2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
244
HaloTag ® Mammalian Protein Purification
System
Product Size Cat.# Price ($)
HaloTag ® Mammalian Protein Detection and
Purification System 1 each G6795 953.00
HaloTag ® Mammalian Protein Purification System 1 each G6790 788.00
HaloTag ® Mammalian Protein Detection and
Purification System Sample Pack 1 each G6799 392.00
Available Separately
HaloTEV Protease 1,000 u 5 u/µl G6601 153.00
4,000 u 5 u/µl G6602 462.00
HaloTag ® TMRDirect Ligand 30 µl 0.1 mM G2991 521.00
Protease Inhibitor Cocktail, 50X 1 ml G6521 87.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The HaloTag ® Mammalian Protein Purification System
(Cat.# G6790) is an optimized kit for purification of HaloTag ® fusion proteins
from mammalian cell culture lysates. HaloTag ® fusion proteins form a highly
specific and covalent bond with the HaloLink Resin. The covalent binding
coupled with the low nonspecific binding of the HaloLink Resin provides
superior purity and recovery of recombinant proteins from cultured mammalian
cells, even at low expression levels. The HaloTag ® Mammalian Protein
Detection and Purification System (Cat.# G6795) also includes HaloTag ®
TMRDirect Ligand. The simple-to-use fluorescent detection of the HaloTag ®
fusion facilitates rapid optimization of expression and purification conditions.
Schematic of the HaloTag ® Mammalian Protein Purification System
protocol. Streamlined purification process leads to higher purity and
recovery of recombinant proteins from cultured cells.
Features:
• Purify More Protein: HaloLink Resin covalently binds >7mg/ml of
HaloTag ® fusion protein (10X more capacity compared to FLAG ® ).
Recovery is highly efficient, commonly >75%.
• Higher Purity: Covalent capture allows extensive and/or stringent washes
without loss of bound protein, resulting in very low (