2012 Promega catalogue

More documents

Recommendations

Info

The robust functionality allows you to… • Search for genes by blasting DNA, RNA, or amino acid sequences. • Find detailed information about your gene of interest. • Explore literature-validated protein interactions. • Find relevant products targeted specifically to a gene of interest. Get Interactive with Your Gene Search find my gene Search by ORF Clone Name, Gene Symbol/ID/Name, Accession #, Alternate Search by Protein or Nucleic Acid Sequence Learn more about your gene at: www.promega.com/FMG ©<strong>2012</strong>, <strong>Promega</strong> Corporation. All Rights Reserved.
Cell Signaling HaloTag ® Protein Purification HaloTag ® Protein Purification System Product Size Cat.# Price ($) HaloTag ® Protein Purification System 1 each G6280 907.00 HaloTag ® Protein Purification System Sample Pack 1 each G6270 306.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The HaloTag ® Protein Purification System is designed to purify proteins fused to HaloTag ® novel protein tag that enhances the expression and solubility of recombinant proteins. HaloTag ® Technology enables the covalent, efficient and specific capture of a protein of interest onto HaloLink Resin, thus overcoming the equilibrium-based limitations associated with affinity tags (i.e., poor capture of proteins expressed at low levels and protein loss during washing of the purification resin. The HaloTag ® technology offers a quick and convenient way to test protein expression of HaloTag ® fusion proteins as well as monitor the efficiency of immobilization to HaloLink Resin by labeling with fluorescent HaloTag ® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the Halo- Link Resin Technical Manual #TM250, the HaloLink Protein Array Technical Manual #TM310, and the HaloCHIP System Technical Manual #TM075. Outline of Procedure The HaloTag ® protein, a 34kDa mutated hydrolase, covalently attaches to HaloLink Resin via an immobilized chloroalkane ligand. TEV Protease cleaves the target protein from the HaloLink Resin. The TEV Protease, which has an N-terminal (HQ) tag, is removed from the protein of interest using HisLink Resin, and the purified protein of interest is recovered. The appropriate vector that encodes the HaloTag ® protein and expresses protein optimally in E. coli is pFN18A HaloTag ® T7 Flexi ® Vector (G2751) or pFN18K HaloTag ® T7 Flexi ® Vector (G2681). These vectors can be purchased separately. For complete and up-to-date product information visit: www.promega.com/catalog Features: • Experience Superior Yield, Purity and Specific Activity of Soluble, Functional Proteins Compared to His-Tag, GST and MBP Affinity Tags: Specific and covalent HaloTag ® fusion protein capture and immobilization on HaloLink Resin. • Achieve Enhanced Target Protein Expression in Prokaryotic, Mammalian and Cell-Free Systems: Proteins are expressed as HaloTag ® fusion proteins. • Purify Poorly Expressed Fusion Proteins: Rapid, specific and covalent capture of HaloTag ® protein onto HaloLink Resin is a nonequilibrium process. • Efficiently Recover Tag-Free Target Protein using TEV Protease Cleavage: Optimized TEV protease recognition site within the interconnecting polypeptide separating the HaloTag ® protein and the fusion partner. HaloTag ® protein remains immobilized on the resin due to covalent capture. • Save Time: One buffer compatible with downstream applications for all purification steps. • Perform Easy In-Gel Detection and Quantification of Protein Expression Levels with Fluorescent HaloTag ® Ligands: Highly stable HaloTag ® protein-ligand interaction permits boiling with SDS sample buffer followed by resolving on SDS-PAGE. Storage Conditions: Store the HaloLink Resin and HisLink Resin at 4°C. Do not freeze the resins. Store the TEV Protease at –20°C. Protocol Part# HaloTag ® Protein Purification System Technical Manual TM312 243 14 Protein Purification and Interactions Section Contents Table of Contents
Page 1 and 2:
Life Science CATALOG 2012
Page 3 and 4:
Cell Signaling Table of Contents Pr
Page 5 and 6:
Cell Signaling For complete and up-
Page 7 and 8:
Cell Signaling Agarose, LMP, Prepar
Page 9 and 10:
Cell Signaling Blue/Orange Loading
Page 11 and 12:
Cell Signaling Glycine, Molecular B
Page 13 and 14:
Cell Signaling Sephacryl ® S-400 P
Page 15 and 16:
Cell Signaling Tris Base, Molecular
Page 17 and 18:
Cell Signaling Unmethylated Lambda
Page 19 and 20:
Cell Signaling Promega Barrier Tips
Page 21 and 22:
Cell Signaling Product Size Cat.# P
Page 23 and 24:
Cell Signaling 2 Cell Health Assays
Page 25 and 26:
Cell Signaling A. B. Luminescence/p
Page 27 and 28:
Cell Signaling Luminogenic Enzyme S
Page 29 and 30:
Cell Signaling Apoptosis
Page 31 and 32:
Cell Signaling Caspase-Glo ® 2 Ass
Page 33 and 34:
Cell Signaling Caspase-Glo ® 9 Ass
Page 35 and 36:
Cell Signaling CaspACE Assay System
Page 37 and 38:
Cell Signaling Anti-ACTIVE ® Caspa
Page 39 and 40:
Cell Signaling BacTiter-Glo Microbi
Page 41 and 42:
Cell Signaling CellTiter 96 ® Cell
Page 43 and 44:
Cell Signaling MultiTox-Fluor Multi
Page 45 and 46:
Cell Signaling CytoTox-Glo Cytotoxi
Page 47 and 48:
Cell Signaling CytoTox 96 ® Non-Ra
Page 49 and 50:
Cell Signaling GSH-Glo Glutathione
Page 51 and 52:
Cell Signaling 3 Cell Signaling For
Page 53 and 54:
Cell Signaling cAMP-Glo Max Assay P
Page 55 and 56:
Cell Signaling Growth Factors Epide
Page 57 and 58:
Cell Signaling SIRT-Glo Assays and
Page 59 and 60:
Cell Signaling ADP-Glo Max Assay Pr
Page 61 and 62:
Cell Signaling Kinase Enzyme System
Page 63 and 64:
Cell Signaling Kinase Enzyme System
Page 65 and 66:
Cell Signaling Kinase-Glo ® Kinase
Page 67 and 68:
Cell Signaling SignaTECT ® Protein
Page 69 and 70:
Cell Signaling Casein Kinase I Prod
Page 71 and 72:
Cell Signaling Product Size Cat.# P
Page 73 and 74:
Cell Signaling Protein Kinase Subst
Page 75 and 76:
Page 77 and 78:
Cell Signaling DNA Step Ladders Pro
Page 79 and 80:
Cell Signaling Conventional DNA Mar
Page 81 and 82:
Cell Signaling ProMega-Markers ® L
Page 83 and 84:
Cell Signaling Restriction Enzymes
Page 85 and 86:
Cell Signaling BamHI Product Size C
Page 87 and 88:
Cell Signaling EcoRI Product Size C
Page 89 and 90:
Cell Signaling NciI Product Size Co
Page 91 and 92:
Cell Signaling SpeI Product Size Co
Page 93 and 94:
Cell Signaling TSAP Thermosensitive
Page 95 and 96:
Cell Signaling T3 RNA Polymerase Pr
Page 97 and 98:
Cell Signaling Kinases T4 Polynucle
Page 99 and 100:
Cell Signaling S1 Nuclease Product
Page 101 and 102:
Cell Signaling Percent Inhibition o
Page 103 and 104:
Cell Signaling Untagged Flexi ® Ma
Page 105 and 106:
Cell Signaling pGEM ® -5Zf(+) Vect
Page 107 and 108:
Cell Signaling pSP64 Poly(A) Vector
Page 109 and 110:
Page 111 and 112:
Cell Signaling x-tracta Gel Extract
Page 113 and 114:
Cell Signaling Wizard ® MagneSil
Page 115 and 116:
Cell Signaling ReliaPrep FFPE gDNA
Page 117 and 118:
Cell Signaling MagneSil ® ONE, Fix
Page 119 and 120:
Cell Signaling MagneSil ® KF, Geno
Page 121 and 122:
Cell Signaling Plasmid Purification
Page 123 and 124:
Cell Signaling PureYield Plasmid Ma
Page 125 and 126:
Cell Signaling Wizard ® Plus Maxip
Page 127 and 128:
Cell Signaling Wizard ® MagneSil
Page 129 and 130:
Cell Signaling PureYield RNA Midipr
Page 131 and 132:
Cell Signaling Maxwell ® 16 System
Page 133 and 134:
Cell Signaling RNasin ® Plus RNase
Page 135 and 136:
Cell Signaling DNA Quantitation Qua
Page 137 and 138:
Cell Signaling Magnetic Stands and
Page 139 and 140:
Cell Signaling 6 Drug Discovery For
Page 141 and 142:
Cell Signaling GPCR Assays cAMP-Glo
Page 143 and 144:
Cell Signaling PDE-Glo Phosphodiest
Page 145 and 146:
Cell Signaling Protease Assays Prot
Page 147 and 148:
Cell Signaling Cell-Based Proteasom
Page 149 and 150:
Cell Signaling Tryptase, Human, Rec
Page 151 and 152:
Cell Signaling 7 Genetic Identity F
Page 153 and 154:
Cell Signaling Maxwell ® 16 Forens
Page 155 and 156:
Cell Signaling Plexor ® HY System
Page 157 and 158:
Cell Signaling PowerPlex ® 18D Sys
Page 159 and 160:
Cell Signaling PowerPlex ® CS7 Sys
Page 161 and 162:
Cell Signaling PowerPlex ® 16 and
Page 163 and 164:
Cell Signaling GenePrint ® Fluores
Page 165 and 166:
Page 167 and 168:
Cell Signaling HaloTag ® Ligand Bu
Page 169 and 170:
Cell Signaling Block & Sample 5X Bu
Page 171 and 172:
Cell Signaling Anti-ACTIVE ® JNK p
Page 173 and 174:
Cell Signaling Anti-ERK 1/2 pAb, Ra
Page 175 and 176:
Cell Signaling Anti-Human GDNF pAb
Page 177 and 178:
Cell Signaling Anti-PARP p85 Fragme
Page 179 and 180:
Cell Signaling Anti-VAChT pAb Produ
Page 181 and 182:
Cell Signaling VivoGlo Caspase 3/7
Page 183 and 184:
Page 185 and 186:
Cell Signaling Microbial Detection
Page 187 and 188:
10 Instruments For complete and up-
Page 189 and 190:
GloMax ® 20/20 Luminometer Product
Page 191 and 192:
Fluorescence Module: Application-op
Page 193 and 194:
GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
Page 199 and 200: Cell Signaling For complete and up-
Page 201 and 202: Cell Signaling Maxwell ® 16 System
Page 203 and 204: Cell Signaling Maxwell ® 16 Flexi
Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 225 and 226: Cell Signaling For complete and up-
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239 and 240: Cell Signaling HaloTag ® Ligand Bu
Page 241 and 242: Cell Signaling Inducible T7-Driven
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 245: Cell Signaling For complete and up-
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
Page 291 and 292: Cell Signaling HeLaScribe ® Nuclea
Page 293 and 294: Cell Signaling RNA Interference Gen
Page 295 and 296: Cell Signaling 17 Stem Cell Researc
Page 297 and 298:
Cell Signaling Cell ID System Produ
Page 299 and 300:
Cell Signaling StemElite Human Panc
Page 301 and 302:
Cell Signaling 18 Vectors For compl
Page 303 and 304:
Cell Signaling HaloTag ® Fusion (N
Page 305 and 306:
Cell Signaling pCI Mammalian Expres
Page 307 and 308:
Cell Signaling Reporter Vectors pGL
Page 309 and 310:
Cell Signaling pGEM ® -luc DNA Pro
Page 311 and 312:
Cell Signaling pGEM ® and pSP Subc
Page 313 and 314:
Cell Signaling pUC/M13 Sequencing P
Page 315 and 316:
Index: A-Z 19 Index For complete an
Page 317 and 318:
BssHII.............................
Page 319 and 320:
In vitro Transcription Systems Rela
Page 321 and 322:
QuantiFluor dsDNA System ..........
Page 323 and 324:
Cat# Product Size Page Price($) A10
Page 325 and 326:
Index by Catalog Number Cat# Produc
Page 327 and 328:
Cat# Product Size Page Price($) C84
Page 329 and 330:
Cat# Product Size Page Price($) E13
Page 331 and 332:
Cat# Product Size Page Price($) E61
Page 333 and 334:
Cat# Product Size Page Price($) G13
Page 335 and 336:
Cat# Product Size Page Price($) G77
Page 337 and 338:
Cat# Product Size Page Price($) L50
Page 339 and 340:
Cat# Product Size Page Price($) P16
Page 341 and 342:
Cat# Product Size Page Price($) R99
Page 343 and 344:
Cat# Product Size Page Price($) V42
Page 345 and 346:
Cat# Product Size Page Price($) W39
Page 347 and 348:
personal DETECTION GloMax Integrat
show all

2012 Promega catalogue

Create successful ePaper yourself

Delete template?

Save as template?