2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
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Table of
Contents
Cell Signaling
238
Transcend Non-Radioactive Translation
Detection Systems
Product Size Cat.# Price ($)
Transcend Colorimetric Translation
Detection System
Transcend Chemiluminescent Translation
30 reactions L5070 377.00
Detection System
Available Separately
30 reactions L5080 566.00
Transcend tRNA 30 µl L5061 307.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The Transcend Non-Radioactive Translation Detection
Systems allow non-radioactive detection of proteins synthesized in vitro.
Using these systems, biotinylated lysine residues are incorporated into
nascent proteins during translation, eliminating the need for labeling with [ 35 S]
methionine or other radioactive amino acids. This biotinylated lysine is added
to the translation reaction as a precharged ε-labeled biotinylated lysine-tRNA
complex (Transcend tRNA) rather than a free amino acid. After SDS-PAGE
and electroblotting, the biotinylated proteins can be visualized by binding either
Streptavidin-Alkaline Phosphatase (Streptavidin-AP) or Streptavidin-Horseradish
Peroxidase (Streptavidin-HRP), followed either by colorimetric or chemiluminescent
detection. Typically, these methods can detect 0.5–5ng of protein
within 3–4 hours after gel electrophoresis. This sensitivity is equivalent to that
achieved with [ 35 S]methionine incorporation and autoradiographic detection
6–12 hours after gel electrophoresis.
Features:
• Sensitive: The biotin tag allows detection of 0.5–5ng of translated protein.
• Safe: No radioisotope handling, storage or disposal is required.
• Fast: Labeled proteins can be detected 3–4 hours after gel electrophoresis.
• Flexible: Results can be visualized by using colorimetric or chemiluminescent
detection.
Storage Conditions: Store Transcend tRNA at –70°C. Do not subject
the Transcend tRNA to more than five freeze-thaw cycles. Store all other
components at 4°C.
Protocol Part#
Transcend Non-Radioactive Translation Detection System
Technical Bulletin
TB182
ECL Western Blotting Substrate
Product Size Cat.# Price ($)
ECL Western Blotting Substrate 250 ml W1001 247.00
For Research Use Only. Not for Use in Diagnostic Procedures.
500 ml W1015 387.00
Description: The ECL Western Blotting Substrate is a highly sensitive
non-radioactive, enhanced luminol-based chemiluminescent substrate for the
detection of horseradish peroxidase (HRP) on immunoblots. The ECL Western
Blotting Substrate detects picogram amounts of antigen, and with the use of
photographic or other imaging methods, visualizes the presence of HRP.
Features:
• High Sensitivity: Detect picogram levels of protein.
• Save Time: No optimization required; you can switch from other entrylevel
ECL substrates.
Storage Conditions: Store at 2–8°C.
Protocol Part#
ECL Western Blotting Substrate Technical Manual TM317
TMB One Solution
Product Size Cat.# Price ($)
TMB One Solution
For Laboratory Use.
100 ml G7431 144.00
For additional information see page 176.
AttoPhos ® AP Fluorescent Substrate System
Product Size Cat.# Price ($)
AttoPhos ® AP Fluorescent Substrate System
AttoPhos
3 × 36 mg S1000 727.00
® AP Fluorescent Substrate System
Trial Size
Available Separately
1 × 36 mg S1001 295.00
AttoPhos ® Substrate 36 mg S1011 192.00
100 mg S1012 474.00
1 g S1013 1884.00
AttoPhos ® Buffer 60 ml S1021 114.00
240 ml S1022 349.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: AttoPhos ® AP Fluorescent Substrate System contains a highly
sensitive fluorescent alkaline phosphatase (AP) substrate.
Features:
• Sensitivity: Low fluorescence signal until enzymatically acted upon, yielding
detection of AP to 0.1 attomole.
• Low Background: Low fluorescence from interfering biological molecules.
• Linearity: Linear kinetics over five orders of magnitude of AP concentration.
• Additional Features: Excitation at 435nm, emission at 555nm and large
Stokes’ shift (≈120nm).
Storage Conditions: Store at 4°C.
Protocol Part#
AttoPhos ® AP Fluorescent Substrate System Technical Bulletin TB280
Blocking Agents
Product Size Cat.# Price ($)
Blot-Qualified BSA 10 g W3841 87.00
Tween ® 20
For Laboratory Use.
2.5 ml W3831 53.00
Description: This BSA (bovine serum albumin) has been tested and qualified
for optimum performance in immunoblotting applications with alkaline phosphatase
antibody conjugates. It is shown to be alkaline phosphatase-free.
Tween ® 20 is a nonionic detergent used as a buffer component for immunoscreening
in the ProtoBlot ® Systems. In addition to blocking agents
such as BSA, which saturate excess sites of antibody binding on membranes,
this detergent acts in solution to dissociate nonspecific interactions with an
antibody probe.
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