2012 Promega catalogue

Life
Science
Catalog
2012
Worldwide Contact List
Section
Contents
Table of
Contents
Cell Signaling
ADME Assays
20
CYP450 Assay Systems
Product Size Cat.# Price ($)
P450-Glo CYP1A2 Induction/Inhibition Assay 10 ml V8421 181.00
50 ml V8422 523.00
P450-Glo CYP3A4 Assay with Luciferin-IPA 10 ml V9001 181.00
50 ml V9002 523.00
P450-Glo CYP3A4 Assay (Luciferin-PPXE)
10 ml V8911 181.00
DMSO-Tolerant Assay
50 ml V8912 523.00
P450-Glo CYP3A4 Assay (Luciferin-PFBE)
10 ml V8901 181.00
Cell-Based/Biochemical Assay
50 ml V8902 523.00
P450-Glo CYP1A1 Assay 10 ml V8751 181.00
50 ml V8752 523.00
P450-Glo CYP1B1 Assay 10 ml V8761 181.00
50 ml V8762 523.00
P450-Glo CYP1A2 Assay 10 ml V8771 181.00
50 ml V8772 523.00
P450-Glo CYP2C8 Assay 10 ml V8781 181.00
50 ml V8782 523.00
P450-Glo CYP2C9 Assay 10 ml V8791 181.00
50 ml V8792 523.00
P450-Glo CYP3A4 Assay 10 ml V8801 181.00
50 ml V8802 523.00
P450-Glo CYP3A7 Assay 10 ml V8811 181.00
50 ml V8812 523.00
P450-Glo CYP2C19 Assay 10 ml V8881 181.00
50 ml V8882 523.00
P450-Glo CYP2D6 Assay 10 ml V8891 181.00
Available Separately
50 ml V8892 523.00
NADPH Regeneration System 1,000 assays V9510 180.00
For Research Use Only. Not for Use in Diagnostic Procedures.
Description: The P450-Glo CYP450 Assays provide a homogeneous,
luminescent method for measuring cytochrome P450 activity. The assays are
designed to measure the activities of P450s from recombinant and native
sources and for testing the effects of analytes such as drugs and new chemical
entities on P450 activities. These luminescent assays exhibit exquisite
sensitivity, low background signals and broad dynamic range.
P450-Glo Assays employ luminogenic P450 substrates that are derivatives
of beetle luciferin. The derivatives are not substrates for luciferase but are
converted by P450s to luciferin, which in turn reacts with luciferase to produce
light that is directly proportional to the activity of the P450.
The P450-Glo Assays generate a “glow-type” luminescent signal, produced
using a recombinant stabilized luciferase (Ultra-Glo Luciferase) coupled with
a proprietary buffer system. The half-life of the luminescent output is greater
than two hours, eliminating the need for luminometers with injectors and
allowing batch plate processing. The formulation also minimizes the incidence
of false positives due to inhibition of luciferase by analytes when screening for
cytochrome P450 inhibitors.
The new P450-Glo CYP3A4 Assay with Luciferin-IPA contains a new
substrate for cytochrome 3A4 that is very well suited for all applications
involving human CYP3A4 and is the best substrate available for cell-based
applications. Luciferin-IPA is readily taken up by cells and rapidly converted into
luciferin inside the cell, which reduces the incubation time required. The low
background and high signal-to-noise ratio produced using Luciferin-IPA means
less starting material is required.
Dimethyl sulfoxide (DMSO) can inhibit the activity of the 3A4 isoform of cytochrome
P450, even at low concentrations (