2012 Promega catalogue

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Life Science Catalog <strong>2012</strong> Worldwide Contact List Section Contents Table of Contents Cell Signaling 236 HaloTag ® Fusion (N-Terminal) Mammalian Expression Vectors Product Size Cat.# Price ($) pHTN HaloTag ® CMV-neo Vector 20 µg G7721 355.00 pFN28A HaloTag ® CMV-neo Flexi ® Vector 20 µg G8441 397.00 pFN28K HaloTag ® CMV-neo Flexi ® Vector 20 µg G8451 397.00 pFN21A HaloTag ® CMV Flexi ® Vector 20 µg G2821 397.00 pFN21K HaloTag ® CMV Flexi ® Vector 20 µg G2831 397.00 pFN22A HaloTag ® CMVd1 Flexi ® Vector 20 µg G2841 397.00 pFN22K HaloTag ® CMVd1 Flexi ® Vector 20 µg G2851 397.00 pFN23A HaloTag ® CMVd2 Flexi ® Vector 20 µg G2861 397.00 pFN23K HaloTag ® CMVd2 Flexi ® Vector 20 µg G2871 397.00 pFN24A HaloTag ® CMVd3 Flexi ® Vector 20 µg G2881 397.00 pFN24K HaloTag ® CMVd3 Flexi ® Vector 20 µg G2981 397.00 Available Separately HaloTag ® Cloning Starter System 1 each G6050 568.00 HaloTag ® Flexi ® Vectors—CMV Deletion Series Sample Pack For Research Use Only. Not for Use in Diagnostic Procedures. 9 × 2 µg G3780 333.00 Description: The HaloTag ® vectors are designed for expression of C-terminal or N-terminal-tagged HaloTag ® fusion proteins in mammalian cells. Once expressed, the HaloTag ® fusion protein may be used for imaging of protein localization or trafficking in conjunction with the fluorescent HaloTag ® Ligands. In addition, the HaloTag ® fusion protein can be purified or pulled down as a complex with its protein partners. We offer two types of HaloTag ® fusion vectors to accommodate your cloning preferences: • pHT Vector Series: Simple Multiple Cloning Site (MCS) plasmids for traditional cloning. • pF Vector Series: Flexi ® Vector Cloning System---a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and highfidelity way to transfer protein-coding regions between a variety of Flexi ® Vectors without the need to resequence. Note: Flexi ® Vectors (pF series) contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. Using the Flexi ® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. Relative Mammalian Protein Expression Levels for HaloTag ® Flexi ® Vectors. Vector Name Cat.# Expression Level* pFC14A HaloTag ® CMV Flexi ® Vector pFC14K HaloTag ® CMV Flexi ® Vector pFC15A HaloTag ® CMVd1 Flexi ® Vector pFC15K HaloTag ® CMVd1 Flexi ® Vector pFC16A HaloTag ® CMVd2 Flexi ® Vector pFC16K HaloTag ® CMVd2 Flexi ® Vector pFC17A HaloTag ® CMVd3 Flexi ® Vector pFC17K HaloTag ® CMVd3 Flexi ® Vector pFN21A HaloTag ® CMV Flexi ® Vector pFN21K HaloTag ® CMV Flexi ® Vector pFN22A HaloTag ® CMVd1 Flexi ® Vector pFN22K HaloTag ® CMVd1 Flexi ® Vector pFN23A HaloTag ® CMVd2 Flexi ® Vector pFN23K HaloTag ® CMVd2 Flexi ® Vector pFN24A HaloTag ® CMVd3 Flexi ® Vector pFN24K HaloTag ® CMVd3 Flexi ® Vector pFC27A HaloTag ® CMV-neo Flexi ® Vector pFC27K HaloTag ® CMV-neo Flexi ® Vector pFN28A HaloTag ® CMV-neo Flexi ® Vector pFN27K HaloTag ® CMV-neo Flexi ® Vector G9651 High G9661 High G1611 Medium G1601 Medium G1591 Low G1571 Low G1551 Ultra-Low G1321 Ultra-Low G2821 High G2831 High G2841 Medium G2851 Medium G2861 Low G2871 Low G2881 Ultra-Low G2981 Ultra-Low G8421 High 1 G8431 High 2 G8441 High 1 G8451 High 2 For complete and up-to-date product information visit: www.promega.com/catalog 9161LA 1 This vector confers ampicillin resistance for Flexi ® cloning, and the neomycin (G418) selection cassette affords antibiotic selection of stable cell lines expressing the HaloTag ® fusion protein. 2 This vector confers kanamycin resistance for Flexi ® cloning, and the neomycin (G418) selection cassette affords antibiotic selection of stable cell lines expressing the HaloTag ® fusion protein. *Expression level depends on the cell type and the protein fused to HaloTag ® protein.
Cell Signaling Inducible T7-Driven Flexi ® Vectors for E. coli Expression Product Size Cat.# Price ($) pF1A T7 Flexi ® Vector 20 µg C8441 435.00 pF1K T7 Flexi ® Vector 20 µg C8451 435.00 pFN18A HaloTag ® T7 Flexi ® Vector 20 µg G2751 397.00 pFN18K HaloTag ® T7 Flexi ® Vector 20 µg G2681 397.00 pFN19A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1891 397.00 pFN19K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1841 397.00 pFC20A HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1681 397.00 pFC20K HaloTag ® T7 SP6 Flexi ® Vector 20 µg G1691 397.00 pFN2A (GST) Flexi ® Vector 20 µg C8461 489.00 pFN2K (GST) Flexi ® Vector 20 µg C8471 489.00 pFN6A (HQ) Flexi ® Vector 20 µg C8511 489.00 pFN6K (HQ) Flexi ® Vector 20 µg C8521 489.00 pFC7A (HQ) Flexi ® Vector 20 µg C8531 489.00 pFC7K (HQ) Flexi ® Vector 20 µg C8541 489.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: These Flexi ® vectors are designed specifically for expression of proteins in E. coli from the T7 RNA polymerase promoter with or without the addition of purification tags. Expression levels can vary from protein to protein, but in general the N-terminal HaloTag ® fusion protein (e.g., pFN18A/K) can increase expression by increasing solubility of the expressed protein. Proteins may be purified using the HaloTag ® fusion with the HaloTag ® Protein Purification System (Cat.# G6280). Expression may be confirmed through cell-free expression with the S30 T7 High-Yield Protein Expression System (Cat.# L1110). Note: Flexi ® Vectors contain the lethal barnase gene to reduce background colonies without inserts during the subcloning procedure. The Flexi ® Vector Cloning System replaces the barnase gene with your insert. These vectors, as purchased, cannot be cultured in normal laboratory strains of E. coli without an insert. Features: • Versatility: You can choose between a variety of initial applications (e.g., bacterial protein, mammalian, or cell-free protein expression) and then transfer to others as required. • Time Savings: Efficient transfer allows for direct use of recombinant clones, minimizing time wasted screening background colonies. • Enhanced Productivity: Adaptable to high-throughput formats for large screening projects. • Easy Access: No licensing fees or complicated transfer restrictions. Storage Conditions: Store vectors at –20°C. For complete and up-to-date product information visit: www.promega.com/catalog FluoroTect Green Lys in vitro Translation Labeling System Product FluoroTect GreenLys in vitro Translation Size Cat.# Price ($) Labeling System 40 reactions L5001 646.00 For Research Use Only. Not for Use in Diagnostic Procedures. Description: The FluoroTect Green Lys in vitro Translation Labeling System allows for the fluorescent labeling and detection of proteins synthesized in vitro. The system is based on a lysine-charged tRNA that is labeled at the ε position of the lysine with the fluorophore BODIPY ® -FL. Fluorescent lysine residues will be incorporated into synthesized proteins during in vitro translation reactions, eliminating the need for radioactivity. Detection of the labeled proteins is accomplished in 2–5 minutes directly “in-gel” by use of a laser-based fluorescent gel scanner. This eliminates any requirements for protein gel manipulation such as fixing/drying or any safety, regulatory and waste disposal issues associated with the use of radioactively labeled amino acids use. The convenience of “in-gel” detection also avoids the time-consuming electroblotting and detection steps of conventional nonisotopic systems. Features: • Fast: Data can be obtained in minutes, eliminating overnight exposures associated with radioactive-based systems or time-consuming steps utilized by traditional non-isotopic methodologies. • Convenient: Results based on “in-gel” detection. No requirement to transfer, fix, or dry gels. • Non-Radioactive: No safety, regulatory or waste disposal issues associated with radioactivity. • Flexible: The modified charged tRNA can be used with a variety of <strong>Promega</strong> translation systems including: Rabbit Reticulocyte Lysate, TnT ® Coupled Transcription/Translation System, Wheat Germ Extract and E. coli S30 Extract. Storage Conditions: Store at –70°C. Protocol Part# FluoroTect GreenLys in vitro Translation Labeling System Technical Bulletin TB285 237 13 Protein Expression and Analysis Section Contents Table of Contents
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Life Science CATALOG 2012
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Cell Signaling Table of Contents Pr
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Cell Signaling For complete and up-
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Cell Signaling Agarose, LMP, Prepar
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Cell Signaling Blue/Orange Loading
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Cell Signaling Glycine, Molecular B
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Cell Signaling Sephacryl ® S-400 P
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Cell Signaling Tris Base, Molecular
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Cell Signaling Unmethylated Lambda
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Cell Signaling Promega Barrier Tips
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Cell Signaling Product Size Cat.# P
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Cell Signaling 2 Cell Health Assays
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Cell Signaling A. B. Luminescence/p
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Cell Signaling Luminogenic Enzyme S
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Cell Signaling Apoptosis
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Cell Signaling Caspase-Glo ® 2 Ass
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Cell Signaling Caspase-Glo ® 9 Ass
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Cell Signaling CaspACE Assay System
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Cell Signaling Anti-ACTIVE ® Caspa
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Cell Signaling BacTiter-Glo Microbi
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Cell Signaling CellTiter 96 ® Cell
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Cell Signaling MultiTox-Fluor Multi
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Cell Signaling CytoTox-Glo Cytotoxi
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Cell Signaling CytoTox 96 ® Non-Ra
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Cell Signaling GSH-Glo Glutathione
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Cell Signaling 3 Cell Signaling For
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Cell Signaling cAMP-Glo Max Assay P
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Cell Signaling Growth Factors Epide
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Cell Signaling SIRT-Glo Assays and
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Cell Signaling ADP-Glo Max Assay Pr
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase Enzyme System
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Cell Signaling Kinase-Glo ® Kinase
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Cell Signaling SignaTECT ® Protein
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Cell Signaling Casein Kinase I Prod
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Cell Signaling Product Size Cat.# P
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Cell Signaling Protein Kinase Subst
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Cell Signaling DNA Step Ladders Pro
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Cell Signaling Conventional DNA Mar
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Cell Signaling ProMega-Markers ® L
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Cell Signaling Restriction Enzymes
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Cell Signaling BamHI Product Size C
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Cell Signaling EcoRI Product Size C
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Cell Signaling NciI Product Size Co
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Cell Signaling SpeI Product Size Co
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Cell Signaling TSAP Thermosensitive
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Cell Signaling T3 RNA Polymerase Pr
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Cell Signaling Kinases T4 Polynucle
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Cell Signaling S1 Nuclease Product
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Cell Signaling Percent Inhibition o
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Cell Signaling Untagged Flexi ® Ma
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Cell Signaling pGEM ® -5Zf(+) Vect
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Cell Signaling pSP64 Poly(A) Vector
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Cell Signaling x-tracta Gel Extract
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Cell Signaling Wizard ® MagneSil
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Cell Signaling ReliaPrep FFPE gDNA
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Cell Signaling MagneSil ® ONE, Fix
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Cell Signaling MagneSil ® KF, Geno
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Cell Signaling Plasmid Purification
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Cell Signaling PureYield Plasmid Ma
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Cell Signaling Wizard ® Plus Maxip
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Cell Signaling Wizard ® MagneSil
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Cell Signaling PureYield RNA Midipr
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Cell Signaling Maxwell ® 16 System
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Cell Signaling RNasin ® Plus RNase
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Cell Signaling DNA Quantitation Qua
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Cell Signaling Magnetic Stands and
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Cell Signaling 6 Drug Discovery For
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Cell Signaling GPCR Assays cAMP-Glo
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Cell Signaling PDE-Glo Phosphodiest
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Cell Signaling Protease Assays Prot
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Cell Signaling Cell-Based Proteasom
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Cell Signaling Tryptase, Human, Rec
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Cell Signaling 7 Genetic Identity F
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Cell Signaling Maxwell ® 16 Forens
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Cell Signaling Plexor ® HY System
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Cell Signaling PowerPlex ® 18D Sys
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Cell Signaling PowerPlex ® CS7 Sys
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Cell Signaling PowerPlex ® 16 and
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Cell Signaling GenePrint ® Fluores
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Cell Signaling HaloTag ® Ligand Bu
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Cell Signaling Block & Sample 5X Bu
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Cell Signaling Anti-ACTIVE ® JNK p
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Cell Signaling Anti-ERK 1/2 pAb, Ra
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Cell Signaling Anti-Human GDNF pAb
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Cell Signaling Anti-PARP p85 Fragme
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Cell Signaling Anti-VAChT pAb Produ
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Cell Signaling VivoGlo Caspase 3/7
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Cell Signaling Microbial Detection
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10 Instruments For complete and up-
Page 189 and 190: GloMax ® 20/20 Luminometer Product
Page 191 and 192: Fluorescence Module: Application-op
Page 193 and 194: GloMax ® -Multi Jr Single-Tube Mul
Page 195 and 196: Maxwell ® 16 Instruments Maxwell
Page 197 and 198: Maxwell ® 16 Flexi Method Firmware
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Page 201 and 202: Cell Signaling Maxwell ® 16 System
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Page 205 and 206: Cell Signaling dNTP Mix Product Siz
Page 207 and 208: Cell Signaling 12 PCR For complete
Page 209 and 210: Cell Signaling TaqBead Hot Start Po
Page 211 and 212: Cell Signaling GoTaq ® PCR Core Sy
Page 213 and 214: Cell Signaling Deoxynucleotide Trip
Page 215 and 216: Cell Signaling Plexor ® qPCR and q
Page 217 and 218: Cell Signaling StemElite Differenti
Page 219 and 220: Cell Signaling Reverse Transcriptio
Page 221 and 222: Cell Signaling AMV Reverse Transcri
Page 223 and 224: Cell Signaling PCR Cloning pGEM ®
Page 227 and 228: Cell Signaling TnT ® Quick Coupled
Page 229 and 230: Cell Signaling TnT ® T7 Quick for
Page 231 and 232: Cell Signaling Canine Pancreatic Mi
Page 233 and 234: Cell Signaling pGEM ® β-Gal Contr
Page 235 and 236: Cell Signaling Immobilized Trypsin
Page 237 and 238: Cell Signaling Asp-N, Sequencing Gr
Page 239: Cell Signaling HaloTag ® Ligand Bu
Page 243 and 244: Cell Signaling ProtoBlot ® II AP S
Page 247 and 248: Cell Signaling HaloTag ® Protein P
Page 249 and 250: Cell Signaling HaloTag ® Mammalian
Page 251 and 252: Cell Signaling HaloLink Protein Arr
Page 253 and 254: Cell Signaling HaloCHIP System Prod
Page 255 and 256: Cell Signaling MagneHis Protein Pur
Page 257 and 258: Cell Signaling Streptavidin Product
Page 259 and 260: Cell Signaling Protein Purification
Page 261 and 262: Cell Signaling 15 Reporter Assays a
Page 263 and 264: Cell Signaling Dual-Glo ® Lucifera
Page 265 and 266: Cell Signaling Firefly Luciferase R
Page 267 and 268: Cell Signaling Bright-Glo Luciferas
Page 269 and 270: Cell Signaling EnduRen Live Cell Su
Page 271 and 272: Cell Signaling Beta-Glo ® Assay Sy
Page 273 and 274: Cell Signaling Promoter-Driven Cont
Page 275 and 276: Cell Signaling Nuclear Receptor Pat
Page 277 and 278: Cell Signaling pRL Renilla Lucifera
Page 279 and 280: Cell Signaling GloResponse Lucifera
Page 281 and 282: Cell Signaling pSP-luc+NF Fusion Ve
Page 283 and 284: Cell Signaling VivoGlo Caspase 3/7
Page 285 and 286: Cell Signaling Transfection Reagent
Page 287 and 288: Cell Signaling 16 RNA Analysis For
Page 289 and 290: Cell Signaling Riboprobe ® System
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Cell Signaling HeLaScribe ® Nuclea
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Cell Signaling RNA Interference Gen
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Cell Signaling 17 Stem Cell Researc
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Cell Signaling Cell ID System Produ
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Cell Signaling StemElite Human Panc
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Cell Signaling 18 Vectors For compl
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Cell Signaling HaloTag ® Fusion (N
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Cell Signaling pCI Mammalian Expres
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Cell Signaling Reporter Vectors pGL
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Cell Signaling pGEM ® -luc DNA Pro
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Cell Signaling pGEM ® and pSP Subc
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Cell Signaling pUC/M13 Sequencing P
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Index: A-Z 19 Index For complete an
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BssHII.............................
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In vitro Transcription Systems Rela
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QuantiFluor dsDNA System ..........
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Cat# Product Size Page Price($) A10
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Index by Catalog Number Cat# Produc
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Cat# Product Size Page Price($) C84
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Cat# Product Size Page Price($) E13
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Cat# Product Size Page Price($) E61
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Cat# Product Size Page Price($) G13
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Cat# Product Size Page Price($) G77
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Cat# Product Size Page Price($) L50
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Cat# Product Size Page Price($) P16
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Cat# Product Size Page Price($) R99
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Cat# Product Size Page Price($) V42
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Cat# Product Size Page Price($) W39
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personal DETECTION GloMax Integrat
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