2012 Promega catalogue

Cell Signaling
Inducible T7-Driven Flexi ® Vectors for E. coli
Expression
Product Size Cat.# Price ($)
pF1A T7 Flexi ® Vector 20 µg C8441 435.00
pF1K T7 Flexi ® Vector 20 µg C8451 435.00
pFN18A HaloTag ® T7 Flexi ® Vector 20 µg G2751 397.00
pFN18K HaloTag ® T7 Flexi ® Vector 20 µg G2681 397.00
pFN19A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1891 397.00
pFN19K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1841 397.00
pFC20A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1681 397.00
pFC20K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1691 397.00
pFN2A (GST) Flexi ® Vector 20 µg C8461 489.00
pFN2K (GST) Flexi ® Vector 20 µg C8471 489.00
pFN6A (HQ) Flexi ® Vector 20 µg C8511 489.00
pFN6K (HQ) Flexi ® Vector 20 µg C8521 489.00
pFC7A (HQ) Flexi ® Vector 20 µg C8531 489.00
pFC7K (HQ) Flexi ® Vector 20 µg C8541 489.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: These Flexi ® vectors are designed specifically for expression of
proteins in E. coli from the T7 RNA polymerase promoter with or without the
addition of purification tags. Expression levels can vary from protein to protein,
but in general the N-terminal HaloTag ® fusion protein (e.g., pFN18A/K) can
increase expression by increasing solubility of the expressed protein. Proteins
may be purified using the HaloTag ® fusion with the HaloTag ® Protein Purification
System (Cat.# G6280). Expression may be confirmed through cell-free expression
with the S30 T7 High-Yield Protein Expression System (Cat.# L1110).
Note: Flexi ® Vectors contain the lethal barnase gene to reduce background colonies
without inserts during the subcloning procedure. The Flexi ® Vector
Cloning System replaces the barnase gene with your insert. These vectors, as purchased,
cannot be cultured in normal laboratory strains of E. coli without an insert.
Features:
• Versatility: You can choose between a variety of initial applications (e.g.,
bacterial protein, mammalian, or cell-free protein expression) and then
transfer to others as required.
• Time Savings: Efficient transfer allows for direct use of recombinant
clones, minimizing time wasted screening background colonies.
• Enhanced Productivity: Adaptable to high-throughput formats for large
screening projects.
• Easy Access: No licensing fees or complicated transfer restrictions.
Storage Conditions: Store vectors at –20°C.
For complete and up-to-date product information visit: www.promega.com/catalog
FluoroTect Green Lys in vitro Translation
Labeling System
Product
FluoroTect GreenLys in vitro Translation
Size Cat.# Price ($)
Labeling System 40 reactions L5001 646.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The FluoroTect Green Lys in vitro Translation Labeling System
allows for the fluorescent labeling and detection of proteins synthesized in vitro.
The system is based on a lysine-charged tRNA that is labeled at the ε position
of the lysine with the fluorophore BODIPY ® -FL. Fluorescent lysine residues will
be incorporated into synthesized proteins during in vitro translation reactions,
eliminating the need for radioactivity.
Detection of the labeled proteins is accomplished in 2–5 minutes directly
“in-gel” by use of a laser-based fluorescent gel scanner. This eliminates any
requirements for protein gel manipulation such as fixing/drying or any safety,
regulatory and waste disposal issues associated with the use of radioactively
labeled amino acids use. The convenience of “in-gel” detection also avoids
the time-consuming electroblotting and detection steps of conventional nonisotopic
systems.
Features:
• Fast: Data can be obtained in minutes, eliminating overnight exposures associated
with radioactive-based systems or time-consuming steps utilized
by traditional non-isotopic methodologies.
• Convenient: Results based on “in-gel” detection. No requirement to
transfer, fix, or dry gels.
• Non-Radioactive: No safety, regulatory or waste disposal issues associated
with radioactivity.
• Flexible: The modified charged tRNA can be used with a variety of
Promega translation systems including: Rabbit Reticulocyte Lysate, TnT ®
Coupled Transcription/Translation System, Wheat Germ Extract and E. coli
S30 Extract.
Storage Conditions: Store at –70°C.
Protocol Part#
FluoroTect GreenLys in vitro Translation Labeling System Technical
Bulletin
TB285
237
13
Protein Expression and Analysis
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