
Page 83 and 84:
Cell Signaling Restriction Enzymes
Page 85 and 86:
Cell Signaling BamHI Product Size C
Page 87 and 88:
Cell Signaling EcoRI Product Size C
Page 89 and 90:
Cell Signaling NciI Product Size Co
Page 91 and 92:
Cell Signaling SpeI Product Size Co
Page 93 and 94:
Cell Signaling TSAP Thermosensitive
Page 95 and 96:
Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98:
Cell Signaling Kinases T4 Polynucle
Page 99 and 100:
Cell Signaling S1 Nuclease Product
Page 101 and 102:
Cell Signaling Percent Inhibition o
Page 103 and 104:
Cell Signaling Untagged Flexi ® Ma
Page 105 and 106:
Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108:
Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110:
Page 111 and 112:
Cell Signaling x-tracta Gel Extract
Page 113 and 114:
Cell Signaling Wizard ® MagneSil
Page 115 and 116:
Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118:
Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120:
Cell Signaling MagneSil ® KF, Geno
Page 121 and 122:
Cell Signaling Plasmid Purification
Page 123 and 124:
Cell Signaling PureYield Plasmid Ma
Page 125 and 126:
Cell Signaling Wizard ® Plus Maxip
Page 127 and 128:
Cell Signaling Wizard ® MagneSil
Page 129 and 130:
Cell Signaling PureYield RNA Midipr
Page 131 and 132:
Cell Signaling Maxwell ® 16 System
Page 133 and 134:
Cell Signaling RNasin ® Plus RNase
Page 135 and 136:
Cell Signaling DNA Quantitation Qua
Page 137 and 138:
Cell Signaling Magnetic Stands and
Page 139 and 140:
Cell Signaling 6 Drug Discovery For
Page 141 and 142:
Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144:
Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146:
Cell Signaling Protease Assays Prot
Page 147 and 148:
Cell Signaling Cell-Based Proteasom
Page 149 and 150:
Cell Signaling Tryptase, Human, Rec
Page 151 and 152:
Cell Signaling 7 Genetic Identity F
Page 153 and 154:
Cell Signaling Maxwell ® 16 Forens
Page 155 and 156:
Cell Signaling Plexor ® HY System
Page 157 and 158:
Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160:
Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162:
Cell Signaling PowerPlex ® 16 and
Page 163 and 164:
Cell Signaling GenePrint ® Fluores
Page 165 and 166:
Page 167 and 168:
Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170:
Cell Signaling Block & Sample 5X Bu
Page 171 and 172:
Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174:
Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176:
Cell Signaling Anti-Human GDNF pAb
Page 177 and 178:
Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180:
Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182:
Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184:
Page 185 and 186:
Cell Signaling Microbial Detection
Page 187 and 188:
10 Instruments For complete and up-
Page 189 and 190:
GloMax ® 20/20 Luminometer Product
Page 191 and 192:
Fluorescence Module: Application-op
Page 193 and 194:
GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196:
Maxwell ® 16 Instruments Maxwell
Page 197 and 198:
Maxwell ® 16 Flexi Method Firmware
Page 199 and 200:
Page 201 and 202:
Cell Signaling Maxwell ® 16 System
Page 203 and 204:
Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206:
Cell Signaling dNTP Mix Product Siz
Page 207 and 208:
Cell Signaling 12 PCR For complete
Page 209 and 210:
Cell Signaling TaqBead Hot Start Po
Page 211 and 212:
Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214:
Cell Signaling Deoxynucleotide Trip
Page 215 and 216:
Cell Signaling Plexor ® qPCR and q
Page 217 and 218:
Cell Signaling StemElite Differenti
Page 219 and 220:
Cell Signaling Reverse Transcriptio
Page 221 and 222:
Cell Signaling AMV Reverse Transcri
Page 223 and 224:
Cell Signaling PCR Cloning pGEM ®
Page 225 and 226:
Page 227 and 228:
Cell Signaling TnT ® Quick Coupled
Page 229 and 230:
Cell Signaling TnT ® T7 Quick for
Page 231 and 232:
Cell Signaling Canine Pancreatic Mi
Page 233 and 234:
Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236:
Cell Signaling Immobilized Trypsin
Page 237 and 238:
Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240:
Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242:
Cell Signaling Inducible T7-Driven
Page 243 and 244:
Cell Signaling ProtoBlot ® II AP S
Page 245 and 246:
Page 247 and 248:
Cell Signaling HaloTag ® Protein P
Page 249 and 250:
Cell Signaling HaloTag ® Mammalian
Page 251 and 252:
Cell Signaling HaloLink Protein Arr
Page 253 and 254:
Cell Signaling HaloCHIP System Prod
Page 255 and 256:
Cell Signaling MagneHis Protein Pur
Page 257 and 258:
Cell Signaling Streptavidin Product
Page 259 and 260:
Cell Signaling Protein Purification
Page 261 and 262:
Cell Signaling 15 Reporter Assays a
Page 263 and 264:
Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266:
Cell Signaling Firefly Luciferase R
Page 267 and 268:
Cell Signaling Bright-Glo Luciferas
Page 269 and 270:
Cell Signaling EnduRen Live Cell Su
Page 271 and 272:
Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274:
Cell Signaling Promoter-Driven Cont
Page 275 and 276:
Cell Signaling Nuclear Receptor Pat
Page 277 and 278:
Cell Signaling pRL Renilla Lucifera
Page 279 and 280:
Cell Signaling GloResponse Lucifera
Page 281 and 282:
Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284:
Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286:
Cell Signaling Transfection Reagent
Page 287 and 288:
Cell Signaling 16 RNA Analysis For
Page 289 and 290:
Cell Signaling Riboprobe ® System
Page 291 and 292:
Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294:
Cell Signaling RNA Interference Gen
Page 295 and 296:
Cell Signaling 17 Stem Cell Researc
Page 297 and 298:
Cell Signaling Cell ID System Produ
Page 299 and 300:
Cell Signaling StemElite Human Panc
Page 301 and 302:
Cell Signaling 18 Vectors For compl
Page 303 and 304:
Cell Signaling HaloTag ® Fusion (N
Page 305 and 306:
Cell Signaling pCI Mammalian Expres
Page 307 and 308:
Cell Signaling Reporter Vectors pGL
Page 309 and 310:
Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312:
Cell Signaling pGEM ® and pSP Subc
Page 313 and 314:
Cell Signaling pUC/M13 Sequencing P
Page 315 and 316:
Index: A-Z 19 Index For complete an
Page 317 and 318:
BssHII.............................
Page 319 and 320:
In vitro Transcription Systems Rela
Page 321 and 322:
QuantiFluor dsDNA System ..........
Page 323 and 324:
Cat# Product Size Page Price($) A10
Page 325 and 326:
Index by Catalog Number Cat# Produc
Page 327 and 328:
Cat# Product Size Page Price($) C84
Page 329 and 330:
Cat# Product Size Page Price($) E13
Page 331 and 332:
Cat# Product Size Page Price($) E61
Page 333 and 334:
Cat# Product Size Page Price($) G13
Page 335 and 336:
Cat# Product Size Page Price($) G77
Page 337 and 338:
Cat# Product Size Page Price($) L50
Page 339 and 340:
Cat# Product Size Page Price($) P16
Page 341 and 342:
Cat# Product Size Page Price($) R99
Page 343 and 344:
Cat# Product Size Page Price($) V42
Page 345 and 346:
Cat# Product Size Page Price($) W39
Page 347 and 348:
personal DETECTION GloMax Integrat
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2012 Promega catalogue

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