2012 Promega catalogue

Cell Signaling
ProtoBlot ® II AP Systems with Stabilized
Substrate and Western Express ® Fast Blotting
Protocol
Product Size Cat.# Price ($)
ProtoBlot ® II AP System with Stabilized Substrate,
Human 1 each W3940 399.00
ProtoBlot ® II AP System with Stabilized Substrate,
Mouse 1 each W3950 399.00
ProtoBlot ® II AP System with Stabilized Substrate,
Rabbit 1 each W3960 399.00
For Laboratory Use.
Description: The ProtoBlot ® II AP Systems with Stabilized Substrate are
designed for the rapid and sensitive detection of proteins or other macromolecular
antigens immobilized on nitrocellulose or PVDF membranes. Proteins
can be transferred from gels after electrophoresis (Western blots) or bound
directly from solution (“dot” blots). The Western Express ® Fast Blotting Protocol
is included with the system and can reduce dramatically the time required
to perform immunodetection. All ProtoBlot ® II AP Systems contain BSA as a
stabilizer and 0.05% sodium azide as a preservative.
Features:
• Fast: Easy-to-use Western Express ® Protocol allows the detection of dot
blots in 30–45 minutes and the detection of Western blots in 1–2 hours.
• Convenient: The system contains Western Blue ® Stabilized Substrate for
AP, which is a ready-to-use solution of BCIP/NBT. No reagent preparation is
required for the substrate.
For many applications, AP conjugates are superior to HRP conjugates
because they:
• offer greater sensitivity (tenfold) of detection.
• are not inhibited by azide.
• use a substrate that is not prone to fading during long-term storage.
• have protocols provided for both PVDF and nitrocellulose membranes.
Storage Conditions: Store antibody conjugates at 4°C (undiluted). Store
Western Blue ® Substrate at room temperature.
Protocol Part#
ProtoBlot ® II AP System with Stabilized Substrate Technical Manual
Western Blue ® Stabilized Substrate for
Alkaline Phosphatase
TM026
Product Size Cat.# Price ($)
Western Blue ® Stabilized Substrate for Alkaline
Phosphatase 100 ml S3841 87.00
For Research Use Only. Not for Use in Diagnostic Procedures.
For additional information see page 12.
TMB Stabilized Substrate for Horseradish
Peroxidase
Product Size Cat.# Price ($)
TMB Stabilized Substrate for Horseradish
Peroxidase
For Research Use Only. Not for Use in Diagnostic Procedures.
200 ml W4121 282.00
For additional information see page 10.
For complete and up-to-date product information visit: www.promega.com/catalog
BCIP/NBT Color Development Substrate
(5-bromo-4-chloro-3-indolyl-phosphate/nitro
blue tetrazolium)
Product Size Cat.# Price ($)
BCIP/NBT Color Development Substrate
For Laboratory Use.
1.25/2.5 ml S3771 123.00
For additional information see page 4.
239
13
Protein Expression and Analysis
Section
Contents
Table of
Contents